Macro-Prep® Anion Exchange Resin

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Macro-Prep Anion Exchange Resin - Anion Exchange Resin

Overview

Specifications
Property DEAE High Q High S CM
Type of support Weak anion Strong anion Strong cation Weak cation
Functional ligand –N+(C2H5)2 –N+(CH3)3 –SO3- –COO-
Ionic capacity 175 ±75 µeq/ml 400 ±75 µeq/ml 160 ±40 µeq/ml 210 ±40 µeq/ml
Typcial binding capacity 35 mg BSA/ml 40 mg BSA/ml 70 mg IgG*/ml 35 mg hemoglobin/ml
Counterion Cl- Cl- NA+ NA+
Nominal particle size 50 µm 50 µm 50 µm 50 µm
Nominal pore size 1,000 Å 1,000 Å 1,000 Å 1,000 Å
Recommended maximum linear flow rate 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr
Chemical stability
    1% SDS, 24 hr Yes Yes Yes Yes
    6 M guanidine-HCl, 24 hr Yes Yes Yes Yes
Volume changes
    pH 4–10 <1% <1% <3% <1%
    0.1–1.0 M NaCl <5% <5% <9% <4%
Autoclavability
(121°C, 80 min)
Yes Yes Yes Yes
pH stability 1–10 1–10 1–12 1–12
Antimicrobial agent 20% ethanol 20% ethanol 20% ethanol 20% ethanol
Regeneration 1 M NaCl 1 M NaCl 1 M NaCl 1 M NaCl

* Human IgG.

Macro-Prep High Q strong anion exchange support meets the demands of analytical, semipreparative, and process-scale applications. This rigid, macroporous hydrophilic support provides exceptional capacity, resolution, and throughput in a chemically and mechanically stable form.

All Macro-Prep media operate well at low and medium pressures and exhibit minimal volume changes in response to variations in pH or ionic strength. These media provide consistent and reproducible results.

Key Features

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • Chemical, mechanical, and thermal stability

 

Macro-Prep High Q Support #156-0040
Macro-Prep High Q Support

158-0040
25 ml, strong anion exchange chromatography media, 50 μm particle size

List Price:   $46.00
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Macro-Prep High Q Support #156-0041
Macro-Prep High Q Support

156-0040
100 ml, strong anion exchange chromatography media, 50 μm particle size

List Price:   $143.00
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Macro-Prep High Q Support #156-0042
Macro-Prep High Q Support

156-0041
500 ml, strong anion exchange chromatography media, 50 μm particle size

List Price:   $600.00
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Macro-Prep High Q Support #156-0043
Macro-Prep High Q Support

156-0042
5 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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Macro-Prep High S Support #156-0030
Macro-Prep High Q Support

156-0043
10 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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Number Description Options
2204 Effective Removal of Negatively Charged Interfering Molecules From Proteins, Rev B Click to download [ Add to Cart (Free) ]
1985 Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B. Click to download [ Add to Cart (Free) ]
5644 Macro-Prep High Q Support Bulletin, Rev A Click to download [ Add to Cart (Free) ]
2292 Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A Click to download
LIT271 Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D Click to download
6530 Protein Purification Workflow Development Using Bio-Rad’s NGC Chromatography System, Rev B Click to download