Macro-Prep® t-Butyl HIC Resin

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Macro-Prep® t-Butyl HIC Resin

Overview

Chemical Structure of Macro-Prep HIC Media

Macro-Prep HIC: Macro-Prep t-butyl contains t-butyl and carboxyl groups; Macro-Prep methyl contains methyl and carboxyl groups.

Macro-Prep HIC Media
  Retention Times (min)
PeakProteinMethylt-Butyl
1Cytochrome c7.310.41
2Ovalbumin32.2540.22
3Α-Anylase62.8171.5
4Ferritin81.4990.9

Comparison of retention times using Macro-Prep methyl and t-butyl ligands.

Macro-Prep HIC Dynamic Binding Capacity

Macro-Prep HIC supports can be sanitized extensively with .15% peracetic acid.

Retention Times of Macro-Prep HIC Media

Macro-Prep HIC supports can be sanitized extensively with .15% peracetic acid.

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Macro-Prep Hydrophobic Interaction Chromatography (HIC) Resins are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification. They are designed specifically for intermediate purification steps that remove host-cell contaminants from partially purified targets. Due to their rigidity and unique surface chemistry, these hydrophobic chromatography resins are particularly suited for HIC operations requiring high throughput and high recovery of target.

Macro-Prep HIC Resin is available in two functional forms:

  • Macro-Prep Methyl HIC Resin — weakly hydrophobic, for purifying compounds that have strong hydrophobic regions
  • Macro-Prep t-Butyl HIC Resin — mildly hydrophobic, for purifying compounds that have few or weakly hydrophobic regions
Both HIC resins are chemically and thermally stable. These media are autoclavable and can withstand treatment in acid, chaotropic agents, or detergents while retaining high protein-binding capacities.

Macro-Prep t-Butyl HIC Resin operates on an interaction mechanism based on hydrophobicity and charge. The t-butyl groups are mildly hydrophobic. Depending on the pH of loading and elution buffers, the carboxyl groups can be exploited to ionically repel target molecules while the hydrophobic groups retain contaminants. This is an ideal strategy that minimizes the product loss commonly experienced with HIC media due to denaturation of proteins upon exposure to hydrophobic surfaces.

More Information

Macro-Prep® HIC Resin Product Information Sheet (Bulletin 1841)

Instruction Manual, Macro-Prep® HIC Resins (LIT486)

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

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Macro-Prep t-Butyl HIC Support

1580090
25 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

3 1580090 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/158-0090_0921120652849.xml 1580090 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:03 1580090-macro-prep-t-butyl-hic-support 1580090 Macro-Prep t-Butyl HIC Support Macro-Prep t-Butyl HIC Support 25 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 25 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 25 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size /webroot/web/images/lsr/products/chromatography/sku_thumb/global/158-0090_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/158-0090_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/158-0090_feature.jpg RepEx 158-0090 25 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1580090, macro-prep, t-butyl, hic, support, hydrophobic, interaction, chromatography, media, medium, resin, protein, separation, purification, 50um, 50, particle, size false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1580090&CartQuote=QT&quantity= false true false $116.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1580090&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1580090&quantity= 1580090
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Macro-Prep t-Butyl HIC Support

1560090
100 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

4 1560090 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0090_0921120652830.xml 1560090 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1560090-macro-prep-t-butyl-hic-support 1560090 Macro-Prep t-Butyl HIC Support Macro-Prep t-Butyl HIC Support 100 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 100 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 100 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0090_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0090_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0090_feature.jpg RepEx 156-0090 100 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1560090, macro-prep, t-butyl, hic, support, hydrophobic, interaction, chromatography, media, medium, resin, protein, separation, purification, 50um, 50, particle, size false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560090&CartQuote=QT&quantity= false true false $431.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560090&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560090&quantity= 1560090
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Macro-Prep t-Butyl HIC Support

1560091
500 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

5 1560091 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0091_0921120652876.xml 1560091 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1560091-macro-prep-t-butyl-hic-support 1560091 Macro-Prep t-Butyl HIC Support Macro-Prep t-Butyl HIC Support 500 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 500 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 500 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size /webroot/web/images/ps/products/hydro_interaction_chrom_media/sku_thumb/global/156-0091_tn.jpg /webroot/web/images/ps/products/hydro_interaction_chrom_media/sku_view/global/156-0091_view.jpg /webroot/web/images/ps/products/hydro_interaction_chrom_media/sku_feature/global/156-0091_feature.jpg RepEx 156-0091 500 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1560091, macro-prep, t-butyl, hic, support, hydrophobic, interaction, chromatography, media, medium, resin, protein, separation, purification, 50um, 50, particle, size false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560091&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560091&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560091&quantity= 1560091
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(Discontinued)Macro-Prep t-Butyl HIC Support

156-0092
5 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
Macro-Prep t-Butyl HIC Support

156-0093
10 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

7 1560093 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0093_0921120652919.xml 1560093 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1560093-macro-prep-t-butyl-hic-support 1560093 Macro-Prep t-Butyl HIC Support Macro-Prep t-Butyl HIC Support 10 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 10 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 10 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size /webroot/web/images/ps/products/hydro_interaction_chrom_media/sku_thumb/global/156-0093_tn.jpg /webroot/web/images/ps/products/hydro_interaction_chrom_media/sku_view/global/156-0093_view.jpg /webroot/web/images/ps/products/hydro_interaction_chrom_media/sku_feature/global/156-0093_feature.jpg RepEx 156-0093 10 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1560093, macro-prep, t-butyl, hic, support, hydrophobic, interaction, chromatography, media, medium, resin, protein, separation, purification, 50um, 50, particle, size, analytical, semi-preparative, process, scale false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560093&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560093&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560093&quantity= 156-0093
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Media Sampler Pack

1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm

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5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm Includes 25 ml each of Macro-Prep High Q, High S, methyl HIC, and t-butyl HIC; 25 ml each of UNOsphere Q and S; 10 g of CHT Type II; 10 g of CFT Type II /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/158-0100_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/158-0100_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/158-0100_feature.jpg Media Sampler Pack #158-0100 preview RepEx Media Sampler Pack #158-0100 Includes 25 ml each of Macro-Prep<sup>®</sup> High Q, High S, DEAE, 25 ml of Nuvia™ S, 25 ml each of UNOsphere™ Q and S, 5 ml of UNOsphere SUPrA™, 10 g of CFT™ Type II, 10 g of CHT™ Types I & II 1580100, media, sampler, pack, assorted, macro-prep, high, q, s, deae, nuvia, unosphere, supra, cft, type, ii, 10 g of cht, types, i and ii, chromatography, medium, resin, protein, separation, purification 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1580100&CartQuote=QT&quantity= false true false $711.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1580100&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1580100&quantity= 1580100
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Number Description Options
LIT486 Instruction Manual, Macro-Prep® HIC Supports, Rev C Click to download
1841 Macro-Prep® HIC Resin Product Information Sheet, Ver F Click to download
6792 Aggregate Purification Resin Selection Card, Ver A Click to download
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download
6808 Practical Guide: Selecting the Optimal Resins for Aggregate Removal, Ver A Click to download
6810 Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A Click to download
6868 Macro-Prep® Chromatography Resins Publications List, Ver A Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download

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