Nuvia aPrime 4A Media

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Nuvia aPrime 4A Media | Hydrophobic Anion Exchange Resin | Bio-Rad.com

Overview

Effect of Flow Rate on Nuvia aPrime 4A Resin Binding Capacity
Dynamic Binding Capacity Comparison

Effect of flow rate on Nuvia aPrime 4A Resin binding capacity for polyclonal human IgG (hIgG). Column dimension: 1.2 x 8.8 cm; equilibration buffer: 50 mM sodium phosphate, pH 8.0. DBC, dynamic binding capacity.

Pressure/Flow Performance of Nuvia aPrime 4A Media

Pressure-flow performance of Nuvia aPrime 4A Resin. Nuvia aPrime 4A Resin slurry prepared in 1x PBS, pH 7.5 was packed into a 20 x 20 cm column by axial compression at 300 cm/hr with a compression factor of 1.25.

Why Is It Important to Screen Chromatography Resins?

Bio-Rad Chromatography Support Scientist, William Rushton discusses the importance of screening chromatography resins to accelerate method development and optimize downstream purification platforms. Bio-Rad's innovative chromatography resins have produced tangible benefits such as higher process yield, reduced buffer consumption, and processes with fewer unit operations. These resins are available in prepacked formats for the entire purification development cycle, from high-throughput resin screening to small-scale method development, and scale-up optimization.

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Nuvia aPrime 4A Resin

No Biomolecule Too Challenging.

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Nuvia aPrime 4A is a hydrophobic anion exchange resin designed with a distinct balance of modes optimized for biomolecule interactions. The resin's ligand density and hydrophobicity are engineered to facilitate selective and reversible binding of target molecules for high purity and yield. Nuvia aPrime offers a wide design space and straightforward method development to purify even the most complex molecules.

Benefits

  • Broad range of binding and elution conditions for optimal process scale-up
  • Easy parameter manipulation for complex feed stream purification
  • Exceptional clearance of impurities with high target recovery

Purification of Monoclonal Antibody

The Nuvia aPrime 4A Bead is designed to provide a high DBC and excellent recovery of biomolecules. The 50 µm particle size offers good resolution and high productivity while maintaining low column pressure. In this example, the resin to clear high molecular weight species from an acidic mAb (pI ~6.9) feedstream at pH 7.8, Nuvia aPrime 4A interacts with the mAb through both electrostatic and hydrophobic interactions. The mAb monomer was eluted at ~pH 6 with an overall recovery of ~90%. The high molecular weight impurities were retained until the resin was stripped with a pH 4 buffer (Figure 1).


Fig.1. 90% recovery of mAb S with Nuvia aPrime 4A Resin.
A solution sample containing (about 10 mg of target mAb in 20 mM NaPO4, pH 7.8) was injected onto a 1 ml column and eluted by buffers at pH 6. Clear separation of the impurities from the monomeric fraction was observed. AU, absorbance units.


Straightforward Method Development

A design of experiment can be used to optimize process conditions. In the example below, method development was tested for flow-through purification of a basic protein with a pI of ~8.45. The flowthrough showed excellent clearance of impurities as determined by the monomer content (Figure 2) while providing good monomer recovery (Figure 3).



Fig 2. Excellent clearance of impurities

Binding buffer pH and NaCl affect monomer content in the flow-through fraction to yield good clearance


Fig 3. High recovery
Binding buffer pH and NaCl yield high monomer recovery in the flow-through fraction.


Product Formats

Product Catalog Number
Nuvia aPrime 4A Media, 25 ml 12007397
Nuvia aPrime 4A Media, 100 ml 12007396
Nuvia aPrime 4A Media, 500 ml 12007379
Nuvia aPrime 4A Media, 5 L 12007380
Nuvia aPrime 4A Media, 10 L 12007391

