Macro-Prep Methyl HIC Media

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Overview

Chemical Structure of Macro-Prep HIC Media

Macro-Prep HIC: Macro-Prep t-butyl contains t-butyl and carboxyl groups; Macro-Prep methyl contains methyl and carboxyl groups.

Pressure vs. Flow Data

Pressure vs. flow for Macro-Prep media. Media bed dimensions were 140 mm diameter by 250 mm length.

Macro-Prep HIC Media
  Retention Times (min)
PeakProteinMethylt-Butyl
1Cytochrome c7.310.41
2Ovalbumin32.2540.22
3Α-Anylase62.8171.5
4Ferritin81.4990.9

Comparison of retention times using Macro-Prep methyl and t-butyl ligands.

Macro-Prep HIC Dynamic Binding Capacity

Macro-Prep HIC supports can be sanitized extensively with .15% peracetic acid.

Retention Times of Macro-Prep HIC Media

Macro-Prep HIC supports can be sanitized extensively with .15% peracetic acid.

Macro-Prep hydrophobic interaction chromatography (HIC) media are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification. They are designed specifically for intermediate purification steps that remove host-cell contaminants from partially purified targets. Due to their rigidity and unique surface chemistry, these media are particularly suited for HIC operations requiring high throughput and high recovery of target.

These media are available in two functional forms:

  • Macro-Prep methyl HIC support — weakly hydrophobic, for purifying compounds that have strong hydrophobic regions
  • Macro-Prep t-butyl HIC support — mildly hydrophobic, for purifying compounds that have few or weakly hydrophobic regions

Both media are chemically and thermally stable. They are autoclavable and can withstand treatment in acid, chaotropic agents, or detergents while retaining high protein-binding capacities.

Macro-Prep methyl HIC support operates on a mechanism of interaction that is based on hydrophobicity and charge. The methyl groups are mildly hydrophobic. Depending on the pH of loading and elution buffers, the carboxyl groups can be exploited to ionically repel target molecules while the hydrophobic groups retain contaminants. This is an ideal strategy that minimizes the product loss commonly experienced with HIC media due to denaturation of proteins upon exposure to hydrophobic surfaces.

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Macro-Prep Methyl HIC Support

158-0080
25 ml, methyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $98.00
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Macro-Prep Methyl HIC Support

156-0080
100 ml, methyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $369.00
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Macro-Prep Methyl HIC Support

156-0081
500 ml, methyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $1,425.00
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Macro-Prep Methyl HIC Support

156-0082
5 L, methyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $4,000.00
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Macro-Prep Methyl HIC Support

156-0083
10 L, methyl hydrophobic interaction chromatography media, 50 μm particle size

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Number Description Options
1841 Macro-Prep HIC Support Product Information Sheet, Rev E Click to download
LIT486 Instruction Manual, Macro-Prep HIC Supports, Rev C Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download