Nuclear Staining Dyes

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PureBlu™ Nuclear Staining Dyes

Overview

Available Nuclear Staining Dyes
PureBlu™ Nuclear
Staining Dye
Excitation
Maximum, nm
Emission
Maximum, nm
Optimal Channel on
ZOE™ Cell Imager
Catalog
Number
DAPI 359 461 Blue 135-1303
Hoechst 33342 350 461 Blue 135-1304

 
PureBlu™ Nuclear Staining Dye One-Step Dilution

PureBlu Nuclear Staining Dye One-Step Dilution

Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to 50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM); PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)).
 
Nuclear Staining Dyes Viewed with the ZOE™ Fluorescent Cell Imager

HeLa cells transfected with a GFP-expressing plasmid were counterstained with PureBlu™ DAPI or Hoechst 33342 Nuclear Staining Dyes and then imaged in a 6-well plate with the ZOE™ Fluorescent Cell Imager.

Nuclear Staining dyes

A, GFP-expressing HeLa cells; B, counterstained with PureBlu™ DAPI Nuclear Staining Dye; C, merged view: GFP + PureBlu DAPI staining.
 

Nuclear Staining dyes

A, GFP-expressing HeLa cells; B, counterstained with PureBlu™ Hoechst 33342 Nuclear Staining Dye; C, merged view: GFP + PureBlu Hoechst 33342 staining.


 
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PureBlu™ Nuclear Staining Dyes are designed to specifically stain the nuclei of cells in fixed and unfixed samples for fluorescence microscopy and cell imaging applications.

Based on the well-recognized DAPI and Hoechst 33342 chemistries, PureBlu Dyes are offered in a ready-to-reconstitute, high-purity powder format. PureBlu DAPI and PureBlu Hoechst 33342 Dyes are both ready to use after a single dilution — no weighing required.

PureBlu Nuclear Staining Dye One-Step Dilution

Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to
50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM);
PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)).
 

Features and Benefits of PureBlu Nuclear Staining Dyes

  • High purity formulation for quality results in challenging experiments
  • Pre-aliquoted powder format eliminates time-consuming weighing steps
  • Convenient format allows you to generate working solution with only one dilution step after resuspension
  • Compatible with multicolor experiments

Applications and Uses of PureBlu Nuclear Staining Dyes

  • Nuclear staining
  • Microscopy and cell imaging
  • Multicolor experiments

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PureBlu™ DAPI Nuclear Staining Dye

1351303
Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder

1351303 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1303_1403214125.xml 1351303 06/19/14 02:26 PM en_US true internet/product_SKU 2018-02-09 18:07:07 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1303_1403214125.xml /default/main/bio-rad/WORKAREA/default jb42cv1q 2017-11-14 07:07:54 true jb42cv1q templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1303_1403214125.xml LSR N /default/main/bio-rad en 06/19/24 02:33 PM 1513987486216 2018-02-09 18:07:07 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1303_1403214125.xml 1351303-pureblu-dapi-nuclear-staining-dye 1 1351303 PureBluDAPINuclearStainingDye PureBlu™ DAPI Nuclear Staining Dye Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder /webroot/web/images/lsr/products/cell_imaging/sku_thumb/global/pureblu-dapi-nuclear-staining-dye-sku-135-1303-thumb.jpg /webroot/web/images/lsr/products/cell_imaging/sku_feature/global/pureblu-dapi-nuclear-staining-dye-sku-135-1303-detail.jpg PureBlu DAPI Nuclear Staining Dye preview tommy a PureBlu™ DAPI Nuclear Staining Dye Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder 1351303, pureblu, dapi, nuclear, staining, dye, powder, cell, nucleus, stain, fluorescence, microscopy, imaging 06/19/14 02:26 PM 06/19/24 02:33 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Cell-Imaging/Nuclear-Staining-Dyes N 0 <p>Use PureBlu DAPI Dye for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu DAPI Dye is a highly pure formulation of DAPI in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.</p> <p>DAPI (4',6-diamidino-2-phenylindole) is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. DAPI permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).</p> <p><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/molecular-binding-dapi.jpg" alt="DAPI binding dsDNA" /></p> <p class="caption"><strong>Fig. 1. DAPI binding dsDNA.</strong><br />&nbsp;</p> <p>When bound to dsDNA, PureBlu DAPI Dye has a maximum excitation wavelength in the ultraviolet range (359 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for DAPI, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.</p> <p><br /><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/dapi-emission-spectrum.gif" alt="DAPI emission spectrum" /></p> <p class="caption"><strong>Fig. 2. DAPI excitation and emission spectra.</strong><br />&nbsp;</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/zoe-fluorescent-cell-imager">ZOE&trade; Fluorescent Cell Imaging System</a></li> <li><a href="/en-us/product/tc20-automated-cell-counter?ID=M7FBG34VY">TC20&trade; Automated Cell Counter</a></li> </ul> <h2 class="descHeader">More Information</h2> <p><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10043282.pdf" target="_blank" rel="noopener noreferrer">PureBlu&trade; DAPI Nuclear Staining Dye Product Insert</a></p> /webroot/web/html/lsr/products/SKUs 135-1303.html /webroot/web/html/lsr/products/SKUs/135-1303.html Molecular formula 2963 C<sub>16</sub>H<sub>15</sub>N<sub>5</sub>·2HCl 1 0 Molecular weight 2964 350.25 1 0 Excitation/emission wavelengths 2897 359/461 nm 1 0 CAS Number 2961 28718-90-3 1 0 Purity 2036 >95% (HPLC purified) 1 0 Solubility 2962 Soluble in water and dimethyl sulfoxide (DMSO) 1 0 Storage and use 2877 Long-term storage at –20ºC; protect from light 1 0 Temperature stability 2251 Stable for 2 years at –20°C <br />After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C 1 0 1351304 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml 1450031 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/ZOE-Fluorescent-Cell-Imager/145-0031_1403030204.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351303&CartQuote=QT&quantity= 1108 false true false $113.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351303&AddToDefaultWishList=true&quantity= true false true 12171500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351303&quantity= 1351303
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PureBlu™ Hoechst 33342 Nuclear Staining Dye

