One-Step RT-ddPCR Advanced Kit for Probes

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One-Step RT-ddPCR Advanced Kit for Probes


This one-step reverse transcription digital PCR supermix delivers improved efficiency, specificity, and sensitivity for precise RNA target quantification with Droplet Digital™ PCR.

Key Benefits

  • Absolute quantification by Droplet Digital PCR in a convenient single-reaction format
  • Contains all components required for hydrolysis probe–based RT-ddPCR except primers, probe(s), and template
  • Limits nonspecific PCR amplification
  • Optimized enzyme blend enables partitioning of RNA samples into droplets while keeping the enzymes inactive until the reverse transcription reaction is performed at 50°C
  • Contains RNase inhibitor to protect the RNA throughout the entire workflow

Applications and Uses

  • Absolute quantification
  • Gene expression analysis
  • Viral load detection
  • Pathogen detection

Packaging Options

Number of 20 µl Reactions Catalog Number
200 186-4021
500 186-4022

Additional Reagents

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of one or more of the following instruments and accessories:

Related Products

Bio-Rad offers additional digital PCR supermixes including:

More Information

This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX200™ Droplet Digital™ PCR System and QX200™ AutoDG™ Droplet Digital™ System.

Storage at –20°C Up to 12 months (refer to expiration date)
Template compatibility RNA
x

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One-Step RT-ddPCR Advanced Kit for Probes

1864021
200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

List Price:
 
$1,010.00
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One-Step RT-ddPCR Advanced Kit for Probes

1864022
500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

List Price:
 
$2,525.00
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Number Description Options
10049226 One-Step RT-ddPCR Advanced Kit for Probes Product Insert, Rev A Click to download
10040718 Droplet Generation Oil for Probes Product Insert, Rev B Click to download
6311 QX200™ Droplet Digital™ PCR System Brochure, Rev C Click to download [ Add to Cart (Free) ]
6450 Droplet Digital™ PCR Publications List, Rev L Click to download
10043138 Instruction Manual, Automated Droplet Generator for Droplet Digital™ PCR Systems, Rev B Click to download
10031906 Instruction Manual, QX200™ Droplet Reader and QuantaSoft™ Software, Rev D Click to download