Optically Clear Heat Seal

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Optically Clear Heat Seal

Overview

Optically Clear Heat Seal Performance

 

The optically clear heat seal prevents sample evaporation that can lead to large Cq standard deviations. Five-color multiplex linearity test demonstrates highly reliable qPCR results. A–E, fluorescence data from a series of tenfold dilutions of plasmid DNA (108–102 copies) amplified using reporter dyes to monitor five targets. The standard curves of five multiplexed genes have an R2 >0.990. F, standard curves generated from data in A–E. Reaction efficiencies are between 90 and 105%. Cq, quantification cycle; RFU, relative fluorescence units.

Suggested Heat Sealing Parameters

 

        Optically Clear Heat Seal Permanent Clear Heat Seal Pierceable Foil Heat Seal Peelable Foil Heat Seal
Product Catalog Number Number of Wells Well Color Temp, °C Time, sec Temp, °C Time, sec Temp, °C Time, sec Temp, °C Time, sec
Hard-Shell® 384-well standard PCR plates HSP-3xxx 384 Clear

White
175

167
3

3
175

167
3

3
170

170
3

3
185

185
4

3
Hard-Shell 384-well 480 PCR plates HSR-4xxx 384 Clear

White
175

167
3

3
175

167
3

3
170

170
3

3
185

185
4

3
Hard-Shell low-profile 96-well skirted PCR plates HSP-9xxx 96 Clear

White
185

180
3

3
180

180
3

3
175

175
3

3
180

185
3

5
Hard-Shell high-profile 96-well semi-skirted PCR plates HSS-9xxx 96 Clear

White
170

185
3

3
165

180
3

3
170

175
3

3
185

185
3

3
Multiplate™ low-profile 96-well unskirted PCR plates MLL-9xxx 96 Clear

White
180

180
3

3
180

160
3

3
175

175
3

3
185

185
5

3
Multiplate high-profile 96-well unskirted PCR plates MLP-9xxx 96 Clear

White
175

170
3

3
150

165
3

3
175

175
3

3
185

185
3

3
iQ™ 96-well PCR plates 223-9441 96 Clear 180 3 180 3 185 3 185 5

 

Heat Sealing Films and Foils
  Optically Clear
Heat Seal
Permanent Clear
Heat Seal
Pierceable Foil
Heat Seal
Peelable Foil
Heat Seal
Catalog number 181-4030 181-4035 181-4040 181-4045
Applications – Real-time PCR
– PCR
– Water bath cycling
– PCR
– QX100™ Droplet Digital™
  PCR system
– PCR
– Sample storage
– PCR
Maximum temperature 110°C 110°C 110°C 110°C
Minimum temperature –80°C –80°C –20°C –200°C
Peelable seal +   + +
Pierceable seal     +  
DNase and RNase free + + + +
Sealing compatibility PP, COC, PE,* PS PP PP, PS* PP, COC*
COC, cyclic olefin copolymer; PE, polyethylene; PP, polypropylene; PS, polystyrene.
* Limited and variable compatibility based on plate composition.

PX1™ PCR Plate Sealer FAQs

Which heat seal is best for my PCR application?
Use the Heat Sealing Films and Foils table to determine the seal that best suits your experimental needs.

 

How do I determine the best sealing time and temperature?
Optimal sealing parameters vary according to the heat films and microplates used. We recommend sealing an empty plate using the guidelines found in Suggested Heat Sealing Parameters. After sealing, examine the entire plate for effective sealing and optimize sealing conditions if necessary.

 

Some of my wells are not fully sealed. What should I do?
Sealing temperature and/or sealing time may be increased to help improve sealing performance. Use an empty plate to optimize seal conditions. See the instruction manual for additional details about seal optimization.

 

When should I use the sealing frame?
The sealing frame helps secure the heat seal in place and prevents the film from moving during sealing. The sealing frame must be used for the permanent clear heat seal and the peelable foil heat seal.

