NGC™ 10 ml Medium-Pressure Chromatography Systems

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NGC 10 ml Chromatography Systems

Overview

NGC™ Configurator



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The NGC chromatography system introduces a new level of modularity and usability for protein purification.

Use our interactive NGC configurator to design your ideal chromatography system.

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NGC™ Testimonials
NGC™ 10 System Comparison Chart
  NGC Chromatography Systems
Product Description NGC Quest™ 10
(788-0001)
NGC™ Quest 10 Plus
(788-0003)
NGC Scout™ 10
(788-0005)
NGC™ Scout 10 Plus
(788-0007)
NGC Discover™ 10
(788-0009)
NGC™ Discover 10 Pro
(788-0011)
NGC F10 Pump Module
NGC Mixer Module
NGC Sample Inject Valve Module
NGC Single-Wavelength Detector Module
NGC Multi-Wavelength Detector Module
NGC Buffer Blending Valve Module
NGC pH Valve Module
NGC Sample Pump Module
NGC Inlet Valve Module
NGC Column Switching Valve Module, 10 ml
NGC Outlet Valve Module
NGC™ Systems and Modules — Specifications
System Specifications

Control system ChromLab™ software 2.0
(compatible across all NGC systems)
Dimensions (D x W x H) 61 x 49 x 56 cm
(NGC Quest™ and NGC Scout™ systems)
61 x 49 x 74 cm
(NGC Discover™ system)
Weight
(excluding computer)
41ndash;43 kg
(NGC™ Quest and NGC Scout™ system)
64 kg
(NGC Discover™ system)
Power supply 100–240 V, 50–60 Hz
Power consumption 750 W maximum

System Pump

Pump type Reciprocating piston
Flow rate setting 10 ml/min pumps: 0.001–10 ml/min (normal range)
100 ml/min pumps: 0.01–100 ml/min (normal range)
Flow rate accuracy ±2% (conditions: 0.1–10 ml/min, pressure
<600 psi (4.1 MPa, 41 bar), viscosity:
0.5–3.7 cP
Pressure range 10 ml/min pumps: 0–25.2 MPa (3,650 psi)
100 ml/min pumps: 0–10 MPa (1,450 psi)
Viscosity range 0.5–10.8 cP (for 10 ml/min and 100 ml/min pumps)

Sample Pump

Pump type Piston pump, metering type
Flow rate setting 0.01–100 ml/min
Flow rate accuracy ±2%
Pressure range 0–10 MPa (1,450 psi)
Viscosity range 0.5–10 cP

Mixer

Mixing principle Chamber with magnetic stirrer
Mixer volume 263 µl (included), 750 µl (included), 2 ml, 5 ml (for F10 pumps)
750 µl (included), 2 ml (included), 5 ml, 12 ml (for F100 pumps)
Gradient composition accuracy ±0.5% (conditions: 3–97% B, 0.25–10 ml/min for F10 pumps)
±0.5% (conditions: 3–97% B, 0.25–100 ml/min for F100 pumps)

Valves

Type Rotary valves and rocker solenoid
Number of valves Up to 12
Functions Loop selection
(PEEK loop and DynaLoop™ sample loop offerings)

Pressure Sensors

Placement of sensors Standard: after system pump
Options: pre-column, post-column
Range 0–3,650 psi
Accuracy ±2 psi or 2%, whichever is greater

Inlet Valves

Inlet A 8 inlets
Inlet B 8 inlets
Sample inlet* Up to 16 inlets


* Coming soon.

UV and UV/Vis Detectors

(each includes an integrated conductivity monitor)
Wavelength Single-wavelength: 255 nm (nucleic acids)
or 280 nm (proteins)
Multi-wavelength (up to 4): 190–800 nm
Absorbance range 0 to >2.8 Au
Linearity 0–2 Au within ±5%
Operating pressure 1,450 psi (10 MPa) for 5, 10 mm flow cells
700 psi (5 MPa) for 2 mm flow cells
Flow cells
Operational interchangeable flow cells available in path lengths of 2, 5, and 10 mm
Preparative: 2 mm (cell volume: 20 µl)
Analytical: 5 mm (cell volume: 9 µl)
Analytical: 10 mm (cell volume: 18 µl)

