MicroPulser™ Electroporator



Transfection Protocol Library

The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you. browse protocols to view our library and find your starting point.

Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years. Instruments, including the Gene Pulser® series of electroporators, the MicroPulser electroporator, and the Helios® gene gun and PDS-1000/He biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. These transfection instruments are cited extensively in the literature. Our chemically mediated methods include lipid-based transfection reagents — TransFectin lipid reagent for delivery of plasmid DNA and siLentFect lipid reagent for siRNA delivery.

The MicroPulser electroporator is a simple yet versatile instrument that enables safe and reproducible transformation of bacteria, yeast, and other microorganisms. Transformation efficiencies much higher than those obtained with chemical methods can be achieved.

Key Features

  • One-button pulse delivery, attached cuvette chamber, and rapid charge time for fast sample handling
  • Preset, optimized programs for commonly studied bacteria and fungi allow rapid program selection
  • Arc quenching (ARQ) system significantly reduces arcing, protecting against loss of valuable samples
  • Broad range of parameters for manual optimization. Manual programming allows voltage to be selected in a 200–3,000 V range with 10 V precision and allows pulse width to be selected in a 1.0–4.0 ms range with 0.1 ms precision
  • High-voltage 3,000 V capability yields improved efficiency in larger-volume cuvettes
  • Compact, space-saving design
  • Audible and visible pulse indicators
  • Time constant and actual volts displayed to monitor reproducibility.
Outputs Waveform: decaying or truncated decaying exponential-decay with resistor capacitor time constant
Voltage and current: 3,000 V peak into >600 W load; limited at 100 A peak maximum
Output voltage and pulse duration adjustment Voltage adjustable in 200–3,000 V range with 10 V precision; 5 ms default or 1–4 ms with 0.1 ms precision.
Input voltage 100–120 V or 220–240 V
Preset protocols 5 bacterial, 5 fungal
Operating environment 3.5–35°C
Dimensions (W x D x H) 29 x 21 x 8 cm
Weight 2.9 kg

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MicroPulser Electroporator

100–120 V/220–240 V, electroporation instrument, includes 10 sterile electroporation cuvettes (5 each of 0.1 cm and 0.2 cm electrode gap width)

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Number Description Options
5542 Electroporation Systems Brochure, Rev A Click to download [ Add to Cart (Free) ]
5448 MicroPulser Electroporator Flier, Rev A Click to download
2751 MicroPulser™ Electroporator Brochure, Rev A Click to download
6826 Publication List: Electroporation of Nucleic Acids and Proteins for CRISPR-Related Gene Editing, Ver A Click to download