These reusable sealing pads are designed to adhere to a motorized heated lid. They are ideal for applications such as cycle sequencing, in which several successive runs may be sealed with the same pad. Use 'P' pads with the original version of Power Bonnet lids (catalog #s ALP-1296 and ALP-1238), and 'P+' pads with the newer-design Moto Alpha units (catalog #s ALP-2296 and ALP-2238) in DNA Engine thermal cyclers. Microseal 'P+' pads provide improved sealing of low-volume reactions — as low as 5 µl in 96-well plates and 1 µl in 384-well plates. Each pad may be used for approximately 25 PCR runs, and can be surface-treated for periodic cleaning and decontamination. They are autoclavable and moderately resistant to UV irradiation.
For reusable, automation-friendly sealers that can be applied and removed by robotic devices, see the auto-sealing lids for PCR plates. These metal lids use the Microseal 'P' or 'P+' material for sealing, and are ideal for reaction volumes down to 1 µl. Microseal 'P' and 'P+' pads are intended for use exclusively with the higher sealing pressures provided by a motorized lid.