Mini-PROTEAN  TGX Stain-Free Precast Gels

Mini-PROTEAN TGX Stain-Free Precast Gels

Mini-PROTEAN TGX Stain-Free precast gels for polyacrylamide gel electrophoresis (PAGE) allow for fast run times, high transfer efficiency, and rapid detection of proteins with stain-free enabled imagers.

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Overview

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stain free western blotting guide
 

What is Stain-Free Technology?

Download the Complete Guide to Stain-Free Western Blotting and learn how it will revolutionize your research.

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Mini-PROTEAN TGX Stain-Free Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.

Features and Benefits of Mini-PROTEAN TGX Stain-Free Precast Gels

  • Run up to 4 Mini-PROTEAN TGX Stain-Free Protein Gels using the Mini-PROTEAN Tetra Cell
  • Run times as short as 15 minutes
  • Transfer proteins in as little as 15 minutes; verify transfer to membrane with a stain-free enabled imager
  • Inexpensive Laemmli buffer system, low running costs
  • Gel images and complete analysis in less than 5 minutes after electrophoresis
  • Comparable sensitivity to Coomassie stain
  • Better reproducibility and quantitation compared to staining procedures

Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels

  • 1-D polyacrylamide gel electrophoresis for protein separation
  • Accurate molecular weight estimation
  • Robust system for complex samples
  • One gel for western blotting, staining, and mass spectrometry

More Information

Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.

Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.

The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.

Ready to make the switch to Stain-Free enabled precast gels? Use the Western Blot Selector Tool to easily find gels compatible for your next experiment.

Gel opening lever (4560000), sold separately, is 100% aluminum and recyclable

Ordering

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4568021

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells

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4568023

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568024

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568025

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568026

Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers

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4568031

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells

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4568033

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568034

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568035

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568036

Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568041

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568043

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers

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4568044

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568045

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568046

Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568081

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568083
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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17000546
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
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4568084

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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17000927

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards

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4568085

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568086

Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568091

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568093
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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17000928
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
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4568094

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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17000436

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards

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4568095

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568096

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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17000435

Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards

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4568101

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568103

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568104

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568105

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568106

Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568121

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568123

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568124

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

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4568125

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers

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4568126

Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

Accessories

Kits & Reagents

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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

Documents

Number Description Options
6427
Bio-Rad Stain-Free Western Blotting
[ Add to Cart (Free) ]
6356
Traditional vs. Stain-Free Western Blotting
10026447
Mini-PROTEAN® TGX™ Precast Gel Quick Start Guide, Rev C
5974
Mini-PROTEAN® and Criterion™ TGX Stain-Free™ Precast Gels Product Information Sheet, Rev E
5932
Ready Gel to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev E
5934
NuPAGE Bis-Tris Precast Gels (MOPS Buffer) to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev G
6040
Electrophoresis Guide, Interactive PDF, Rev C
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
6390
General Stain-Free Western Blotting Protocol
[ Add to Cart (Free) ]
6434
Western Blot Normalization Using Image Lab™ Software, Quick Start Guide
[ Add to Cart (Free) ]
6166
Multiplex Fluorescent Blot Detection: A Troubleshooting Guide
5910
Mini-PROTEAN TGX Precast Gel: A Gel for SDS-PAGE with Improved Stability — Comparison with Standard Laemmli Gels, Rev B
5810
Compatibility of the Criterion Stain-Free Gel Imaging System with Mass Spectrometric Protein Analysis
6453
Utilize Stain-Free Technology in a Variety of Workflows, Rev B
[ Add to Cart (Free) ]
6494
Protein Purification Workflow with the NGC Chromatography System and Stain-Free Gels Brochure
[ Add to Cart (Free) ]
6507
TGX and TGX Stain-Free Gels: Publications List