Model 785 Vacuum Blotter

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Overview

Northern Transfer

Left to right, 1.0, 2.5, 5, 10, or 20 µg of total RNA from CHO HA-1 cells was separated on a glyoxal gel and transferred to a Zeta-Probe membrane using a vacuum of 3" Hg for 90 min. The blot was probed with a 32P-labeled hsp70 cDNA fragment and exposed to X-ray film overnight. Probe and RNA provided by Gloria Lee, University of California, San Francisco, CA USA.

Southern Transfer

Human genomic DNA (5 µg) was cleaved with (left to right) BglI, HindIII, or NheI, separated on a 1% Certified molecular biology agarose gel, and transferred to a Zeta-Probe GT membrane using a vacuum of 5" Hg for 90 min. The blot was probed with a 32P-labeled factor VIII fragment and exposed to X-ray film overnight. Probe provided by the Genetics Institute of Boston.

Discontinued Product
The Model 785 Vacuum Blotter has been discontinued. However, we will continue to provide support for customers with Model 785 units.

 

The Model 785 Vacuum Blotter quickly and efficiently transfers DNA or RNA from an agarose gel onto a nylon membrane. The blotter produces even transfers from top to bottom and side to side, regardless of the gel size. The unique regulator makes it simple to control pressure from an in-house vacuum or a Bio-Rad vacuum pump (included with the complete system), avoiding high pressures that can lead to gel collapse and DNA trapping.

Assembly of the unit is easy. The vacuum blotter has a simple snap-on sealing frame. Gel-to-membrane alignment is easy using the green window gaskets, which can be cut to accommodate gels as large as 20 x 25 cm. The sealing frame requires no screws or clamps to tighten; just press into place to seal in the vacuum. Because small fragments (≤1,500 bp) transfer in 30 minutes and genomic DNA-size fragments transfer in 90 minutes, the hybridization reaction can be started on the same day.

The vacuum blotter is made of a durable molded plastic that is resistant to the chemicals commonly used in transfers, such as 0.25 N HCl and 0.5 N NaOH. The window gaskets are also resistant to these chemicals.

(Discontinued) Model 785 Vacuum Blotter With Regulator

165-5000
Vacuum transfer apparatus, includes vacuum regulator, porous vacuum plate, reservoir seal O-ring, sealing frame, assorted gaskets, lid

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Model 785 Vacuum Blotter System

165-5001
100/120 V, vacuum transfer system, includes vacuum pump, vacuum regulator, porous vacuum plate, reservoir seal O-ring, sealing frame, assorted gaskets, lid

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Model 785 Vacuum Blotter Basic Unit

165-5003
Vacuum transfer apparatus, includes porous vacuum plate, reservoir seal O-ring, sealing frame, assorted gaskets, lid

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
1584 Model 785 Vacuum Blotter Product Information Sheet, Rev B Click to download
M1655000 Instruction Manual, Model 785 Vacuum Blotter, Rev F Click to download