NGC™ 100 Medium-Pressure Chromatography Systems

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NGC 100 Medium-Pressure Chromatography Systems

Overview

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The NGC chromatography system introduces a new level of modularity and usability for protein purification.

Use our interactive NGC configurator to design your ideal chromatography system.

Fluidic scheme

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NGC™ Testimonials
NGC™ 100 System Comparison Chart
  NGC Chromatography Systems
Product Description NGC Quest™ 100
(788-0002)
NGC™ Quest 100 Plus
(788-0004)
NGC Scout™ 100
(788-0006)
NGC™ Scout 100 Plus
(788-0008)
NGC Discover™ 100
(788-0010)
NGC™ Discover 100 Pro
(788-0012)
NGC F100 Pump Module
NGC Mixer Module
NGC Sample Inject Valve Module
NGC
Single-Wavelength Detector Module
NGC Multi-Wavelength Detector Module
NGC Buffer Blending Valve Module
NGC pH Valve Module
NGC Sample Pump Module
NGC Inlet Valve Module
NGC Column Switching Valve Module, 100 ml
NGC Outlet Valve Module
NGC™ Systems and Modules — Specifications
System Specifications

Control system ChromLab™ Software
(compatible across all NGC Systems)
Dimensions (D x W x H) 61 x 49 x 56 cm
(NGC Quest™ and NGC Scout™ Systems)
61 x 49 x 74 cm
(NGC Discover™ System)
Weight
(excluding computer)
41–43 kg
(NGC Quest and NGC Scout Systems)
64 kg
(NGC Discover System)
Power supply 100–240 V, 50–60 Hz
Power consumption 750 W maximum

System Pump

Pump type Reciprocating piston
Flow rate setting 10 ml/min pumps: 0.001–10 ml/min (normal range)
100 ml/min pumps: 0.01–100 ml/min (normal range)
Flow rate accuracy ±2% (conditions: 0.1–10 ml/min, pressure
<600 psi (4.1 MPa, 41 bar), viscosity:
0.5–3.7 cP
Pressure range 10 ml/min pumps: 0–25.2 MPa (3,650 psi)
100 ml/min pumps: 0–10 MPa (1,450 psi)
Viscosity range 0.5–10.8 cP (for 10 ml/min and 100 ml/min pumps)

Sample Pump

Pump type Piston pump, metering type
Flow rate setting 0.01–100 ml/min
Flow rate accuracy ±2%
Pressure range 0–10 MPa (1,450 psi)
Viscosity range 0.5–10 cP

Mixer

Mixing principle Chamber with magnetic stirrer
Mixer volume 263 µl (included), 750 µl (included), 2 ml, 5 ml (for F10 pumps)
750 µl (included), 2 ml (included), 5 ml, 12 ml (for F100 pumps)
Gradient composition accuracy ±0.5% (conditions: 3–97% B, 0.25–10 ml/min for F10 pumps)
±0.5% (conditions: 3–97% B, 0.25–100 ml/min for F100 pumps)

Valves

Type Rotary valves and rocker solenoid
Number of valves Up to 12
Functions Loop selection
(PEEK Loop and DynaLoop™ Sample Loop offerings)

Pressure Sensors

Placement of sensors Standard: after system pump
Options: pre-column, post-column
Range 0–3,650 psi
Accuracy ±2 psi or 2%, whichever is greater

Inlet Valves

Inlet A 8 inlets
Inlet B 8 inlets
Sample inlet* Up to 16 inlets


* Coming soon.

UV and UV/Vis Detectors

(each includes an integrated conductivity monitor)
Wavelength Single-wavelength: 255 nm (nucleic acids)
or 280 nm (proteins)
Multi-wavelength (up to 4): 190–800 nm
Absorbance range 0 to >2.8 Au
Linearity 0–2 Au within ±5%
Operating pressure 1,450 psi (10 MPa) for 5, 10 mm flow cells
700 psi (5 MPa) for 2 mm flow cells
Flow cells
Operational interchangeable flow cells available in path lengths of 2, 5, and 10 mm
Preparative: 2 mm (cell volume: 20 µl)
Analytical: 5 mm (cell volume: 9 µl)
Analytical: 10 mm (cell volume: 18 µl)

Conductivity Monitor

Conductivity reading range 0.01 µS/cm–999 mS/cm
Accuracy ±2%
Operating pressure 0–5.5 MPa (800 psi)
Flow cell volume 6 µl
Temperature monitor range 4–100°C
Temperature monitor accuracy ±2%

pH Monitor

pH reading range 0–14
Accuracy ±0.1 pH unit within pH 2–12
Operating pressure 0–70 psi with pH probe inline
and 0–500 psi in bypass mode
Flow cell volume 100 µl (210 µl including internal flow paths)

Column Switching

Five-column valve Can connect up to 5 columns with forward and reverse flow and bypass capability

Buffer Blending

 Buffer blending valve
Standard in the NGC Scout and NGC Discover Systems

Air Sensor

Number of sensors Up to 8 total air sensors (1 for end-of-sample detection, remaining are for end-of-buffer detection)
Accuracy ±0.1 pH unit within pH 2–12
Placement of built-in sensors End of buffer, end of sample
Sensing principle Acoustic


Note:
All NGC Systems include a touch screen and are compatible with the BioFrac™ Fraction Collector and C-96 Autosampler.



