Ligation and Transformation Module

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Ligation and Transformation Module

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 12 workstations.
T4 DNA ligase, 10 µl 1
2x ligase buffer, 100 µl 1
Proofreading DNA polymerase, 10 µl 1
Cloning vector, 10 µl 1
Bgl II restriction enzyme, 50 µl 1
10x restriction enzyme buffer, 100 µl 1
C-Growth medium, 20 ml 1
Transformation A solution, 5 ml 1
Transformation B solution, 5 ml 1
IPTG, 0.1 ml 1
Sterile water, 2.5 ml 1
Colored micro test tubes, 2.0 ml 120
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Water bath
Microcentrifuge
Incubation oven
Shaking water bath

Note: Bacterial culturing reagents such as LB broth and LB agar are contained in the Microbial Culturing module.

Ligation and Transformation

Ligate the PCR product into a plasmid and transform bacteria. Students directly clone their PCR products into the pJET1.2 vector and immediately transform bacteria using a protocol that takes less than 2 hours to go from purified PCR product to transformed bacteria plated on agar.

Ligation and Transformation

Students first remove the 3'-dA overhang from their PCR product which results from amplification with Taq polymerase using a proofreading polymerase. A 10 minute protocol is then used to ligate the blunted PCR product into a pre-opened and blunted vector — pJET1.2. The pJET1.2 plasmid positively selects for successful insertion of fragments because the cloned fragment inserts into a lethal gene present in the plasmid that prevents its activation. Bacteria that are transformed with re-ligated vector activate the gene and are killed. This results in higher transformation efficiency than traditional blue-white cloning. Bgl II restriction sites are located on either side of the pJET1.2 cloning site, which allow students to determine if their gene of interest was successfully ligated once they have isolated candidate plasmids.

To transform the ligation into bacteria, students make competent cells by inoculating specialized growth media and performing a series of microcentrifugations and washes in a specialized transformation buffer. Competent cells are then added to the ligations on ice and plated directly on warm agar plates. Between 10 and 100 colonies should be expected from transformed ligations with the Cloning and Sequencing Explorer Series.

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The ligation and transformation module is an integral component of the Cloning and Sequencing Explorer Series. Use this module to directly ligate PCR products into the pJET1.2 plasmid vector and immediately transform freshly prepared competent bacteria with the ligation reaction. Competent cells are then plated directly on warm agar plates and incubated overnight at 37°C.

Once transformed bacteria have been propagated, plasmid DNA can be isolated and digested with BglII enzyme. The BglII restriction sites located on either side of the pJET1.2 cloning site allow students to determine if their PCR product was successfully ligated.

This module may also be purchased separately and used to clone any PCR product. The entire ligation and transformation protocol takes approximately 2 hours to complete and does not require commercial competent cells, a refrigerated microcentrifuge, or a –80°C freezer, making it the method of choice for educators worldwide.

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Ligation And Transformation Module

1665015EDU
Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only

0 1665015EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5015EDU_0921121012507.xml 1665015EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcrucx0hjcrucx0i" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcrucx0hjcrucx0i"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="23" DayOfWeek="2" Year="2018" Hour="21" Minute="25" Second="10" ZoneOffsetMillis="0" Time="1516742710939">Tue Jan 23 21:25:10 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5015EDU_0921121012507.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="23" DayOfWeek="2" Year="2018" Hour="21" Minute="25" Second="10" ZoneOffsetMillis="0" Time="1516742710939">Tue Jan 23 21:25:10 UTC 2018</LastExecutionTime> </Node> <Node Name="PRODWEST"> <Deployment Id="StandardMetadata.jc99dt9rjc99dt9s" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jc99dt9rjc99dt9s"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODWEST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="22" Minute="14" Second="23" ZoneOffsetMillis="0" Time="1515708863686">Thu Jan 11 22:14:23 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5015EDU_0921121012507.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="22" Minute="14" Second="23" ZoneOffsetMillis="0" Time="1515708863686">Thu Jan 11 22:14:23 UTC 2018</LastExecutionTime> </Node> <Node Name="PRODEAST"> <Deployment Id="StandardMetadata.jc99dt9rjc99dt9y" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jc99dt9rjc99dt9y"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODEAST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="22" Minute="34" Second="1" ZoneOffsetMillis="0" Time="1515710041962">Thu Jan 11 22:34:01 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5015EDU_0921121012507.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="22" Minute="34" Second="1" ZoneOffsetMillis="0" Time="1515710041962">Thu Jan 11 22:34:01 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM en_US jc99dso6 2017-11-14 07:07:14 true true internet/product_SKU LSE N en 09/21/19 12:10 PM 1515711332202 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:14 1665015edu-ligation-transformation-module 1665015EDU Ligation And Transformation Module Ligation And Transformation Module Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5015EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5015EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5015EDU_feature.jpg Ligation and Transformation Module #166-5015EDU RepEx Ligation and Transformation Module #166-5015EDU Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only 1665015, 1665015EDU, ligation, and, transformation, module, fifth, cloning, sequencing, explorer, series, ligase, buffer, proofreading, dna, polymerase, vector, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 <p>The fifth module in Cloning and Sequencing Explorer Series, the Ligation and Transmformation Module allows the ligation of PCR products into the pJET1.2 plasmid vector for transformation of competent bacteria. The kit includes T4 ligase, cloning vector, proofreading polymerase, buffers, C-Growth medium, transformation reagents, microcentrifuge tubes, and curriculum. Materials are sufficient for 12 student workstations. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-5015EDU_0921121012507_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-5015EDU_0921121012507_en.html 1139 <b>Kit contains sufficient materials for 12 student workstations (2&ndash;4 students per workstation)</b> &nbsp; 1 0 2691 T4 DNA ligase, 10 &micro;l 1 1 0 2692 Ligation reaction buffer, 2x, 100 &micro;l 1 1 0 2693 Proofreading DNA polymerase, 10 &micro;l 1 1 0 2694 pJET1.2 blunted cloning vector, 10 &micro;l 1 1 0 1380 BgIII restriction enzyme, 55 &micro;l 1 1 0 2695 Restriction enzyme buffer, 10x, 100 &micro;l 1 1 0 1397 C-Growth medium, 30 ml 1 1 0 2696 Transformation reagent A, 5 ml 1 1 0 2697 Transformation reagent B, 5 ml 1 1 0 2698 IPTG ,1 M, 0.1 ml 1 1 0 2699 Sterile water, 1 ml 1 1 0 2421 Colored microcentrifuge tubes, 2.0 ml 120 1 0 2428 Microcentrifuge tubes, 1.5 ml 30 1 0 2398 Curriculum, including teacher's guide, student manual, and graphic quick guide 1 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665015EDU&CartQuote=QT&quantity= 1711 false true false $406.25 true %24325.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665015EDU&AddToDefaultWishList=true&quantity= true false true 41106600 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665015EDU&quantity= 1665015EDU
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Ligation Module Reagent Refill

