Nuvia™ IMAC Metal Affinity Resin

Print


Nuvia IMAC Resin is a metal chelate affinity chromatography resin that contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The inert hydrophilic surface of the base bead and the chemical structure of NTA ensure highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+, or with other transition metals such as Zn2+ or Cu2+. The optimized particle size and high porosity allows operation at increasingly higher flow rates without compromising binding capacity. Nuvia IMAC can be used at any scale from laboratory to manufacturing, providing robust, reproducible, and efficient purifications each time.

Nuvia IMAC Resin is stable in a broad range of chemicals. It is compatible with all reagents traditionally used for histidine-tagged protein purification, including reducing agents, detergents, denaturing agents, and additives.

Features and Benefits

  • Easy and efficient scale up from lab to manufacturing scale purifications
  • Chelating ligand nitrilotriacetic acid (NTA) may be charged with transition metals such as Cu2+, Ni2+, Co2+, or Zn2+
  • Optimized particle size for high flow rate and low backpressure
  • Superior dynamic binding capacity at high flow rates
  • Withstands repeated clean-in-place/recharging cycles
  • Robust recovery and reproducibility
  • Full regulatory support

Applications and Uses of Nuvia IMAC Resin

For purifying biomolecules in a variety of workflows:

  • Excellent single-step purity via affinity chromatography
  • Quick, efficient separation of biomolecules
  • Contaminant removal
  • Robust base matrix

Nuvia IMAC Resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.

Product Options

Package Size Catalog Number
Nuvia IMAC Ni-Charged Support, bulk resin  
25 ml 7800800
100 ml 7800801
500 ml 7800802
5 L 12003233
10 L 12002782
Nuvia IMAC Ni-Charged Support, prepacked  
Prepacked column, 1 ml 12004038
Prepacked column, 5 ml 12004037
Prepacked filter plates, 20 µl 12004035
Prepacked RoboColumns, 200 µl 12004051
Prepacked RoboColumns, 600 µl 12004052
Nuvia IMAC Uncharged Support, bulk resin  
5 L 12004040
10 L 12004039
 

More Information

Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage. Prepacked Bio-Scale™ Mini Nuvia IMAC Cartridges with Ni-charged NTA are available.

Particle size 38–53 µm
Ligand density ≥ 18 µmol/ml
Dynamic binding capacity* > 40 mg/ml at 300 cm/hr
*10% breakthrough capacity determined with 1.2 mg/ml of a 40 kD histidine-tagged protein in 50 mM sodium phosphate, 5 mM imidazole, 300 mM NaCl (pH 7.5).
Compression factor 1.20–1.25
Recommended linear flow rate range 50–300 cm/hr
Pressure vs. flow performance Under 2 bar at flow rate of 300 cm/hr in DI water (20 x 20 cm packed bed, 1.2 compression factor)
pH stability 2–14
Shipping solution 2% benzyl alcohol or 20% ethanol
Regeneration 50 mM EDTA, pH 8.0 (Stripping)
1N NaOH (CIP/SIP)
0.1 M Ni2SO4 (Recharging)
Cleaning-in-place (CIP) solutions 1N NaOH
Sanitization 1N NaOH
Storage condition 20% Ethanol
Chemical compatibility Reducing agents (β-ME, TCEP, DTT)
Denaturing agents (GnHCl)
Detergents (Triton X-100, NP-40, CHAPS, CHAPSO)
Additives (glycerol)
**No recharge required. Refer to the instruction manual for a complete list.
Chemical stability 48 hr with 1M NaOH, 1 week with 0.01M HCl
***Recharge required. Refer to the instruction manual for a complete list.
x

Account price failed to display. Please try reloading the page. Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at   or send an
email to 

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Nuvia IMAC Resin
Nuvia™ IMAC Resin, 25 ml

7800800
Pkg of 1, 25 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

List Price:
 
$310.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Nuvia IMAC Resin
Nuvia™ IMAC Resin, 100 ml

7800801
Pkg of 1, 100 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

List Price:
 
$1,040.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Nuvia IMAC Resin
Nuvia™ IMAC Resin, 500 ml

7800802
Pkg of 1, 500 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

List Price:
 
$4,650.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Nuvia™ IMAC Resin, 5 L

12003233
Pkg of 1, 5 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

List Price:
 
Inquire
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Quote
Add to Hot List  
Nuvia™ IMAC Resin, 10 L

12002782
Pkg of 1, 10 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

List Price:
 
Inquire
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Quote
Add to Hot List  
Nuvia™ IMAC Uncharged Resin, 5 L
Nuvia™ IMAC Uncharged Resin, 5 L

12004040
Pkg of 1, 5 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography

List Price:
 
Inquire
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Nuvia™ IMAC Uncharged Resin, 10 L

12004039
Pkg of 1, 10 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography

List Price:
 
Inquire
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Foresight™ Nuvia™ Ni-Charged IMAC, 1 ml column

12004038
Pkg of 1, 1 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin

List Price:
 
$157.02
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Foresight™ Nuvia™ Ni-Charged IMAC, 5 ml column

12004037
Pkg of 1, 5 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin

List Price:
 
$296.26
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Foresight™ Nuvia™ Ni-Charged IMAC Filter Plates, 20 µl
Foresight™ Nuvia™ Ni-Charged IMAC Filter Plates, 20 µl

12004035
2 x 96-well plates, 20 µl, filter plate prepacked with Nuvia IMAC Affinity Chromatography Resin

List Price:
 
$705.12
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Foresight™ Nuvia™ Ni-Charged IMAC RoboColumn Unit, 200 µl

12004051
1 row of 8 columns, 200 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin

List Price:
 
$285.22
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Foresight™ Nuvia™ Foresight Nuvia Ni-Charged IMAC RoboColumn Unit, 600 µl
Foresight™ Nuvia™ Ni-Charged IMAC RoboColumn Unit, 600 µl

12004052
1 row of 8 columns, 600 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin

List Price:
 
$344.32
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Bio-Scale Mini Nuvia IMAC Cartridges
Bio-Scale™ Mini Nuvia™ IMAC Cartridges

7800811
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system

List Price:
 
$165.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Bio-Scale Mini Nuvia IMAC Cartridges
Bio-Scale™ Mini Nuvia™ IMAC Cartridges

7800812
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system

List Price:
 
$620.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Number Description Options
D116698 Instruction Manual, Nuvia™ IMAC Resin Click to download
6859 Nuvia™ IMAC Affinity Chromatography Resin Product Information Sheet, Ver B Click to download
10041106 Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml, Rev A Click to download
10042131 Instruction Manual, Bio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges, 1 and 5 ml Quick Start Guide, Rev B Click to download
10024831 Instruction Manual, Foresight™ Chromatography Media Columns 1 and 5 ml, Rev A Click to download
10027565 Instruction Manual, Foresight™ RoboColumn Units 200 and 600 µl, Rev A Click to download
10024413 Instruction Manual, Foresight™ Chromatography Media Filter Plates, Rev A Click to download
6297 Foresight™ Prepacked Plates and Columns Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download
6860 A Scalable Immobilized Metal Affinity Chromatography Resin for Process Purification Click to download
6769 From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification (Poster), Ver A Click to download
6964 A Nuvia IMAC Resin–Based Purification Workflow for the Production of Highly Pure Recombinant Proteins Click to download