Mouse Diabetes Assays

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Overview

Bio-Plex Pro™ Mouse Diabetes Assays
Assay Menu* 8-Plex Panel (171-F7001M) Adiponectin All-in-One Format (171-F7002M)
Bead Regions
 
Adiponectin **
29
Ghrelin
64
GIP
46
GLP-1
62
Glucagon
63
Insulin
66
Leptin
65
PAI-1
48
Resistin
30


* With the exception of adiponectin, all assays may be ordered as either customized Express assays or as singleplex sets (vial of beads coupled to capture antibodies and vial of detection antibodies). Requires standard and Bio-Plex Pro reagent kit to perform an assay. To learn more about Express assays, go to www.bio-rad.com/bio-plex/assaybuilder.

** Due to different dilution scheme, adiponectin is offered in an all-in-one format only.

Calculation Worksheet for Bio-Plex Pro Assays
Bio-Plex Pro Mouse Diabetes Assay Validation

Mouse Diabetes Assay chart

Levels of biomarkers of normal () and diabetic () groups. Student’s t-test was used to determine statistical significance between groups. Black lines denote mean values. P values are indicated for diabetes markers that were significantly different from normal samples (P<0.05).

Mouse Diabetes Linearity of Dilution
Linearity of dilution

Linearity of dilution. Linearity of analyte measurements in either serum or plasma (3-fold) were measured using linear regression analysis. Result is shown for the GLP-1 assay diluted in serum. R2 value was 0.99 or higher within the assay working range for all 9 assays in both serum and plasma.

Specifications
Incubation Time 1 Hour
Sample Volume 12.5 µl
Accuracy (Recovery Range) 80-120%
Precision
  Intra-Assay %CV
  Inter-Assay %CV
 
≤10%
≤15%
Cross-reactivity Negligible

Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are the mean data determined from three independent multiplex assays in a serum-based matrix. LLOQ and ULOQ are defined as the boundary standard curve points for which the performance specifications were met for 10% intra-assay CV, 15% inter-assay CV, and recovery range of 80–120%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

Targets
LLOQ
ULOQ
LOD
Intra-assay %CV
Inter-assay %CV
Adiponectin*
38.0
 
62,043
 
8.4
 
4
3
8-Plex Assays            
Ghrelin
3.1
 
7,296
 
0.8
 
5
4
GIP
13.4
 
14,999
 
2.3
 
4
10
GLP-1
3.4
 
1,969
 
0.8
 
6
11
Glucagon
24.0
 
3,067
 
7.0
 
6
6
Insulin
93.4
 
47,815
 
22.0
 
6
4
Leptin
17.1
 
69,900
 
6.2
 
4
3
PAI-1
0.7
 
2,922
 
0.5
 
5
2
Resistin
125.9
 
257,870
 
32.0
 
4
4

*Due to the different dilution scheme, adiponectin was assayed as a single assay.

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ). Precision is expressed as the coefficient of variation (%CV) at concentrations within the assay working range. Accuracy is expressed as a ratio of the observed concentration vs. the expected concentration of a known amount of spiked analyte within the assay working range.

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal.

Bio-Plex® Technology and Applications

The Bio-Plex Pro™ mouse diabetes immunoassays offer reliable performance and a convenient format for studying mouse diabetes and obesity markers.

These assays are magnetic bead–based assays, available as either premixed panels or singleplex sets, that rapidly (3 hr) detect multiple diabetes and obesity biomarkers in a single experiment, using as little as 12.5 µl of sample. The 8-plex assay detects:

  • Ghrelin
  • GIP
  • GLP-1
  • Glucagon
  • Insulin
  • Leptin
  • PAI-1 (total)
  • Resistin

Additionally, adiponectin can also be assayed; it is available as a singleplex analyte. Due to different dilution schemes, adiponectin should be assayed separately from the eight markers in the panel. Based on 6.5 μm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic beads for simplified plate processing
  • Results obtained in 3 hr due to short 1 hr incubation time
  • All-in-one, space-saving complete kit
  • Premixed standards for generation of curves over a broad dynamic range for each target

Other Assay Formats Available

  • Custom assays (mixed at the bench): available as Bio-Plex® Express assays
  • Singleplex sets: each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (#171-304070) and a vial of standards (#171-I70001) for a complete assay

For multiplexing capabilities with cytokine assays, refer to bulletin 5975.