Try an NGC System
in your lab
Request a Demo

The family of NGC Medium-Pressure Chromatography Systems fit your throughput requirements when purifying biomolecules for analytical- and preparative-scale applications. Each system is composed of interchangeable modules that confer different system capabilities.

The six preconfigured NGC Liquid Chromatography Systems listed below are designed with increasing automation and throughput to serve a wide range of laboratory needs:

  • NGC Quest™ 100 (7880002) and NGC™ Quest 100 Plus Systems (7880004) — for separating biomolecules at high resolution using accurate gradients
  • NGC Scout™ 100 (7880006) and NGC™ Scout 100 Plus Systems (7880008) — for medium throughput applications requiring rapid scouting of protein purification conditions with automated gradients and buffer preparation
  • NGC Discover™ 100 (7880010) and NGC™ Discover 100 Pro Systems (7880012) — for higher throughput applications with rapid, robust automation and a need for method development

For an overview of the features and capabilities of the six NGC Chromatography Systems, view the NGC 100 System comparison chart and watch the NGC System Tour.

The NGC Systems are composed of interchangeable modules that confer different system capabilities. Browse the module selection guide to help you decide which system is best for you, or use our online tool to configure your personalized NGC Instrument.

Features and Benefits of the NGC 100 ml Chromatography Systems

Flexible, customizable chromatography systems to suit both your application and workflow needs:

  • The guided fluidics selection tool (patent pending), coupled with the module discovery system, enables a single-click application-based system setup through automated module function recognition
  • The Tier Rotate™ Feature allows optimal placement of valves and detectors to minimize the flow path
  • Compact footprint — fits in a refrigerator, lab bench, or coldroom
  • Point-to-Plumb™ Feature shows the step-by-step plumbing of the system using LEDs
  • ChromLab™ Software provides powerful system control, automated methods, and analysis options

Applications and Uses of Medium-Pressure Chromatography Systems

Related Products

Bio-Rad also offers NGC™ Chromatography Systems with integrated 10 ml system pumps as well as a variety of chromatography columns and resins.

Compatible Chromatography Columns

Designed specifically for NGC Chromatography Systems, these prepacked high-resolution columns work at high pressures and are optimized for the separation of biomolecules at fast flow rates.

ENrich™ Ion Exchange Columns ENrich™ Size Exclusion Columns
 

More Information

Upgrade your NGC Chromatography System by adding more modular components to match your changing throughput and application requirements:

See how the NGC™ System benefits University of California researchers by increasing throughput and making it easier for students to become experts at protein purification.

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NGC Quest™ 100 Chromatography System

7880002
Includes 100 ml/min pumps, single-wavelength (UV) and conductivity detection, and automated sample injection, for the purification of proteins

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NGC Quest™ 100 Plus Chromatography System

7880004
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, and automated sample injection, for the purification of proteins

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NGC Scout™ 100 Chromatography System

7880006
Includes 100 ml/min pumps, single-wavelength (UV) and conductivity detection, buffer blending options, for rapid pH scouting during sample purification

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NGC Scout™ 100 Plus Chromatography System

7880008
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, buffer blending options, and a pH valve, for rapid pH scouting during the purification of proteins, peptides, and nucleic acids

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NGC Discover™ 100 Chromatography System

7880010
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve, and column switching of up to 5 columns, for method development

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NGC Discover 100 Pro Chromatography System
NGC Discover™ 100 Pro Chromatography System

7880012
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve and column switching of up to 5 columns, for method development

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Number Description Options
6494 Protein Purification Workflow with the NGC™ Chromatography System and Stain-Free Gels Brochure, Rev C Click to download [ Add to Cart (Free) ]
6286 NGC™ Chromatography Systems Brochure, Rev E Click to download [ Add to Cart (Free) ]
6326 NGC™ Chromatography Systems Module Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
10000049090 Instruction Manual, NGC™ Chromatography Systems and ChromLab™ Software 4.0 Installation Guide, Ver A Click to download
10000049091 Instruction Manual, NGC™ Chromatography Systems and ChromLab™ Software 4.0 Instrument Guide, Ver A Click to download
10000049092 Instruction Manual, NGC™ Chromatography Systems and ChromLab™ Software 4.0 User Guide, Ver A Click to download
6608 NGC™ Chromatography Systems for Protein Purification in Drug Discovery and Development Brochure, Ver C Click to download [ Add to Cart (Free) ]
6674 NGC™ Chromatography Systems Multidimensional (Multi-D) Plumbing Guide, Ver B Click to download
6377 Chromatography Success Guide Click to download
6701 Multidimensional (Multi-D) Chromatography Success Guide, Ver B Click to download
6694 Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography, Ver B Click to download
6805 Proven Method for Confirming Protein Purity and Yield Brochure, Ver B Click to download [ Add to Cart (Free) ]
6843 NGC™ Chromatography System Applications Publication List, Ver A Click to download