Nucleic Acid Extraction Module

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Nucleic Acid Extraction Module

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 12 workstations, or 25 genomic DNA extractions
Lysis buffer, 20 ml 1
Dithiothreitol (DTT), 0.3 g 1
Wash buffer, 20 ml 1
Sterile water, 2.5 ml 1
Micropestles 25
Spin columns 25
Microcentrifuge tubes, 2.0 ml 25
Microcentrifuge tubes, 1.5 ml 30
Colored microcentrifuge tubes, 2.0 ml 60
Instruction manual 1
Required Accessories Not Included in Kit:
Plant samples
95–100% lab grade ethanol
Adjustable micropipets
2–20 µl 1
20–200 µl 1–12
100–1,000 µl 1–12
Pipet tips, aerosol barrier
2–20 µl, Xcluda™ B 1 box
20–200 µl, Xcluda D 1 box
100–1,000 µl, Xcluda E 1 box
Microcentrifuges capable of greater than 12,000 x g 1–4
Water bath 1
Heat block 1
Recommended (Optional) Accessories:
Vortexer
Spectrophotometer and cuvettes
Fluorometer (VersaFluor™)
DEPC-treated water, 100 ml
Refills Available Separately:
Nucleic Acid Extraction Refill Kit, includes DTT,
lysis buffer, wash buffer, sterile water
Cloning and Sequencing Explorer Series Overview
Cloning and Sequencing Explorer Series Overview

Prepare your students for the real world of scientific research. Engage them with the opportunity to perform novel, relevant research that can actually contribute to scientific knowledge. In this unique modular lab series, students are guided through an innovative research workflow identical to those performed in genomics labs worldwide. Over a multiple-week lab course, students will combine traditional and cutting edge molecular biology techniques and bioinformatics to clone, sequence, and analyze a housekeeping gene from a plant of your choice ensuring each class produces unique and novel data. The real thing. As a research and diagnostics manufacturing company, Bio-Rad brings unique strengths to the education market, including credibility, high quality products (kits and equipment that work), novel inquiry-based curricula, cutting edge kit applications, competitive pricing, superior teacher professional development programs, long-term strategic partners and allies, and strong personal endorsements from customers across all levels of education.

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To clone a gene, genomic DNA (gDNA) must first be extracted from the organism. The nucleic acid extraction module allows students to extract all nucleic acids from plant cells using tissue grinding and lysis, followed by purification using column chromatography. The final sample, which contains gDNA and RNA, can be used for PCR. RNA can then be removed from the sample by treatment with RNase I as an optional activity.

The protocol provided with this module has been optimized for genomic DNA extraction from most plant species. Different plants yield different quantities of genomic DNA. Each extraction yields sufficient DNA for 16 PCR amplifications.

If you want students to use the nucleic acid extraction module to extract RNA, genomic DNA can be removed from the sample with DNase I treatment. For further RNA work, elution using DEPC-treated water is recommended.

Kit contains sufficient materials for 25 genomic DNA extractions  
Lysis buffer 20 ml
Dithiothreitol (DTT) 0.3 g
Wash buffer 20 ml
Sterile water 2.5 ml
Micropestles 25
Spin columns 25
Microcentrifuge tubes 2.0 ml, 25
Microcentrifuge tubes 1.5 ml, 30
Colored microcentrifuge tubes 2.0 ml, 60
Instruction manual 1
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Nucleic Acid Extraction Module

1665005EDU
First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes

0 1665005EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5005EDU_0921121012468.xml 1665005EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jc99dsnq 2017-11-14 07:07:14 true true internet/product_SKU jc99dsnq templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5005EDU_0921121012468.xml LSE 2019-05-04 11:12:59 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972631727 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:12:59 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5005EDU_0921121012468.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5005EDU_0921121012468.xml 1665005edu-nucleic-acid-extraction-module 1665005EDU Nucleic Acid Extraction Module Nucleic Acid Extraction Module First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5005EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5005EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5005EDU_feature.jpg Nucleic Acid Extraction Module #166-5005EDU RepEx Nucleic Acid Extraction Module #166-5005EDU First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes 1665005, 1665005EDU, nucleic, acid, extraction, module, first, modules, cloning, and, sequencing, explorer, series, removal, plant, genomic, dna, purification, column, chromatography, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 ,VN <p>The first module in Cloning and Sequencing Explorer Series, the Nucleic Acid Extraction Module allows students to extract plant nucleic acids and purify them by column chormatography. The module includes buffers, DTT, columns, microcentrifuge tubes, micropestles, and instruction manual. Reagents are sufficient for 25 genomic DNA extractions. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-5005EDU_0921121012468_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-5005EDU_0921121012468_en.html 1140 <b>Kit contains sufficient materials for 25 genomic DNA extractions</b> &nbsp; 1 0 2684 Lysis buffer, 20 ml 1 1 0 2489 Dithiothreitol (DTT), 0.3 g 1 1 0 2685 Wash buffer, 20 ml 1 1 0 2394 Sterile water, 2.5 ml 1 1 0 2404 Micropestles 25 1 0 2405 Spin columns 25 1 0 2686 Microcentrifuge tubes, 2.0 ml 25 1 0 2687 Microcentriuge tubes, 1.5 ml 30 1 0 2421 Colored microcentrifuge tubes, 2.0 ml 60 1 0 2398 Curriculum, including teacher's guide, student manual, and graphic quick guide 1 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665005EDU&CartQuote=QT&quantity= 1711 false true false $215.00 true %24172.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665005EDU&AddToDefaultWishList=true&quantity= true false true 41105500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665005EDU&quantity= 1665005EDU
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Nucleic Acid Extraction Refill Kit

1665006EDU
Consumables refill kit for use with Nucleic Acid Extraction module (#166-5005EDU), contains DTT, lysis buffer, wash buffer, sterile water; education use only

1665006EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Nucleic-Acid-Extraction-Module/166-5006EDU_0921121013551.xml 0 1665006EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jcrucwxm 2017-11-14 07:07:15 true true internet/product_SKU jcrucwxm templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Nucleic-Acid-Extraction-Module/166-5006EDU_0921121013551.xml LSE 2019-05-04 11:12:27 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972632778 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:12:27 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Nucleic-Acid-Extraction-Module/166-5006EDU_0921121013551.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Nucleic-Acid-Extraction-Module/166-5006EDU_0921121013551.xml 1665006edu-nucleic-acid-extraction-refill-kit 1665006EDU Nucleic Acid Extraction Refill Kit Nucleic Acid Extraction Refill Kit Consumables refill kit for use with Nucleic Acid Extraction module (#166-5005EDU), contains DTT, lysis buffer, wash buffer, sterile water; education use only Consumables refill kit for use with Nucleic Acid Extraction module (#166-5005EDU), contains DTT, lysis buffer, wash buffer, sterile water; education use only Consumables refill kit for use with Nucleic Acid Extraction module (#166-5005EDU), contains DTT, lysis buffer, wash buffer, sterile water; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5006EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5006EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5006EDU_feature.jpg RepEx 166-5006EDU Consumables refill kit for use with Nucleic Acid Extraction module (#166-5005EDU), contains DTT, lysis buffer, wash buffer, sterile water; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Nucleic-Acid-Extraction-Module N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665006EDU&CartQuote=QT&quantity= 1711 false true false $140.00 true %24112.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665006EDU&AddToDefaultWishList=true&quantity= true false true 41105500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665006EDU&quantity= 1665006EDU
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1665007 Instruction Manual, Nucleic Acid Extraction Module, Biotechnology Explorer, Rev A Please login to download this document

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