Coming SoonScreening Tools

  • Foresight CHT XT Column, 1 ml

    Foresight Nuvia aPrime 4A Column, 1 ml

    12007392

  • Foresight CHT XT Column, 5 ml

    Foresight Nuvia aPrime 4A Column, 5 ml

    12007393

  • Foresight CHT XT, 200 μl Robocolumn

    Foresight Nuvia aPrime 4A RoboColumn Unit, 200 μl

    12007394

  • Foresight CHT XT, 600 μl Robocolumn

    Foresight Nuvia aPrime 4A RoboColumn Unit, 600 μl

    12007395

  • Foresight CHT XT, 20 μl Plates

    Foresight Nuvia aPrime 4A Plates, 20 μl

    12007411

More Information

Base matrix composition Macroporous highly crosslinked polymer
Functional group Aromatic hydrophobic anion exchanger
Median particle size 50 ± 10 µm
Ligand density 100 ± 20 µeq/ml
Dynamic binding capacity* ≥50 mg/ml at 300 cm/hr
Recommended linear flow rate 50–300 cm/hr
Pressure vs. flow performance** Under 3 bar at flow rate of 300 cm/hr
Compression factor 1.1–1.25
Shipping solution 20% ethanol + 1 M NaCl
pH stability*** Short term: 2–14
Regeneration 1 N NaOH
Sanitization 1 N NaOH
Storage condition 20% ethanol
Chemical stability 1 N NaOH, 1 M HCl, 25% acetic acid, 8 M urea, 6 M guanidine HCl, 3 M NaCl, 1% Triton X-100, 20–70% ethanol, 30% isopropanol
Shelf life†† 5 years
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Nuvia aPrime 4A Media, 25 ml

12007397
25 ml, hydrophobic anion exchange resin, 50 µm particle size

12007397 templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007397_1549474856.xml 12007397 02/06/19 05:30 PM en_US true internet/product_SKU 2019-05-04 09:54:05 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007397_1549474856.xml /default/main/bio-rad/WORKAREA/default jrtv5axy 2019-02-06 17:40:56 true jrtv5axy templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007397_1549474856.xml PSD N /default/main/bio-rad en 02/06/29 06:39 AM 1555966055269 2019-05-04 09:54:05 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007397_1549474856.xml 12007397-nuvia-aprime-4a-media-25-ml 1 12007397 Nuvia aPrime 4A Media 25 ml Nuvia aPrime 4A Media, 25 ml 25 ml, hydrophobic anion exchange resin, 50 &micro;m particle size 25 ml, hydrophobic anion exchange resin, 50 &micro;m particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/12007397-tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/12007397-view.jpg preview ,VN Jessica Low Nuvia aPrime 4A Media, 25 ml 25 ml, hydrophobic anion exchange resin, 50 µm particle size Hydrophobic anion exchange resin, Mixed-mode resin, multi modal resin, mAbs, monoclonal antibodies, bispecifics, fragments 02/06/19 05:30 PM 02/06/29 06:39 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN PSD /PSD/Ion-Exchange-Media- N 0 <p>Nuvia aPrime 4A is a hydrophobic anion exchange resin designed to provide high efficiency and productivity for process-scale purification of a wide variety of therapeutic proteins. It can be used for both capture and polish purification of biomolecules that are difficult to purify. Nuvia aPrime resin&rsquo;s mixed-mode ligand structure provides the unique advantage of an optimal balance between high target yield and purity.</p> <h2 class="descHeader">Benefits</h2> <ul> <li>Broad range of binding and elution conditions for optimal process scale-up</li> <li>Easy parameter manipulation for complex feed stream purification</li> <li>Exceptional clearance of impurities with high target recovery</li> </ul> <h2 class="descHeader">Product&nbsp;Formats</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Product</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 25 ml</td> <td valign="middle">12007397</td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 100 ml</td> <td valign="middle"><a href="/en-us/sku/12007396-nuvia-aprime-4a-media-100-ml?ID=12007396">12007396</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 500 ml</td> <td valign="middle"><a href="/en-us/sku/12007379-nuvia-aprime-4a-media-500-ml?ID=12007379">12007379</a></td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 5 L</td> <td valign="middle"><a href="/en-us/sku/12007380-nuvia-aprime-4a-media-5-l?ID=12007380">12007380</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 10 L</td> <td valign="middle"><a href="/en-us/sku/12007391-nuvia-aprime-4a-media-10-l?ID=12007391">12007391</a></td> </tr> </tbody> </table> <div class="corner-box"> <h3><span class="soon-txt">Coming Soon</span>Screening Tools</h3> <ul class="list-set list-five-col top-margin"> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003150.jpg" alt="Foresight CHT XT Column, 1 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 1 ml</h3> <p>12007392</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003149.jpg" alt="Foresight CHT XT Column, 5 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 5 ml</h3> <p>12007393</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003152.jpg" alt="Foresight CHT XT, 200 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit, 200 &mu;l</h3> <p>12007394</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003148.jpg" alt="Foresight CHT XT, 600 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit,&nbsp;600&nbsp;&mu;l</h3> <p>12007395</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003151.jpg" alt="Foresight CHT XT, 20 &mu;l Plates" /> <h3>Foresight Nuvia aPrime 4A Plates, 20 &mu;l</h3> <p>12007411</p> </li> </ul> </div> <h2 class="descHeader">More Information</h2> <ul> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/Bulletin_7142.pdf" target="_blank" rel="noopener noreferrer">Information Sheet</a></li> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/10000098359.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global 12007397.html /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global/12007397.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007397&CartQuote=QT&quantity= 1298 false true false $250.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007397&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007397&quantity= 12007397
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Nuvia aPrime 4A Media, 100 ml

