Macro-Prep 25 S Resin

Macro-Prep 25 S Resin

Overview

Macro-Prep 25 S strong cation exchange support offers high-resolution separations at high flow rates with medium pressures.
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Description

Description

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Macro-Prep 25 S Resin is a strong cation exchange resin that offers high-resolution separations at high flow rates with medium pressures. It possesses the same rigid, macroporous, and hydrophilic properties of 50 µm Macro-Prep High S support but in a 25 µm bead that offers higher resolution.

Features and Benefits

  • Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
  • Fewer chromatographic steps required
  • Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation

More Information

Instruction Manual, Macro-Prep 25 Resins (Bulletin 4006129)

Specifications

Specifications

Type of ion exchanger
Strong cation
Functional group
–SO3-
Total ionic capacity
110±30 µeq/ml
Median particle size
25 µm
Nominal pore size
725 Å
Shipping counterion
NA+
Dynamic binding capacity
>40 mg/ml lgG
Recommended linear flow rate range
50–300 cm/hr
Maximum linear flow rate
3,000 cm/hr
Chemical stability
 
  1.0 M HCI
>72 hr
  100% ethanol
Excellent
  Chaotropic agents (8 M urea, 6 M guanidine-HCI)
Excellent
pH stability
1–12
Volume changes
 
  ph 4–10
<1%
  0.1–1.0 M Na Cl
<4%
Autoclavability (121°C, 30 min)
Yes
Storage solution
20% ethanol

Ordering

Ordering

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1530032
200 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

Kits & Reagents

Kits & Reagents

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1250562
Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography

Documents

Documents

Number Description Options
2292
Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A
4006129
Instruction Manual, Macro-Prep 25 Ion Exchange Resins, Ver C
5662
Process Chromatography Applications Laboratory Brochure, Rev B