1351304
Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder

1351304 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml 1351304 06/19/14 02:49 PM en_US true internet/product_SKU 2018-02-09 18:07:07 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml /default/main/bio-rad/WORKAREA/default jb42cv29 2017-11-14 07:07:54 true jb42cv29 templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml LSR N /default/main/bio-rad en 06/19/24 02:53 PM 1513987486259 2018-02-09 18:07:07 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml 1351304-pureblu-hoechst-33342-nuclear-staining-dye 1 1351304 PureBluHoechst33342NuclearStainDye PureBlu™ Hoechst 33342 Nuclear Staining Dye Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder /webroot/web/images/lsr/products/cell_imaging/sku_thumb/global/pureblu-hoechst-33342-nuclear-staining-dye-sku-135-1304-thumb.jpg /webroot/web/images/lsr/products/cell_imaging/sku_feature/global/pureblu-hoechst-33342-nuclear-staining-dye-sku-135-1304-detail.jpg PureBlu Hoechst 33342 Nuclear Staining Dye preview tommy a PureBlu™ Hoechst 33342 Nuclear Staining Dye 1351304, pureblu, hoechst, 33342, nuclear, staining, dye, powder, cell, nucleus, stain, fluorescence, microscopy, imaging 06/19/14 02:49 PM 06/19/24 02:53 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Cell-Imaging/Nuclear-Staining-Dyes N 0 <p>Use PureBlu Hoechst 33342 Dye for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu Hoechst 33342 Dye is a highly pure formulation of Hoechst 33342 in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.</p> <p>Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).</p> <p><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/molecular-binding-hoechst.jpg" alt="Hoechst 33342 binding dsDNA" /></p> <p class="caption"><strong>Fig. 1. Hoechst 33342 binding dsDNA.</strong><br />&nbsp;</p> <p>When bound to dsDNA, Hoechst 33342 has a maximum excitation wavelength in the ultraviolet range (350 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for Hoechst 33342, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.</p> <p><br /><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/hoechst-33342-emission-spectrum.gif" alt="Hoechst 33342 emission spectrum" /></p> <p class="caption"><strong>Fig. 2. Hoechst 33342 excitation and emission spectra.</strong><br />&nbsp;</p> <p>PureBlu Hoechst 33342 Nuclear Staining Dye is compatible with fixed and unfixed cells. Compared to DAPI, it exhibits greater permeability toward intact cell membranes and is preferred when conducting experiments with unfixed samples.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/zoe-fluorescent-cell-imager">ZOE&trade; Fluorescent Cell Imaging System</a></li> <li><a href="/en-us/product/tc20-automated-cell-counter?ID=M7FBG34VY">TC20&trade; Automated Cell Counter</a></li> </ul> <h2 class="descHeader">More Information</h2> <p><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10043283.pdf" target="_blank" rel="noopener noreferrer">PureBlu&trade; DAPI Nuclear Staining Dye Product Insert</a></p> /webroot/web/html/lsr/products/SKUs 135-1304.html /webroot/web/html/lsr/products/SKUs/135-1304.html Molecular formula 2963 C<sub>27</sub>H<sub>28</sub>N<sub>6</sub>O·3HCl 1 0 Molecular weight 2964 561.93 1 0 Excitation/emission wavelengths 2897 350/461 nm 1 0 CAS Number 2961 23491-52-3 1 0 Purity 2036 >95% (HPLC purified) 1 0 Solubility 2962 Soluble in water and dimethyl sulfoxide (DMSO) 1 0 Storage and use 2877 –20ºC 1 0 Temperature stability 2251 Stable for 2 years at –20°C <br />After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C 1 0 1351303 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1303_1403214125.xml 1450031 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/ZOE-Fluorescent-Cell-Imager/145-0031_1403030204.xml 1450102 /templatedata/internet/product_SKU/data/LSR/Cell-Counting/TC20-Automated-Cell-Counter/145-0102_1345072589.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&CartQuote=QT&quantity= 1108 false true false $113.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&AddToDefaultWishList=true&quantity= true false true 12171500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&quantity= 1351304
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Number Description Options
10043282 PureBlu™ DAPI Nuclear Staining Dye Product Insert, Rev A Click to download
10043283 PureBlu™ Hoechst 33342 Nuclear Staining Dye Product Insert, Rev A Click to download
6607 PureBlu™ Nuclear Staining Dyes Flier, Ver B Click to download [ Add to Cart (Free) ]

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