12007396
100 ml, hydrophobic anion exchange resin, 50 µm particle size

12007396 templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007396_1549499428.xml 12007396 02/07/19 12:23 AM en_US true internet/product_SKU 2019-05-04 09:53:47 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007396_1549499428.xml /default/main/bio-rad/WORKAREA/default jrtv5axv 2019-02-07 00:30:28 true jrtv5axv templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007396_1549499428.xml PSD N /default/main/bio-rad en 02/06/29 09:13 AM 1555966055226 2019-05-04 09:53:47 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007396_1549499428.xml 12007396-nuvia-aprime-4a-media-100-ml 1 12007396 Nuvia aPrime 4A Media 100 ml Nuvia aPrime 4A Media, 100 ml 100 ml, hydrophobic anion exchange resin, 50 &micro;m particle size 100 ml, hydrophobic anion exchange resin, 50 &micro;m particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/12007396-tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/12007396-view.jpg preview ,VN Jessica Low Nuvia aPrime 4A Media, 100 ml 100 ml, hydrophobic anion exchange resin, 50 µm particle size Hydrophobic anion exchange resin, Mixed-mode resin, multi modal resin, mAbs, monoclonal antibodies, bispecifics, fragments, 12007396 02/07/19 12:23 AM 02/06/29 09:13 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN PSD /PSD/Ion-Exchange-Media- N 0 <p>Nuvia aPrime 4A is a hydrophobic anion exchange resin designed to provide high efficiency and productivity for process-scale purification of a wide variety of therapeutic proteins. It can be used for both capture and polish purification of biomolecules that are difficult to purify. Nuvia aPrime resin&rsquo;s mixed-mode ligand structure provides the unique advantage of an optimal balance between high target yield and purity.</p> <h2 class="descHeader">Benefits</h2> <ul> <li>Broad range of binding and elution conditions for optimal process scale-up</li> <li>Easy parameter manipulation for complex feed stream purification</li> <li>Exceptional clearance of impurities with high target recovery</li> </ul> <h2 class="descHeader">Product&nbsp;Formats</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Product</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 25 ml</td> <td valign="middle"><a href="/en-us/sku/12007397-nuvia-aprime-4a-media-25-ml?ID=12007397">12007397</a></td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 100 ml</td> <td valign="middle">12007396</td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 500 ml</td> <td valign="middle"><a href="/en-us/sku/12007379-nuvia-aprime-4a-media-500-ml?ID=12007379">12007379</a></td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 5 L</td> <td valign="middle"><a href="/en-us/sku/12007380-nuvia-aprime-4a-media-5-l?ID=12007380">12007380</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 10 L</td> <td valign="middle"><a href="/en-us/sku/12007391-nuvia-aprime-4a-media-10-l?ID=12007391">12007391</a></td> </tr> </tbody> </table> <div class="corner-box"> <h3><span class="soon-txt">Coming Soon</span>Screening Tools</h3> <ul class="list-set list-five-col top-margin"> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003150.jpg" alt="Foresight CHT XT Column, 1 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 1 ml</h3> <p>12007392</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003149.jpg" alt="Foresight CHT XT Column, 5 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 5 ml</h3> <p>12007393</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003152.jpg" alt="Foresight CHT XT, 200 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit, 200 &mu;l</h3> <p>12007394</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003148.jpg" alt="Foresight CHT XT, 600 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit,&nbsp;600&nbsp;&mu;l</h3> <p>12007395</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003151.jpg" alt="Foresight CHT XT, 20 &mu;l Plates" /> <h3>Foresight Nuvia aPrime 4A Plates, 20 &mu;l</h3> <p>12007411</p> </li> </ul> </div> <h2 class="descHeader">More Information</h2> <ul> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/Bulletin_7142.pdf" target="_blank" rel="noopener noreferrer">Information Sheet</a></li> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/10000098359.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global 12007396.html /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global/12007396.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007396&CartQuote=QT&quantity= 1298 false true false $675.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007396&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007396&quantity= 12007396
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Nuvia aPrime 4A Media, 500 ml

