Mini-PROTEAN® Tris/Tricine Precast Gels

Mini-PROTEAN® Tris/Tricine Precast Gels

Mini-PROTEAN Tris/tricine precast gels are ideal for the separation of peptides and small proteins with molecular weights <10 kD. These polyacrylamide gels improve resolution by slowing the migration rates of peptide-SDS complexes.

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Overview

Mini-PROTEAN Tris-Tricine precast gels are ideal for separation of peptides and small proteins with a molecular weight <10,000. Superior resolution is achieved by slowing the migration rate of the peptide-SDS complexes. This helps achieve separation from the faster-moving SDS micelles that interfere with peptide resolution in Tris-glycine buffer systems.

Mini-PROTEAN precast gels are designed for the traditional 8 x 10 cm mini vertical format. Proteins and nucleic acids can be separated by electrophoresis on Mini-PROTEAN precast gels using the Mini-PROTEAN® Tetra electrophoresis cell (1–4 gels) or, for high-throughput applications, the Mini-PROTEAN® 3 Dodeca™ cell (1–12 gels). Gel opening lever (456-0000), sold separately, is 100% aluminum and recyclable.

Specifications

Gel dimensions (W x L x thickness)
8.6 x 6.7 x 0.1 cm
Cassette dimensions (W x L x thickness)
10 x 8 x 0.46 cm
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Gel storage conditions
Store flat at 2–8°C; do not freeze
Shelf life at recommended temperature*
14 weeks
Opening lever
100% aluminum and recyclable
* From date of manufacture.
 

Ordering

items

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4563063
Pkg of 2, 16.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells
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4563065
Pkg of 2, 16.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells
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4563066
Pkg of 2, 16.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells

Accessories

Kits & Reagents

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1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610739
30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE, contains 200 mM Tris-HCl, pH 6.8, 40% glycerol, 2% SDS, 0.04% Coomassie Blue G-250
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1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
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1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
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1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications

Documents

Number Description Options
1658100
Mini-PROTEAN Precast Gels Instruction Manual and Application Guide
6052
Ready Gel® to Mini-PROTEAN® TBE, TBE-Urea, and Tris-Tricine Precast Gels Conversion Chart, Rev B
6062
Instruction Manual, Mini-PROTEAN Precast Gels Quick Start Guide, Rev C
10026448
Mini-PROTEAN Precast Gel Quick Start Guide, Rev C