Model 491 Prep Cell and Mini Prep Cell

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Overview


Specifications
Specification Mini-Prep Cell Model 491 Prep Cell
Sample capacity (mass/volume) 0.5–1,000 µg/50–500 µl 1–500 mg/0.5–15 ml
Gel tube dimensions 7 mm ID, 13 cm length 28 and 37 mm ID, 14 cm length
Cooling Not necessary Glazed alumina-ceramic tube, peristaltic pump
Electrical limits 500 V, 10 mA, 5 W 500 V, 40 mA, 20 W
Elution buffer flow rate 0.1 ml/min 1 ml/min
Dimensions (W x D x H) 12 x 27 cm 19 x 35 cm
Weight 1.4 kg (3.1 lb) 2.7 kg (6.0 lb)

Fractionation by size (molecular weight) is an effective enrichment strategy for studies of protein families and posttranslational modifications, because the sizes of these proteins tend to be similar.

The Model 491 and mini prep cells separate biomolecules quickly and efficiently by continuous-elution electrophoresis. Samples are electrophoresed through a cylindrical gel matrix, where they are separated into ring-shaped bands. As individual bands migrate off the bottom of the gel, they are collected in discrete liquid fractions available for assay and characterization.

The Model 491 prep cell and the mini prep cell:

  • Allow resolution of proteins differing in molecular weight by as little as 2%
  • Separate up to 500 mg of total protein
  • Can be used as a complementary separation strategy to 2-D gels as well as for downstream protein purification

The Model 491 prep cell has a sample capacity of 1–500 mg/0.5–15 ml, while the mini prep cell has a sample capacity of 0.5–1,000 µg/50–500 µl.

Accessory equipment required to run the Model 491 and mini prep cells includes a fraction collector and power supply such as the PowerPac HV power supply. The Model 491 prep cell also requires a peristaltic pump.

Model 491 Prep Cell Without Buffer Recirculation Pump

170-2928
Continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume)

List Price:   $4,545.00
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Mini Prep Cell without Reagent Starter Kit

170-2908
Continuous-elution electrophoresis cell, 0.5-1.0 mg/50–500 µl sample capacity (mass/volume)

List Price:   $1,957.00
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Mini Prep Cell with Reagent Starter Kit

170-2915
Continuous-elution electrophoresis cell, 0.5-1.0 mg/50–500 µl sample capacity (mass/volume), includes reagent starter kit

List Price:   $2,137.00
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Model 491 Prep Cell

170-2926
100/120 V, continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume) includes pump, starter kit with protein standard

List Price:   $5,145.00
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Model 491 Prep Cell

170-2927
220/240 V, continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume) includes pump, starter kit with protein standard

List Price:   $4,996.00
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Number Description Options
3161 Model 491 Prep Cell Bio-Rad Literature Click to download
M1702925 Instruction Manual, Model 491 Prep Cell, Rev D Click to download
LIT466 Instruction Manual, Mini Prep Cell and Model 491 Prep Cell Starter Kit Click to download