12007379
500 ml, hydrophobic anion exchange resin, 50 µm particle size

12007379 templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007379_1549487946.xml 12007379 02/06/19 09:12 PM en_US true internet/product_SKU 2019-05-04 10:09:28 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007379_1549487946.xml /default/main/bio-rad/WORKAREA/default jrtv5axs 2019-02-06 21:19:07 true jrtv5axs templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007379_1549487946.xml PSD N /default/main/bio-rad en 02/06/29 09:13 AM 1555966054957 2019-05-04 10:09:28 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007379_1549487946.xml 12007379-nuvia-aprime-4a-media-500-ml 1 12007379 Nuvia aPrime 4A Media 500 ml Nuvia aPrime 4A Media, 500 ml 500 ml, hydrophobic anion exchange resin, 50 µm particle size 500 ml, hydrophobic anion exchange resin, 50 µm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/12007379-tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/12007379-view.jpg preview ,VN Jessica Low Nuvia aPrime 4A Media, 500 ml 500 ml, hydrophobic anion exchange resin, 50 µm particle size Hydrophobic anion exchange resin, Mixed-mode resin, multi modal resin, mAbs, monoclonal antibodies, bispecifics, fragments, 12007379 02/06/19 09:12 PM 02/06/29 09:13 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN PSD /PSD/Ion-Exchange-Media- N 0 <p>Nuvia aPrime 4A is a hydrophobic anion exchange resin designed to provide high efficiency and productivity for process-scale purification of a wide variety of therapeutic proteins. It can be used for both capture and polish purification of biomolecules that are difficult to purify. Nuvia aPrime resin&rsquo;s mixed-mode ligand structure provides the unique advantage of an optimal balance between high target yield and purity.</p> <h2 class="descHeader">Benefits</h2> <ul> <li>Broad range of binding and elution conditions for optimal process scale-up</li> <li>Easy parameter manipulation for complex feed stream purification</li> <li>Exceptional clearance of impurities with high target recovery</li> </ul> <h2 class="descHeader">Product&nbsp;Formats</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Product</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 25 ml</td> <td valign="middle"><a href="/en-us/sku/12007397-nuvia-aprime-4a-media-25-ml?ID=12007397">12007397</a></td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 100 ml</td> <td valign="middle"><a href="/en-us/sku/12007396-nuvia-aprime-4a-media-100-ml?ID=12007396">12007396</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 500 ml</td> <td valign="middle">12007379</td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 5 L</td> <td valign="middle"><a href="/en-us/sku/12007380-nuvia-aprime-4a-media-5-l?ID=12007380">12007380</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 10 L</td> <td valign="middle"><a href="/en-us/sku/12007391-nuvia-aprime-4a-media-10-l?ID=12007391">12007391</a></td> </tr> </tbody> </table> <div class="corner-box"> <h3><span class="soon-txt">Coming Soon</span>Screening Tools</h3> <ul class="list-set list-five-col top-margin"> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003150.jpg" alt="Foresight CHT XT Column, 1 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 1 ml</h3> <p>12007392</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003149.jpg" alt="Foresight CHT XT Column, 5 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 5 ml</h3> <p>12007393</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003152.jpg" alt="Foresight CHT XT, 200 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit, 200 &mu;l</h3> <p>12007394</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003148.jpg" alt="Foresight CHT XT, 600 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit,&nbsp;600&nbsp;&mu;l</h3> <p>12007395</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003151.jpg" alt="Foresight CHT XT, 20 &mu;l Plates" /> <h3>Foresight Nuvia aPrime 4A Plates, 20 &mu;l</h3> <p>12007411</p> </li> </ul> </div> <h2 class="descHeader">More Information</h2> <ul> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/Bulletin_7142.pdf">Information Sheet</a></li> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/10000098359.pdf">Instruction Manual</a></li> </ul> /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global 12007379.html /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global/12007379.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007379&CartQuote=QT&quantity= 1298 false true false $2,200.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007379&AddToDefaultWishList=true&quantity= false false true 41105300 true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007379&quantity= 12007379
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Nuvia aPrime 4A Media, 5 L

