Mini-PROTEAN® Tetra Handcast Systems

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Overview

Maximum Sample Volume per Well for Mini-PROTEAN® Tetra Cell Combs
  Comb Thickness
Number or Type of Wells Well Width 0.75 mm 1.0 mm 1.5 mm
5 12.70 mm 70 µl 105 µl 160 µl
9 5.08 mm 33 µl 44 µl 66 µl
10 5.08 mm 33 µl 44 µl 66 µl
15 3.35 mm 20 µl 26 µl 40 µl
IPG 6.20 mm 420 µl 730 µl
Prep/2-D
       
   Reference well 3.10 mm 13 µl 17 µl 30 µl
   Sample well 67.44 mm 310 µl 400 µl 680 µl
 
Specifications      
Number of gels 1–4 Total buffer volume for 2 gels 700 ml
Precast gels Mini-PROTEAN and Ready Gel Total buffer volume for 4 gels 1,000 ml
Handcast gels Cast using Mini-PROTEAN spacer plates Typical run times for SDS-PAGE 35–45 min (at 200 V constant)
Cassette size (W x L) Precast: 10 x 8.3 cm Recommended power supply PowerPac™ Basic or PowerPac HC
Glass plate size (W x L) Short plate: 10.1 x 7.3 cm Dimensions (W x L x H) 12 x 16 x 18 cm
  Spacer plate: 10.1 x 8.2 cm Weight 1 kg (2.2 lb)

The Mini-PROTEAN® Tetra handcast systems are based on the 4-gel Mini-PROTEAN Tetra cell or the high-throughput Mini-PROTEAN® 3 Dodeca™ cell (for running up to 12 gels). The accessories for hand casting provide options for casting mini gels in a variety of thicknesses and comb configurations, and even offer handy gel stands for help in casting gels.

Combs

A variety of comb styles are available, including 5-, 9-, 10-, and 15-well formats. The prep/2-D comb contains a reference well and the IPG comb is designed to accommodate a 7 cm ReadyStrip™ IPG strip. These combs fit both Mini-PROTEAN Tetra and Mini-PROTEAN 3 cells.

Plates for Hand Casting

To hand cast a gel, you need a short plate and a spacer plate. You do not need to order spacers separately; simply select the spacer plate that has the spacer width you need (spacer plates contain spacers already joined to the plate). The short plates and the spacer plates fit both Mini-PROTEAN Tetra and Mini-PROTEAN 3 cells.

Mini-PROTEAN 3 Multi-Casting Chamber

Use the Mini-PROTEAN 3 multi-casting chamber to simultaneously cast up to 12 gels of 0.75, 1.0, or 1.5 mm thickness. Acrylic blocks act as space fillers when fewer than 12 gels are cast. You can cast gradient gels through a bottom-filling port with the Model 485 gradient former to ensure reproducibility. Gels cast in the multi-casting chamber can be run on any of the Mini-PROTEAN electrophoresis systems, including the Mini-PROTEAN Tetra cell.

Mini-PROTEAN Tetra Cell #165-8000
Mini-PROTEAN® Tetra Cell

165-8000
4-gel vertical electrophoresis system, 0.75 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates

List Price:   $897.00
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Mini-PROTEAN® Tetra Cell

165-8001
4-gel vertical electrophoresis system, 1.0 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates

List Price:   $897.00
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Mini-PROTEAN® Tetra Cell

165-8002
2-gel vertical electrophoresis system, 0.75 mm gel thickness, includes casting stand, 2 casting frames, 10-well combs, 5 short plates, and 5 spacer plates

List Price:   $708.00
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Mini-PROTEAN® Tetra Cell

165-8003
2-gel vertical electrophoresis system, 1.0 mm gel thickness, includes casting stand, 2 casting frames, 10-well combs, 5 short plates, and 5 spacer plates

List Price:   $708.00
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Mini-PROTEAN® Tetra Cell

165-8006
4-gel vertical electrophoresis system, 1.5 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates

List Price:   $897.00
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Mini-PROTEAN® Tetra Cell

165-8007
2-gel vertical electrophoresis system, 1.5 mm gel thickness, 1 casting stand, 2 casting frames, 10-well combs, 5 sets of glass plates

List Price:   $708.00
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Mini-PROTEAN 3 Multi-Casting Chamber

165-4110
Pkg of 1, 12-gel polyacrylamide gel casting chamber, includes 8 acrylic blocks, 15 separation sheets, tapered luer connector, stopcock valve

List Price:   $393.00
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Mini-PROTEAN 3 Multi-Casting Chamber

165-4111
Pkg of 1, polyacrylamide gel casting chamber with 0.75 mm spacers, includes 15 sets of glass plates; order combs separately

List Price:   $550.00
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Mini-PROTEAN 3 Multi-Casting Chamber

165-4112
Pkg of 1, polyacrylamide gel casting chamber with 1.0 mm spacers, includes 15 sets of glass plates; order combs separately

List Price:   $550.00
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Mini-PROTEAN 3 Multi-Casting Chamber

165-4113
Pkg of 1, polyacrylamide gel casting chamber with 1.5 mm spacers, includes 15 sets of glass plates; order combs separately

List Price:   $550.00
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Acrylic Blocks

165-4114
Pkg of 8, 6 mm space filler, for use with Mini-PROTEAN 3 multi-casting chamber (#165-4110)

List Price:   $92.00
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Separation Sheets

165-4115
Pkg of 15, reusable sheet for separation of gel sandwiches after casting in the Mini-PROTEAN 3 multi-casting chamber (#165-4110)

List Price:   $58.00
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Gel Releasers

165-3320
Pkg of 5, gel releaser for Mini-PROTEAN Tetra system, Mini-PROTEAN 3 Dodeca cell, Mini-PROTEAN 3 multi-casting chamber, and PROTEAN Plus Dodeca cell

List Price:   $30.00
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Model 485 Gradient Former

165-4120
40–175 ml acrylamide gradient former, includes valve stem and tubing kit, for use with Mini-PROTEAN multi-casting chamber systems

List Price:   $784.00
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Model 485 Gradient Former and Mini-PROTEAN 3 Multi-Casting Chamber

165-4122
Gradient gel casting system, includes 40–175 ml acrylamide gradient former (#165-4120) and 12-gel casting chamber (#165-4110) with accessories

List Price:   $1,127.00
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Exponential Piston

165-2006
Pkg of 1, exponential piston for Model 385 and Model 485 gradient formers

List Price:   $276.00
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AnyGel™ Stand

165-4131
Pkg of 1, single-row gel stand, holds 1 PROTEAN® gel, 2 Criterion™ gels, or 3 Mini-PROTEAN® or Ready Gel® precast mini gels

List Price:   $73.00
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AnyGel™ Stand

165-5131
Pkg of 1, 6-row gel stand, holds 6 PROTEAN® gels, 12 Criterion™ gels, or 18 Mini-PROTEAN® or Ready Gel® precast mini gels

List Price:   $271.00
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Number Description Options
2621 PROTEAN Plus Dodeca Cell System Brochure, Rev C Click to download
10024259 Mini-PROTEAN Empty Cassette Gel Casting Instructions, Rev A Click to download
M4006192 Instruction Manual, Model 485 Gradient Former, Rev A Click to download
M4006193 Instruction Manual, Mini-PROTEAN 3 Multi-Casting Chamber, Rev A Click to download