Molecular Mass Rulers

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Overview

Specifications
Contents 1 vial containing 250 µl of the following mixture:
Stock solution When used at 2.5 µl per lane
10 µg / 1,000 bp 100 ng / 1,000 bp
7 µg / 700 bp 70 ng / 700 bp
5 µg / 500 bp 50 ng / 500 bp
2 µg / 200 bp 20 ng / 200 bp
1 µg / 100 bp 10 ng / 100 bp
Quantity DNA sufficient for 100 lanes at 2.5 µl per lane.
Concentration 100 µg/ml in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8)

The DNA fragments in this product possess either EcoRI or Hind III- compatible cohesive ends.

Contents 1 vial EZ Load precision molecular mass ruler, 500 µl supplied in 5% glycerol, 15 nM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF
Quantity DNA sufficient for 100 lanes at 5 µl per lane
100 ng / 1,000 bp
70 ng / 700 bp
50 ng / 500 bp
20 ng / 200 bp
10 ng / 100 bp
Concentration 50 µg/ml
Molecular Mass Ruler Selection Guide
Molecular Mass Ruler

Precision Molecular Mass Ruler/EZ Load Precision Molecular Mass Ruler

Comparison of Molecular Mass Rulers
  Concentration Range Number of Bands Amount Suggested Gel Type Number of Applications
Precision 0.1 µg/µl 100–1,000 bp 5, from 10 to 100 ng 25 µg DNA 1–3% agarose 100
EZ Load™ Precision 0.05 µg/µl 100–1,000 bp 5, from 10 to 100 ng 25 µg DNA 1–3% agarose 100

Bio-Rad precision molecular mass rulers (DNA ladders) allow accurate DNA quantitation in gels, making them ideal for densitometry or image analysis. These standards have five bands of decreasing DNA mass: 100 ng in a 1,000 bp band, 70 ng in a 700 bp band, 50 ng in a 500 bp band, 20 ng in a 200 bp band, and 10 ng in a 100 bp band. The EZ Load precision molecular mass ruler has been blended with sample loading buffer and is ready to load.

Precision Molecular Mass Ruler

170-8207
250 μl, 0.1 μg/μl, DNA standard for quantitation in gels, 100–1,000 bp, 5 bands at 10 to 100 ng, 100 applications

List Price:   $112.00
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EZ Load™ Precision Molecular Mass Ruler

170-8356
500 μl, 0.05 μg/μl, ready-to-load DNA standard for quantitation in gels, 100–1,000 bp, 5 bands at 10 to 100 ng, 100 applications

List Price:   $112.00
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Number Description Options
4100094 Instruction Manual, Precision Molecular Mass Rulers Click to download
4006137 Instruction Manual, EZ Load Molecular Rulers Click to download
2121 User Guide to Nucleic Acid Standards Bulletin, Rev D Click to download
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]