Macro-Prep HIC Media

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Overview

Specifications
  Methyl HIC Support t-Butyl HIC Support
Functional ligand –CH3 –C(CH3)3
Binding capacity* >25 mg/ml >15 mg/ml
Nominal particle size 50 µm 50 µm
Recommended maximum linear flow rate 3,000 cm/hr 3,000 cm/hr
Autoclavability (121°C, 30 min) Yes Yes
pH stability 1–10 1–10
Regeneration 70% ethanol 70% ethanol
Storage 20% ethanol 20% ethanol

*Determined with human serum albumin.

Product Selection by Technique
   
Scale
Packaging Format
Characteristics Product Type Analytical Pilot/Preparative Process Prepacked Bottled
Ion Exchange Chromatography
Separation by charge UNOsphere™ media     UNOsphere Q, S, Rapid S
General biomolecules purification Macro-Prep® media     Macro-Prep High Q, DEAE, High S, CM
Applicable to all antibody purifications Macro-Prep 25 media     Macro-Prep 25 Q, 25 S
UNO® columns   UNO Q, S  
Bio-Scale™ Mini Cartridges     UNOsphere Q, S  
High capacity         Macro-Prep High Q, S  
Good capture technique AG® resins   AG
Chelex® resins Chelex
Bio-Rex® resins Bio-Rex
Hydroxyapatite and Fluoroapatite Chromatography
Unique selectivity CHT™ 80 µm support     CHT, Types I and II
Alternative to protein A for antibody purification CHT 40 µm support     CHT, Types I and II
CHT 20 µm support   CHT, Types I and II
CFT 40 µm support CFT, Types I and II
Removal of leached protein A, aggregates, endotoxins, and DNA Bio-Gel® HT and HTP media     Bio-Gel HT, HTP
Bio-Scale columns   CHT Type I (10 µm)  
CHT Type II (20 µm)  
Affinity Chromatography
Purification of His- and GST- tagged proteins Profinity™ IMAC and GST media   Bio-Scale Mini Profinity IMAC
Separation by biological interaction Affi-Gel resins     Affi-Gel® Protein A*
Affi-Gel Blue*
DEAE Affi-Gel Blue*
CM Affi-Gel Blue*
High selectivity           Affi-Gel boronate, heparin**
High purity in a single step           Affi-Gel Hz, 10, 15**
Affi-Gel 102**
Excellent capture technique Profinity epoxide media     Profinity epoxide**
Affi-Prep media   Affi-Prep protein A*
Affi-Prep polymyxin*
Bio-Scale Mini cartridges   Profinity IMAC and GST  
Econo-Pac® columns   Affi-Gel Blue  
DEAE Affi-Gel Blue
Affi-Gel protein A
Size Exclusion Chromatography
Separation by size Bio-Gel P media     Bio-Gel P
MW determination Bio-Beads™ S-X media     Bio-Beads S-X
Applicable to all antibody purifications Bio-Sil® and Bio-Silect™ columns     Bio-Sil, Bio-Silect  
Desalting or buffer exchange Bio-Scale Mini cartridges     Bio-Gel P-6  
High resolution Econo-Pac columns     Econo-Pac 10DG  
Hydrophobic Interaction Chromatography
Separation by hydrophobicity Macro-Prep HIC media     Macro-Prep t-butly
Macro-Prep methyl

* Ready to use.
** Activated.

Macro-Prep Resin
Macro-Prep HIC: Macro-Prep t-butyl contains t-butyl and carboxyl groups; Macro-Prep methyl contains methyl and carboxyl groups.
Common Buffers for Macro-Prep HIC
Buffer Useful pH Range
Citric acid 2.6–3.6
Lactic acid 3.6–4.3
Formic acid 3.8–4.3
Acetic acid 4.8–5.2
MES 5.5–6.7
PIPES 6.1–7.5
MOPS 6.5–7.9
Phosphate 6.7–7.6
Bicine 8.2–8.7
HEPES 6.8–8.2
TES 6.8–8.2
Tricine 7.4–8.8

Macro-Prep hydrophobic interaction chromatography (HIC) media are methacrylate-based 50 µm beads for protein, polypeptide, enzyme, and nucleic acid purification. These media are available in two functional forms:

  • The weakly hydrophobic methyl support is ideal for purifying compounds that have strong hydrophobic regions
  • The mildly hydrophobic t-butyl support is ideal for purifying compounds that have few or weakly hydrophobic regions.

Both media are chemically and thermally stable. They are autoclavable and can withstand treatment in acid, base, chaotropic agents, or detergents while retaining high protein binding capacities. They can be cleaned in place with ethanol or sodium hydroxide.

Macro-Prep Methyl HIC Support

158-0080
25 ml, methyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $98.00
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Macro-Prep Methyl HIC Support

156-0080
100 ml, methyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $369.00
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Macro-Prep Methyl HIC Support

156-0081
500 ml, methyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $1,425.00
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Macro-Prep Methyl HIC Support

156-0082
5 L, methyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $4,000.00
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Macro-Prep Methyl HIC Support

156-0083
10 L, methyl hydrophobic interaction chromatography media, 50 μm particle size

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Macro-Prep t-Butyl HIC Support

158-0090
25 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $98.00
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Macro-Prep t-Butyl HIC Support

156-0090
100 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $369.00
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Macro-Prep t-Butyl HIC Support

156-0091
500 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $1,425.00
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Macro-Prep t-Butyl HIC Support

156-0092
5 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

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Macro-Prep t-Butyl HIC Support

156-0093
10 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

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Number Description Options
1841 Macro-Prep HIC Support Product Information Sheet, Rev E Click to download
LIT486 Instruction Manual, Macro-Prep HIC Supports, Rev C Click to download [ Add to Cart (Free) ]