12007380
5 L, hydrophobic anion exchange resin, 50 µm particle size

12007380 templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007380_1549488192.xml 12007380 02/06/19 09:19 PM en_US true internet/product_SKU 2019-05-04 10:09:21 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007380_1549488192.xml /default/main/bio-rad/WORKAREA/default jrtv5axt 2019-02-06 21:23:13 true jrtv5axt templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007380_1549488192.xml PSD N /default/main/bio-rad en 02/06/29 09:13 AM 1555966055002 2019-05-04 10:09:21 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007380_1549488192.xml 12007380-nuvia-aprime-4a-media-5-l 1 12007380 Nuvia aPrime 4A Media 5 L Nuvia aPrime 4A Media, 5 L 5 L, hydrophobic anion exchange resin, 50 µm particle size 5 L, hydrophobic anion exchange resin, 50 µm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/12007380-tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/12007380-view.jpg preview ,VN Jessica Low Nuvia aPrime 4A Media, 5 L 5 L, hydrophobic anion exchange resin , 50 µm particle size Hydrophobic anion exchange resin, Mixed-mode resin, multi modal resin, mAbs, monoclonal antibodies, bispecifics, fragments, 12007380 02/06/19 09:19 PM 02/06/29 09:13 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN PSD /PSD/Ion-Exchange-Media- N 0 <p>Nuvia aPrime 4A is a hydrophobic anion exchange resin designed to provide high efficiency and productivity for process-scale purification of a wide variety of therapeutic proteins. It can be used for both capture and polish purification of biomolecules that are difficult to purify. Nuvia aPrime resin&rsquo;s mixed-mode ligand structure provides the unique advantage of an optimal balance between high target yield and purity.</p> <h2 class="descHeader">Benefits</h2> <ul> <li>Broad range of binding and elution conditions for optimal process scale-up</li> <li>Easy parameter manipulation for complex feed stream purification</li> <li>Exceptional clearance of impurities with high target recovery</li> </ul> <h2 class="descHeader">Product&nbsp;Formats</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Product</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 25 ml</td> <td valign="middle"><a href="/en-us/sku/12007397-nuvia-aprime-4a-media-25-ml?ID=12007397">12007397</a></td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 100 ml</td> <td valign="middle"><a href="/en-us/sku/12007396-nuvia-aprime-4a-media-100-ml?ID=12007396">12007396</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 500 ml</td> <td valign="middle"><a href="/en-us/sku/12007379-nuvia-aprime-4a-media-500-ml?ID=12007379">12007379</a></td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 5 L</td> <td valign="middle">12007380</td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 10 L</td> <td valign="middle"><a href="/en-us/sku/12007391-nuvia-aprime-4a-media-10-l?ID=12007391">12007391</a></td> </tr> </tbody> </table> <div class="corner-box"> <h3><span class="soon-txt">Coming Soon</span>Screening Tools</h3> <ul class="list-set list-five-col top-margin"> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003150.jpg" alt="Foresight CHT XT Column, 1 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 1 ml</h3> <p>12007392</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003149.jpg" alt="Foresight CHT XT Column, 5 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 5 ml</h3> <p>12007393</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003152.jpg" alt="Foresight CHT XT, 200 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit, 200 &mu;l</h3> <p>12007394</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003148.jpg" alt="Foresight CHT XT, 600 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit,&nbsp;600&nbsp;&mu;l</h3> <p>12007395</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003151.jpg" alt="Foresight CHT XT, 20 &mu;l Plates" /> <h3>Foresight Nuvia aPrime 4A Plates, 20 &mu;l</h3> <p>12007411</p> </li> </ul> </div> <h2 class="descHeader">More Information</h2> <ul> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/Bulletin_7142.pdf" target="_blank" rel="noopener noreferrer">Information Sheet</a></li> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/10000098359.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global 12007380.html /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global/12007380.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007380&CartQuote=QT&quantity= 1298 false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007380&AddToDefaultWishList=true&quantity= false false true 41105300 true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007380&quantity= 12007380
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Nuvia aPrime 4A Media, 10 L

