Nuvia™ IMAC Metal Affinity Resin

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Nuvia IMAC is a metal chelate affinity chromatography resin that contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The inert hydrophilic surface of the base bead and the chemical structure of NTA ensure highly selective binding of recombinant histidine-tagged proteins when charged with Ni²⁺, or with other transition metals such as Zn²⁺ or Cu²⁺. The optimized particle size and high porosity allows operation at increasingly higher flow rates without compromising binding capacity. Nuvia IMAC can be used at any scale from laboratory to manufacturing, providing robust, reproducible, and efficient purifications each time.

Nuvia IMAC Resin is stable in a broad range of chemicals. It is compatible with all reagents traditionally used for histidine-tagged protein purification, including reducing agents, detergents, denaturing agents, and additives.

Features and Benefits

Easy and efficient scale up from lab to manufacturing scale purifications
Optimized particle size for high flow rate and low backpressure
Superior dynamic binding capacity at high flow rates
Withstands repeated clean-in-place/recharging cycles
Robust recovery and reproducibility
Full regulatory support

Applications and Uses of Nuvia IMAC Resin

For purifying biomolecules in a variety of workflows:

Excellent single-step purity via affinity chromatography
Quick, efficient separation of biomolecules
Contaminant removal
Robust base matrix

This IMAC resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.

Product Options

Package Size	Catalog Number
25 ml	7800800
100 ml	7800801
500 ml	7800802
5 L	12003233
10 L	12002782

More Information

Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage. Prepacked Bio-Scale™ Mini Nuvia IMAC Cartridges with Ni-charged NTA are available.

Particle size	38–53 µm
Ligand density	≥ 18 µmol/ml
Dynamic binding capacity*	> 40 mg/ml at 300 cm/hr *10% breakthrough capacity determined with 1.2 mg/ml of a 40 kD histidine-tagged protein in 50 mM sodium phosphate, 5 mM imidazole, 300 mM NaCl (pH 7.5).
Compression factor	1.20–1.25
Recommended linear flow rate range	50–300 cm/hr
Pressure vs. flow performance	Under 2 bar at flow rate of 300 cm/hr in DI water (20 x 20 cm packed bed, 1.2 compression factor)
pH stability	2–14
Shipping solution	2% benzyl alcohol or 20% ethanol
Regeneration	50 mM EDTA, pH 8.0 (Stripping) 1N NaOH (CIP/SIP) 0.1 M Ni₂SO₄ (Recharging)
Cleaning-in-place (CIP) solutions	1N NaOH
Sanitization	1N NaOH
Storage condition	20% Ethanol
Chemical compatibility	Reducing agents (β-ME, TCEP, DTT) Denaturing agents (GnHCl) Detergents (Triton X-100, NP-40, CHAPS, CHAPSO) Additives (glycerol) **No recharge required. Refer to the instruction manual for a complete list.
Chemical stability	48 hr with 1M NaOH, 1 week with 0.01M HCl ***Recharge required. Refer to the instruction manual for a complete list.

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