Conductivity Monitor

Conductivity reading range 0.01 µS/cm–999 mS/cm
Accuracy ±2%
Operating pressure 0–5.5 MPa (800 psi)
Flow cell volume 6 µl
Temperature monitor range 4–100°C
Temperature monitor accuracy ±2%

pH Monitor

pH reading range 0–14
Accuracy ±0.1 pH unit within pH 2–12
Operating pressure 0–70 psi with pH probe inline
and 0–500 psi in bypass mode
Flow cell volume 100 µl (210 µl including internal flow paths)

Column Switching

Five-column valve Can connect up to 5 columns with forward and reverse flow and bypass capability

Buffer Blending

  Standard in the NGC Scout and NGC Discover systems

Air Sensor

Number of sensors Up to 8 total air sensors (1 for end of sample detection, remaining are for end of buffer detection)
Accuracy ±0.1 pH unit within pH 2–12
Placement of built-in sensors End of buffer, end of sample
Sensing principle Acoustic


Note:
All NGC systems include a touch screen and are compatible with the BioFrac™ fraction collector and C-96™ autosampler.

The family of NGC Medium-Pressure Chromatography Systems offers a single laboratory chromatography solution that scales to fit your throughput requirements when purifying biomolecules for analytical- and preparative-scale applications. Each system is composed of interchangeable modules that confer different system capabilities.

For an overview of the features and capabilities of the NGC Systems, watch the NGC™ System Tour and download the NGC™ System brochure.

Six preconfigured instruments — the NGC Quest™ 10, NGC™ Quest 10 Plus, NGC Scout™ 10, NGC™ Scout 10 Plus, NGC Discover™ 10, and NGC™ Discover 10 Pro Liquid Chromatography Systems — are designed with increasing automation and throughput to serve a wide range of laboratory needs. For a complete list of modules within each system, view the NGC 10 system comparison chart. Use the module selection guide to help you decide which system is best for you.

If you prefer to customize your NGC System, use our online tool to configure your personalized NGC™ Instrument.

NGC Quest 10 and NGC Quest 10 Plus Systems

The NGC Quest™ 10 and NGC™ Quest 10 Plus Chromatography Systems are designed for the separation of biomolecules at high resolution with the use of accurate gradients. Both systems offer:

  • Automated 10 ml/min pumps that generate accurate gradients for high-resolution separations for any application
  • Automated sample injection using fixed or dynamic loops
  • Gradient separations at different pH values using the mixer module, for rapid method development
  • Compatibility with the C-96™ Autosampler, available with or without cooling, for accurate and reproducible sample injections
  • Compatibility with the BioFrac™ Fraction Collector for sample collection
  • Single-wavelength detection with the NGC Quest 10 System — ideal for high-accuracy protein detection with simultaneous conductivity measurements
  • Multi-wavelength detection with the NGC Quest 10 Plus System — detection of up to four wavelengths — ideal for measuring conductivity while simultaneously detecting proteins, peptides, nucleic acids, and chromogenic molecules

NGC Scout 10 and NGC Scout 10 Plus Systems

The NGC Scout™ 10 and NGC™ Scout 10 Plus Chromatography Systems are designed for medium throughput applications that require rapid scouting of protein purification conditions with automated gradients and buffer preparation.

Both NGC Scout Systems include the capabilities of the corresponding lower throughput systems, the NGC Quest 10 and NGC Quest 10 Plus Instruments, but also comprise:

  • A buffer blending valve — creates automated buffer gradients during automated inline buffer preparation, for rapid scouting of purification conditions
  • A pH valve — allows monitoring buffer pH and gradient separations at different pH values, for rapid method development
  • A single-wavelength detector (NGC Scout 10 System)
  • A multi-wavelength detector (NGC Scout 10 Plus System)

NGC Discover 10 and NGC Discover 10 Pro Systems

The NGC Discover™ 10 and NGC™ Discover 10 Pro Chromatography Systems are designed for higher throughput applications with rapid, robust automation and a need for method development.