12007391
10 L, hydrophobic anion exchange resin, 50 µm particle size

12007391 templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007391_1549488487.xml 12007391 02/06/19 09:24 PM en_US true internet/product_SKU 2019-05-04 10:09:22 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007391_1549488487.xml /default/main/bio-rad/WORKAREA/default jrtv5axw 2019-02-06 21:28:07 true jrtv5axw templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007391_1549488487.xml PSD N /default/main/bio-rad en 02/06/29 09:13 AM 1555966055046 2019-05-04 10:09:22 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/PSD/Ion-Exchange-Media-/12007391_1549488487.xml 12007391-nuvia-aprime-4a-media-10-l 1 12007391 Nuvia aPrime 4A Media 10 L Nuvia aPrime 4A Media, 10 L 10 L, hydrophobic anion exchange resin, 50 µm particle size 10 L, hydrophobic anion exchange resin, 50 µm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/12007391-tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/12007391-view.jpg preview ,VN Jessica Low Nuvia aPrime 4A Media, 10 L 10 L, hydrophobic anion exchange resin, 50 µm particle size Hydrophobic Anion Exchange resin, Mixed-mode resin, multi modal resin, 12007391 02/06/19 09:24 PM 02/06/29 09:13 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN PSD /PSD/Ion-Exchange-Media- N 0 <p>Nuvia aPrime 4A is a hydrophobic anion exchange resin designed to provide high efficiency and productivity for process-scale purification of a wide variety of therapeutic proteins. It can be used for both capture and polish purification of biomolecules that are difficult to purify. Nuvia aPrime resin&rsquo;s mixed-mode ligand structure provides the unique advantage of an optimal balance between high target yield and purity.</p> <h2 class="descHeader">Benefits</h2> <ul> <li>Broad range of binding and elution conditions for optimal process scale-up</li> <li>Easy parameter manipulation for complex feed stream purification</li> <li>Exceptional clearance of impurities with high target recovery</li> </ul> <h2 class="descHeader">Product&nbsp;Formats</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Product</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 25 ml</td> <td valign="middle"><a href="/en-us/sku/12007397-nuvia-aprime-4a-media-25-ml?ID=12007397">12007397</a></td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 100 ml</td> <td valign="middle"><a href="/en-us/sku/12007396-nuvia-aprime-4a-media-100-ml?ID=12007396">12007396</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 500 ml</td> <td valign="middle"><a href="/en-us/sku/12007379-nuvia-aprime-4a-media-500-ml?ID=12007379">12007379</a></td> </tr> <tr> <td valign="middle">Nuvia aPrime 4A Media, 5 L</td> <td valign="middle"><a href="/en-us/sku/12007380-nuvia-aprime-4a-media-5-l?ID=12007380">12007380</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">Nuvia aPrime 4A Media, 10 L</td> <td valign="middle">12007391</td> </tr> </tbody> </table> <div class="corner-box"> <h3><span class="soon-txt">Coming Soon</span>Screening Tools</h3> <ul class="list-set list-five-col top-margin"> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003150.jpg" alt="Foresight CHT XT Column, 1 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 1 ml</h3> <p>12007392</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003149.jpg" alt="Foresight CHT XT Column, 5 ml" /> <h3>Foresight Nuvia aPrime 4A Column, 5 ml</h3> <p>12007393</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003152.jpg" alt="Foresight CHT XT, 200 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit, 200 &mu;l</h3> <p>12007394</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003148.jpg" alt="Foresight CHT XT, 600 &mu;l Robocolumn" /> <h3>Foresight Nuvia aPrime 4A RoboColumn Unit,&nbsp;600&nbsp;&mu;l</h3> <p>12007395</p> </li> <li><img src="/webroot/web/images/ps/products/multimodal_media/sku_view/global/soon-th_12003151.jpg" alt="Foresight CHT XT, 20 &mu;l Plates" /> <h3>Foresight Nuvia aPrime 4A Plates, 20 &mu;l</h3> <p>12007411</p> </li> </ul> </div> <h2 class="descHeader">More Information</h2> <ul> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/Bulletin_7142.pdf" target="_blank" rel="noopener noreferrer">Information Sheet</a></li> <li>Nuvia aPrime 4A Media <a href="/webroot/web/pdf/ps/literature/10000098359.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global 12007391.html /webroot/web/html/ps/global/english/products/ion_exchange_media/product_detail/description/global/12007391.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007391&CartQuote=QT&quantity= 1298 false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007391&AddToDefaultWishList=true&quantity= false false true 41105300 true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12007391&quantity= 12007391
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Number Description Options
10000098359 Instruction Manual, Nuvia aPrime 4A Hydrophobic Anion Exchange Resin Click to download
7142 Nuvia aPrime 4A Media Information Sheet Click to download
7135 Maximize Monomer Purity and Recovery during mAb Purification Click to download
7136 Get the Best Monomer Recovery during Protein Purification Click to download

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