Model 785 Vacuum Blotter

Model 785 Vacuum Blotter

Overview

The Model 785 vacuum blotter quickly and efficiently transfers DNA or RNA from an agarose gel onto a nylon membrane. The unique regulator makes it simple to control pressure.
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Description

Description

Discontinued Product

The Model 785 Vacuum Blotter has been discontinued. However, we will continue to provide support for customers with Model 785 units.

The Model 785 Vacuum Blotter quickly and efficiently transfers DNA or RNA from an agarose gel onto a nylon membrane. The blotter produces even transfers from top to bottom and side to side, regardless of the gel size. The unique regulator makes it simple to control pressure from an in-house vacuum or a Bio-Rad vacuum pump (included with the complete system), avoiding high pressures that can lead to gel collapse and DNA trapping.

Assembly of the unit is easy. The vacuum blotter has a simple snap-on sealing frame. Gel-to-membrane alignment is easy using the green window gaskets, which can be cut to accommodate gels as large as 20 x 25 cm. The sealing frame requires no screws or clamps to tighten; just press into place to seal in the vacuum. Because small fragments (≤1,500 bp) transfer in 30 minutes and genomic DNA-size fragments transfer in 90 minutes, the hybridization reaction can be started on the same day.

The vacuum blotter is made of a durable molded plastic that is resistant to the chemicals commonly used in transfers, such as 0.25 N HCl and 0.5 N NaOH. The window gaskets are also resistant to these chemicals.

Ordering

Ordering

items

Use the filters below to refine results!

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1655000
Vacuum transfer apparatus, includes vacuum regulator, porous vacuum plate, reservoir seal O-ring, sealing frame, assorted gaskets, lid
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1655001
100/120 V, vacuum transfer system, includes vacuum pump, vacuum regulator, porous vacuum plate, reservoir seal O-ring, sealing frame, assorted gaskets, lid
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1655003
Vacuum transfer apparatus, includes porous vacuum plate, reservoir seal O-ring, sealing frame, assorted gaskets, lid

Accessories

Accessories

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(Discontinued) Porous Vacuum Plate
1655012
Pkg of 1, for use with Model 785 vacuum transfer systems
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(Discontinued) Blank Window Gasket
1655008
Pkg of 6, for use with Model 785 vacuum transfer systems
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1655007
Pkg of 1, for use with Model 785 vacuum transfer systems
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(Discontinued) Vacuum Regulator
1655006
Pkg of 1, for use with Model 785 vacuum transfer systems
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(Discontinued) Vacuum Station
1655005
220/240 V, vacuum pump and regulator, for use with Model 785 vacuum blotter basic unit (#165-5003)
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(Discontinued) Vacuum Station
1655004
100/120 V, vacuum pump and regulator, for use with Model 785 vacuum blotter basic unit (#165-5003)

Kits & Reagents

Kits & Reagents

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(Discontinued) Gel Drying Solution
1610752
1 L, gel drying solution for use with polyacrylamide gels

Documents

Documents

Number Description Options
1584
Model 785 Vacuum Blotter Product Information Sheet, Rev B
M1655000
Instruction Manual, Model 785 Vacuum Blotter, Rev F