Both NGC Discover 10 Systems include the capabilities of the NGC Scout 10 Plus Instruments but also comprise:

  • A 100 ml/min integrated sample pump — supports contamination-free automated sample application
  • Sample inlet valves — enable automated switching between buffers and columns, accelerating method development, column cleaning, and regeneration
  • A 10 ml column switching valve — facilitates automated column/media scouting without the need to replumb
  • A sample outlet valve — only with the NGC Discover 10 Pro System — allows the collection of up to 12 large-volume fractions

Features and Benefits of the NGC 10 ml Chromatography Systems

Flexible, customizable chromatography systems to suit both your application and workflow needs:

  • The guided fluidics selection tool (patent pending), coupled with the module discovery system, enables a single-click application-based system setup through automated module function recognition
  • The Tier Rotate™ Feature allows optimal placement of valves and detectors to minimize the flow path
  • Compact footprint — fits in a refrigerator, lab bench, or coldroom
  • Point-to-Plumb™ Feature shows the step-by-step plumbing of the system using LEDs
  • ChromLab™ Software provides powerful system control, automated methods, and analysis options

Applications and Uses of Medium-Pressure Chromatography Systems

More Information

Upgrade your NGC Chromatography System by adding more modular components to match your changing throughput and applications requirements:

If you prefer to customize your NGC System using our online tool, configure your personalized NGC™ Instrument.

See how the NGC™ System benefits University of California researchers by increasing throughput and making it easier for students to become experts at protein purification.

NGC Quest 10 Chromatography System #788-0001 - NGC 10 ml Medium-Pressure Chromatography Systems
NGC Quest™ 10 Chromatography System

788-0001
Includes 10 ml/min pumps, single-wavelength (UV) and conductivity detection, and automated sample injection, for the purification of proteins

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NGC Quest 10 Plus Chromatography System #788-0003 - NGC 10 ml Medium-Pressure Chromatography Systems
NGC Quest™ 10 Plus Chromatography System

788-0003
Includes 10 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, and automated sample injection, for the purification of proteins

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NGC Scout 10 Chromatography System #788-0005 - NGC 10 ml Medium-Pressure Chromatography Systems
NGC Scout™ 10 Chromatography System

788-0005
Includes 10 ml/min pumps, single-wavelength (UV) and conductivity detection, buffer blending options, for rapid pH scouting during sample purification

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NGC Scout 10 Plus Chromatography System #788-0009 - NGC 10 ml Medium-Pressure Chromatography Systems
NGC Scout™ 10 Plus Chromatography System

788-0007
Includes 10 ml/min pumps, multi-wavelength (UV/Vis) detection, buffer blending options, for rapid pH scouting during the purification of proteins, peptides, and nucleic acids

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NGC Discover 10 Chromatography System #788-0009 - NGC 10 ml Medium-Pressure Chromatography Systems
NGC Discover™ 10 Chromatography System

788-0009
Includes 10 ml/min pumps, multi-wavelength (UV/Vis) detection, expanded scouting options, large-sample injection, and column switching of up to 5 columns, for method development

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NGC Discover™ 10 Pro Chromatography System

788-0011
Includes 10 ml/min pumps, multi-wavelength (UV/Vis) detection, expanded scouting options, large-sample injection, outlet valve, and column switching of up to 5 columns, for method development

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Number Description Options
6529 Protein Purification and Crystallization of Diabetes Related Peroxisome Proliferator–Activated Receptor-γ (PPARγ), Rev A Click to download [ Add to Cart (Free) ]
6297 Foresight™ Prepacked Plates and Columns Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6286 NGC™ Chromatography Systems Brochure, Rev C Click to download [ Add to Cart (Free) ]
6326 NGC Chromatography Systems Module Selection Guide, Rev A Click to download [ Add to Cart (Free) ]
10026253 Instruction Manual, NGC Chromatography Systems and ChromLab Software 3.0 Instrument Guide, Rev C Click to download
10026255 Instruction Manual, NGC Chromatography Systems and ChromLab Software 3.0 User Guide, Rev C Click to download
6530 Protein Purification Workflow Development Using Bio-Rad’s NGC Chromatography System, Rev B Click to download