Macro-Prep High Q Media

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Overview

Superior Resolution Separations at High Flow Rate

Macro-Prep High Q media: Effect of flow rate on peak symmetry and separation. A 5 ml sample (4.4 mg/ml) of myoglobin (peak 1), ovalbumin (peak 2), and BSA (peak 3) was run on a 1 x 13 cm (8.7 ml) column at each of the indicated flow rates. The buffer was 50 mM Tris-HCl, pH 8.6, with a gradient of 0–0.5 M NaCl over 100 ml.

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Macro-Prep High Q Media Dynamic Binding Capacity
Dynamic protein binding capacity as a function of linear flow rate

Dynamic protein binding capacity as a function of linear flow rate. A 100 ml sample (3 mg/ml) of BSA was run on a 1 x 10 cm (8 ml) column at the indicated flow rates. The buffer was 50 mM Tris-HCI, pH 8.3. Elution was with 1.0 M NaCl.

PDF Bulletin 5644 To read more click here

Macro-Prep High Q strong anion exchange support contains quaternary amine functional groups and is ideal for purifying acidic and neutral proteins and peptides. It is an excellent choice for rapid purification and meets the demands of analytical, semipreparative, and process-scale applications.

This methacrylate copolymer bead provides high-resolution separations at very high flow rates. Changes in pH or ionic strength of the buffer do not cause shrinking or swelling of the support. The superior mechanical and chemical stability of Macro-Prep High Q support make it a preferred choice over other Q supports on the market. Macro-Prep High Q support can be sanitized with chaotropic agents and can be autoclaved. It provides consistent and reproducible results.

Advantages of Macro-Prep High Q include:

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • High chemical, mechanical, and thermal stability

For more information, request bulletin 5644.

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Properties of Macro-Prep High Q Support  
Type of support Strong anion exchanger
Functional group –N+(CH3)3
Typical dynamic binding capacity ≥37 BSA/ml
Nominal particle size 50 µm
Nominal pore diameter 1,000 Å
Recommended maximum linear flow rate 3,000 cm/hr
pH stability 1–10
Regeneration in 1–2 M NaCl/KCl or 70% ethanol
Chemical Compatibility of Macro-Prep High Q Support  
1% SDS Yes
8 M guanidine-HCI Yes
1 N HCI Yes
100% ethanol Yes
Macro-Prep High Q Support #156-0040
Macro-Prep High Q Support

158-0040
25 ml, strong anion exchange chromatography media, 50 μm particle size

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Macro-Prep High Q Support #156-0041
Macro-Prep High Q Support

156-0040
100 ml, strong anion exchange chromatography media, 50 μm particle size

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Macro-Prep High Q Support #156-0042
Macro-Prep High Q Support

156-0041
500 ml, strong anion exchange chromatography media, 50 μm particle size

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Macro-Prep High Q Support #156-0043
Macro-Prep High Q Support

156-0042
5 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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Macro-Prep High S Support #156-0030
Macro-Prep High Q Support

156-0043
10 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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Number Description Options
5644 Macro-Prep High Q Support Bulletin, Rev A Click to download [ Add to Cart (Free) ]
1985 Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B. Click to download [ Add to Cart (Free) ]
1913 Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Supports (Poster), Rev B Click to download
LIT271 Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
2204 Effective Removal of Negatively Charged Interfering Molecules From Proteins, Rev B Click to download [ Add to Cart (Free) ]