1665016EDU
Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only

1665016EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5016EDU_0921121013278.xml 2 1665016EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcrucx0hjcrucx0i" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcrucx0hjcrucx0i"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="23" DayOfWeek="2" Year="2018" Hour="21" Minute="25" Second="10" ZoneOffsetMillis="0" Time="1516742710939">Tue Jan 23 21:25:10 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5016EDU_0921121013278.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="23" DayOfWeek="2" Year="2018" Hour="21" Minute="25" Second="10" ZoneOffsetMillis="0" Time="1516742710939">Tue Jan 23 21:25:10 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM en_US jcrucx04 2017-11-14 07:07:15 true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:15 1665016edu-ligation-module-reagent-refill 1665016EDU Ligation Module Reagent Refill Ligation Module Reagent Refill Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5016EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5016EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5016EDU_feature.jpg RepEx 166-5016EDU Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665016EDU&CartQuote=QT&quantity= 1711 false true false $185.00 true %24148.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665016EDU&AddToDefaultWishList=true&quantity= true false true 41106600 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665016EDU&quantity= 1665016EDU
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Transformation Module Reagent Refill

1665017EDU
Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only

1665017EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5017EDU_0921121013316.xml 3 1665017EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5017EDU_0921121013316.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM en_US jcrucwzx 2017-11-14 07:07:15 true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:15 1665017edu-transformation-module-reagent-refill 1665017EDU Transformation Module Reagent Refill Transformation Module Reagent Refill Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5017EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5017EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5017EDU_feature.jpg RepEx 166-5017EDU Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665017EDU&CartQuote=QT&quantity= 1711 false true false $193.75 true %24155.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665017EDU&AddToDefaultWishList=true&quantity= true false true 41106600 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665017EDU&quantity= 1665017EDU
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Bgl II Reagent Refill

1665018EDU
Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only

1665018EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5018EDU_0921121013297.xml 4 1665018EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcrucx0hjcrucx0i" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcrucx0hjcrucx0i"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="23" DayOfWeek="2" Year="2018" Hour="21" Minute="25" Second="10" ZoneOffsetMillis="0" Time="1516742710939">Tue Jan 23 21:25:10 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5018EDU_0921121013297.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="23" DayOfWeek="2" Year="2018" Hour="21" Minute="25" Second="10" ZoneOffsetMillis="0" Time="1516742710939">Tue Jan 23 21:25:10 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM en_US jcrucx0e 2017-11-14 07:07:15 true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:15 1665018edu-bgl-ii-reagent-refill 1665018EDU Bgl II Reagent Refill Bgl II Reagent Refill Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5018EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5018EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5018EDU_feature.jpg RepEx 166-5018EDU Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665018EDU&CartQuote=QT&quantity= 1711 false true false $57.50 true %2446.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665018EDU&AddToDefaultWishList=true&quantity= true false true 12352200 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665018EDU&quantity= 1665018EDU
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1665019 Instruction Manual, Ligation and Transformation Module, Biotechnology Explorer, Rev A Click to download [ Add to Cart (Free) ]

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