Microbial Culturing Module

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Overview

Lab Preparation Checklist
Kit contains sufficient materials to pour 40 LB agar plates (with or without ampicillin), inoculate and grow 75 miniprep cultures, and contains starter E. coli bacteria.
Ampicillin, lyophilized 2
LB broth capsules 12
LB nutrient agar powder 1
Petri dishes, 60 mm, sterile 40
Cell culture tubes, 15 ml, sterile 75
Inoculation loops, sterile 80
E. coli strain HB101 K-12, lyophilized 1
Disposable plastic transfer pipets 10
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Microwave oven or autoclave  
Recommended (Optional) Accessories:
Incubation oven
Shaking water bath
Microbial Culturing

Microbial Culturing: As part of the Cloning and Sequencing Explorer Series.

Microbial Culturing

Select for positive ligations and grow bacterial minipreps. Students use this module to prepare media for their microbiological needs for the Cloning and Sequencing Explorer Series.

Starter cultures are required for competent cell preparation. Students will make LB agar plates and streak them with E. coli bacteria for single colonies. Students then prepare LB broth to make an overnight starter culture from their streaked plate, which is used to prepare competent cells using the ligation and transformation module.

LB ampicillin agar plates are required to select for bacteria that have been transformed with plasmids — like pJET1.2 — which contain the Β-lactamase gene conferring ampicillin resistance to bacteria. After transformation, bacteria are plated onto LB ampicillin agar plates and incubated at 37°C and ampicillin resistant bacterial colonies will grow overnight.

LB broth containing ampicillin is required to culture minipreps grown from colonies transformed with ligated plasmid. Plasmids are then isolated from the minipreps using the Aurum plasmid mini purification kit.

The microbial culturing module contains the reagents necessary for teaching basic sterile technique, culturing E. coli, transforming ampicillin-resistant bacteria, and growing miniprep cultures. The protocol can easily be integrated into existing microbiology laboratory activities and independent study projects.

This module is an integral component of the Cloning and Sequencing Explorer Series but may also be purchased separately for preparing media for bacterial culture propagation.

Kit contains sufficient materails to pour 40 LB agar plates (with or without ampicillin), inoculate and grow 75 miniprep cultures, and contains starter E. coli bacteria  
Vials of ampicillin  2         
LB broth capsules 12         
LB nutrient agar powder 1         
Petri dishes, 60 mm, sterile 40         
Cell culture tubes, 15 ml, sterile 75         
Inoculation loops, sterile 80         
E. coli strain HB101 K-12, lyophilized 1         
Disposable plastic transfer pipets 10         
Instruction manual 1         
Microbial Culturing Module #166-5020EDU
Microbial Culturing Module

166-5020EDU
Sixth module in Cloning and Sequencing Explorer Series classroom study kit, includes ampicillin, petri dishes, E. coli strain HB101 K-12, curriculum, and more, for 12 workstations; education use only

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EDU Price:   $96.00   What is this?
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Number Description Options
1665022 Instruction Manual, Microbial Culturing Kit, Biotechnology Explorer, Rev A Click to download