Macro-Prep® Weak Cation Exchange Media

Print

Overview

Specifications
Property DEAE High Q High S CM
Type of support Weak anion Strong anion Strong cation Weak cation
Functional ligand –N+(C2H5)2 –N+(CH3)3 –SO3- –COO-
Ionic capacity 175 ±75 µeq/ml 400 ±75 µeq/ml 160 ±40 µeq/ml 210 ±40 µeq/ml
Typical binding capacity 35 mg BSA/ml 40 mg BSA/ml 70 mg IgG/ml 35 mg hemoglobin/ml
Counterion Cl- Cl- Na+ Na+
Nominal particle size 50 µm 50 µm 50 µm 50 µm
Nominal pore size 1,000 Å 1,000 Å 1,000 Å 1,000 Å
Recommended maximum linear flow rate 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr
Chemical stability        
   1% SDS, 24 hr Yes Yes Yes Yes
   6 M guanidine-HCl, 24 hr Yes Yes Yes Yes
Volume changes        
   pH 4–10 <1% <1% <3% <1%
   0.1–1.0 M NaCl <5% <5% <9% <4%
Autoclavability* (121°C, 80 min) Yes Yes Yes Yes
pH stability 1–10 1–10 1–12 1–12
Antimicrobial agent 20% ethanol 20% ethanol 20% ethanol 20% ethanol
Regeneration 1 M NaCl 1 M NaCl 1 M NaCl 1 M NaCl

* Do not autoclave the OH- form.

Product Selection by Technique
   
Scale
Packaging Format
Characteristics Product Type Analytical Pilot/Preparative Process Prepacked Bottled
Ion Exchange Chromatography
Separation by charge UNOsphere™ media     UNOsphere Q, S, Rapid S
General biomolecules purification Macro-Prep® media     Macro-Prep High Q, DEAE, High S, CM
Applicable to all antibody purifications Macro-Prep 25 media     Macro-Prep 25 Q, 25 S
UNO® columns   UNO Q, S  
Bio-Scale™ Mini Cartridges     UNOsphere Q, S  
High capacity         Macro-Prep High Q, S  
Good capture technique AG® resins   AG
Chelex® resins Chelex
Bio-Rex® resins Bio-Rex
Hydroxyapatite and Fluoroapatite Chromatography
Unique selectivity CHT™ 80 µm support     CHT, Types I and II
Alternative to protein A for antibody purification CHT 40 µm support     CHT, Types I and II
CHT 20 µm support   CHT, Types I and II
CFT 40 µm support CFT, Types I and II
Removal of leached protein A, aggregates, endotoxins, and DNA Bio-Gel® HT and HTP media     Bio-Gel HT, HTP
Bio-Scale columns   CHT Type I (10 µm)  
CHT Type II (20 µm)  
Affinity Chromatography
Purification of His- and GST- tagged proteins Profinity™ IMAC and GST media   Bio-Scale Mini Profinity IMAC
Separation by biological interaction Affi-Gel resins     Affi-Gel® Protein A*
Affi-Gel Blue*
DEAE Affi-Gel Blue*
CM Affi-Gel Blue*
High selectivity           Affi-Gel boronate, heparin**
High purity in a single step           Affi-Gel Hz, 10, 15**
Affi-Gel 102**
Excellent capture technique Profinity epoxide media     Profinity epoxide**
Affi-Prep media   Affi-Prep protein A*
Affi-Prep polymyxin*
Bio-Scale Mini cartridges   Profinity IMAC and GST  
Econo-Pac® columns   Affi-Gel Blue  
DEAE Affi-Gel Blue
Affi-Gel protein A
Size Exclusion Chromatography
Separation by size Bio-Gel P media     Bio-Gel P
MW determination Bio-Beads™ S-X media     Bio-Beads S-X
Applicable to all antibody purifications Bio-Sil® and Bio-Silect™ columns     Bio-Sil, Bio-Silect  
Desalting or buffer exchange Bio-Scale Mini cartridges     Bio-Gel P-6  
High resolution Econo-Pac columns     Econo-Pac 10DG  
Hydrophobic Interaction Chromatography
Separation by hydrophobicity Macro-Prep HIC media     Macro-Prep t-butly
Macro-Prep methyl

* Ready to use.
** Activated.

Common Buffers for Ion Exchange
Type of Ion Exchanger Buffer Buffering Range
Cation Acetic acid 4.8–5.2
  Citric acid 4.2–5.2
  HEPES 6.8–8.2
  Lactic acid 3.6–4.3
  MES 5.5–6.7
  MOPS 6.5–7.9
  Phosphate 6.7–7.6
  PIPES 6.1–7.5
  TES 7.2–7.8
  Tricine 7.8–8.9
Anion Bicine 7.6–9.0
  Bis-Tris 5.8–7.2
  Diethanolamine 8.4–8.8
  Diethylamine 9.5–11.5
  L-histidine 5.5–6.0
  Imidazole 6.6–7.1
  Pyridine 4.9–5.6
  Tricine 7.4–8.8
  Triethanolamine 7.3–8.3
  Tris 7.5–8.0

Note:Buffer solution of 50 mM is recommended.

Macro-Prep CM weak cation exchange support meets the demands of analytical, semipreparative, and process-scale applications. This rigid, macroporous hydrophilic support provides exceptional capacity, resolution, and throughput in a chemically and mechanically stable form. Macro-Prep CM operates well at low and medium pressures and exhibits minimal volume changes in response to variations in pH or ionic strength. It provides consistent and reproducible results. Advantages of Macro-Prep CM support include:

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • Chemical, mechanical, and thermal stability

 

Macro-Prep CM Support

158-0070
25 ml, weak cation exchange chromatography media, 50 μm particle size

List Price:   $54.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Macro-Prep CM Support

156-0070
100 ml, weak cation exchange chromatography media, 50 μm particle size

List Price:   $161.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Macro-Prep CM Support

156-0071
500 ml, weak cation exchange chromatography media, 50 μm particle size

List Price:   $600.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Macro-Prep CM Support

156-0072
5 L, weak cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
Macro-Prep CM Support

156-0073
10 L, weak cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
Number Description Options
2292 Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A Click to download
LIT271 Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D Click to download
4006129 Instruction Manual, Macro-Prep 25 Ion Exchange Supports, Rev B Click to download