 

What happens if I accidently place a seal upside down when sealing?
The PX1 heated platen was designed with an easy to clean, nonstick surface. See the PX1™ PCR Plate Sealer Instruction Manual for directions on cleaning the heated platen. Ensure the PX1 sealer cools for at least 1 hour before cleaning.

 

Is it safe to leave the heat sealer on when unattended?
Yes. The PX1 PCR plate sealer was designed for reliability and safety. The instrument has an adjustable auto-off feature that automatically shuts the heater off when not in use for a designated amount of time. Simply touch the screen and the instrument will automatically resume heating.

 

Can I reseal the same plate twice?
A plate sealed with a peelable film may be resealed after peeling an existing film. However, resealing a plate for subsequent use in real-time PCR is not recommended. A plate sealed with a pierceable foil heat seal may also be resealed by sealing another pierceable foil heat seal directly on top. Note: only a single seal can be used in the QX100™ Droplet Digital™ PCR system workflow. Sealing parameters may need to be adjusted.

 

The current platen temperature is higher than the required sealing temperature. How can I reduce the time needed to reach sealing temperature?
The heated platen cools passively. Opening the PX1 PCR plate sealer drawer can help accelerate the cooling process.

 

Can I use traditional adhesive or pressure-sensitive seals with the PX1 sealer?
No. Our heat sealing films feature a special hot melt adhesive that effectively seals microplates when used with the PX1 sealer. The temperature and pressure delivered by the PX1 will not successfully seal a microplate when traditional adhesive and pressure-sensitive seals are used.

Amplification Reagents and Plastics Brochure
The Amplification Reagents and Plastics Brochure includes information about

 

  • Products for gene expression analysis


    RNA Isolation


    Reverse Transcription Reagents


    Real-Time qPCR Reagents


    PCR Plastic Consumables

  • Product performance data

  • Quick and easy product selection tables

  • Ordering information

 

Download your free copy of the Amplification Reagents and Plastics Brochure.

The optically clear heat seal provides a secure sealing option for standard and real-time PCR applications. This clear polymer film enables easy inspection of sample wells. The seal is peelable pre- and post-cycling, allowing easy sample retrieval when desired.

Key Features and Benefits

  • Ideal for optical assays — high light transmission
  • Excellent for qPCR — low levels of autofluorescence
  • Fully validated — minimal sample evaporation when used with the PX1™ PCR plate sealer
  • Versatile — compatible with a variety of PCR plastic materials, including polypropylene, polyethylene, polystyrene, and some cyclic olefin copolymer materials

Additional Details

  • Seal integrity from –80 to 110°C
  • Seals are packaged interleafed with dust-free paper for easy handling
  • Suggested Heat Sealing Parameters guide is available to help minimize optimization steps
  • Certified RNase and DNase free

Bio-Rad's Heat Sealing Films and Foils

Bio-Rad offers several heat sealing films and foils, each of which is optimal for specific PCR applications.

Heat Seal Application
Optically clear heat seal Best suited for thermal cycling and real-time PCR applications when a clear, peelable seal is desired
Permanent clear heat seal Provides the strongest heat sealing option and is ideal for thermal cycling experiments where seals do not need to be removed
Pierceable foil heat seal Ideal for thermal cycling and workflows requiring a pierceable seal, such as Droplet Digital™ PCR applications
Peelable foil heat seal Best for thermal cycling and sample storage
Find out more about the specific features and specifications for each of the heat sealing films and foils.

Optically Clear Heat Seal

181-4030
Pkg of 100, optically clear seals for PCR and real-time PCR applications, for use with PX1™ PCR plate sealer

List Price:   $107.00
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Number Description Options
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
10025819 Optically Clear Heat Seal Product Insert, Rev A Click to download
10023997 Instruction Manual, PX1 PCR Plate Sealer, Rev A Click to download
6257 PX1 PCR Plate Sealer and Heat Sealing Consumables Brochure, Rev A Click to download