Instrument Compatibility

  • Bio-Plex 200 system
  • Bio-Plex 3D system
  • Bio-Plex Pro wash station
  • Bio-Plex Pro II wash station

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Immunoassay for Diabetes
Species Mouse
Bead type Magnetic
Custom assay options Express
Incubation time, hr 1
Sample volume, µl 12.5
Accuracy (recovery range) 80–120%
Cross-reactivity Negligible
Bio-Plex Pro Mouse Diabetes 8-Plex Assay #171-F7001M
Bio-Plex Pro Mouse Diabetes 8-Plex Assay

171-F7001M
1 x 96-well, includes conjugated magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, flat bottom plate, sealing tape, for the detection of 8 mouse diabetes biomarkers

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Bio-Plex Pro Mouse Diabetes Adiponectin Assay #171-F7002M
Bio-Plex Pro Mouse Diabetes Adiponectin Assay

171-F7002M
1 x 96-well, includes conjugated magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, flat bottom plate, sealing tape, for the detection of mouse adiponectin

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Bio-Plex Pro Mouse Diabetes 8-plex Panel, 10x96-well #171-F7010M
Bio-Plex Pro Mouse Diabetes 8-plex Panel, 10x96-well

171-F7010M
Bio-Plex Pro Mouse Diabetes 8-plex Panel, 10x96-well, includes premixed coupled magnetic beads and detection antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, filter plate, sealing tape, standard diluent, sample diluent for the detection of Ghrelin, GIP, GLP-1, Glucagon, Insulin, Leptin, PAI-1, Resistin

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Bio-Plex Pro Mouse Diabetes Ghrelin Set

171-G7002M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse ghrelin, requires diabetes reagent kit and standards

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Bio-Plex Pro Mouse Diabetes GIP Set

171-G7003M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse GIP, requires diabetes reagent kit and standards

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Bio-Plex Pro Mouse Diabetes GLP-1 Set

171-G7004M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse GLP-1, requires diabetes reagent kit and standards

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Bio-Plex Pro Mouse Diabetes Glucagon Set

171-G7005M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse glucagon, requires diabetes reagent kit and standards

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Bio-Plex Pro Mouse Diabetes Insulin Set

171-G7006M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse insulin, requires diabetes reagent kit and standards

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Bio-Plex Pro Mouse Diabetes Leptin Set

171-G7007M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse leptin, requires diabetes reagent kit and standards

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Bio-Plex Pro Mouse Diabetes PAI-1 Set

171-G7008M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse PAI-1, requires diabetes reagent kit and standards

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Bio-Plex Pro Mouse Diabetes Resistin Set

171-G7009M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for the detection of mouse resistin, requires diabetes reagent kit and standards

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Number Description Options
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download [ Add to Cart (Free) ]
5975 Multiplexing Compatibility of the Bio-Plex Pro Diabetes and Cytokine Assays, Rev A Click to download
5986 Mouse Diabetes Multiplex Metabolic Biomarkers Assay, Rev A Click to download [ Add to Cart (Free) ]
6000 Development of a Magnetic Bead-based Multiplex Immunoassay for Metabolic Biomarkers in Normal and Diabetic Mouse Serum and Plasma Samples, Rev A Click to download
10010747 Instruction Manual, Bio-Plex Pro Diabetes Assays, Rev D Click to download
10010747_45-49 Calculation Worksheet for Bio-Plex Pro Assays Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
6342 Bio-Plex Pro Diabetes Assay Panels Product Information Sheet, Rev A Click to download
10024973 Bio-Plex Pro Assays Quick Guide 2, Rev A Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download