iTaq™ Universal SYBR® Green Supermix

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iTaq Universal SYBR Green Supermix

Overview

Superior Gene Expression Analysis
Superior Gene Expression Analysis

iTaq™ universal SYBR® Green supermix provides reliable gene expression data. The human β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using a CFX96™ real-time PCR detection system. iTaq™ universal SYBR® Green supermix produced greater than 90% efficiency over 6 orders of linear dynamic range. Standard curve R2 = 0.999, β-actin efficiency = 92.6%, slope =
–3.51. RFU, relative fluorescence units.

Sensitive and Efficient Amplification of Genomic DNA
Superior Gene Expression Analysis

iTaq™ universal SYBR® Green supermix allows robust amplification of genomic DNA. The human GAPDH gene was amplified from human genomic DNA (50 ng–5 pg) using a CFX96™ real-time PCR detection system. iTaq™ universal SYBR® Green supermix produced a GAPDH efficiency of 108.1% over several orders of linear dynamic range. Standard curve R2 = 0.993, slope = –3.14. RFU, relative fluorescence units.

Consistent Results across Real-Time Instrument Platforms
Consistent Results Across Platforms

 

Sensitive and efficient gene expression analysis on any real-time PCR instrument. The β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using iTaq™ universal SYBR® Green supermix on several real-time instruments. A, Bio-Rad® CFX96™ real-time PCR detection system; B, Bio-Rad® iQ™5 real-time PCR detection system; C, ABI 7900 real-time PCR system; D, ABI 7500 fast real-time PCR system; E, ABI ViiA7 real-time PCR system; F, ABI StepOnePlus real-time PCR system. This supermix showed excellent β-actin efficiencies, R2, and slopes on all platforms (>90% efficiency, R2 > 0.990, slope between –3.20 and –3.58). RFU, relative fluorescence units.

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iTaq Universal SYBR Green Supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, SYBR® Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and fluorescein).

iTaq Universal SYBR Green Supermix lets you:

iTaq Universal SYBR Green Supermix contains:

  • Advanced buffer formulation with SYBR Green I for optimal qPCR performance
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
  • Blend of passive reference dyes for universal real-time instrument compatibility

Instrument Compatibility

iTaq Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection

Storage at –20°C Guaranteed for 12 months protected from light in a constant temperature freezer
Repeated freeze/thaw cycles Up to 10 times
Storage at 4°C Guaranteed for 3 months protected from light
Polymerase iTaq DNA polymerase
Hot-start mechanism Antibody mediated
Hot-start activation 95°C, 30 sec
Supermix concentration 2x
Thermal cycling protocol Standard and fast
Instrument compatibility Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems
Template compatibility cDNA, genomic DNA, plasmid DNA
Detection method SYBR® Green
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iTaq™ Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725120
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

1725120 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5120_1330471927.xml 1725120 02/28/12 03:11 PM en_US true internet/product_SKU 2018-05-31 22:17:28 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5120_1330471927.xml /default/main/bio-rad/WORKAREA/default jayharvz 2017-11-14 07:07:36 true jayharvz templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5120_1330471927.xml LSR N /default/main/bio-rad en 02/28/22 03:26 PM 1520306493287 2018-05-31 22:17:28 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5120_1330471927.xml 1725120-itaq-universal-sybr-green-supermix-200-x-20-ul-rxns-2-ml-2-x-1-ml 1 1725120 iTaq Universal SYBR Green Supermix iTaq™ Universal SYBR<sup>®</sup> Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml) 200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes 200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5120_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5120_view.jpg Karen Moss 172-5120, 1725120, itaq, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, dna, hot-start, hot, start, polymerase, mgcl2, fluorescein, rox 02/28/12 03:11 PM 02/28/22 03:26 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).</p> <ul> <li>Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument</li> <li>Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)</li> <li>Amplify genomic DNA and difficult amplicons</li> <li>Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal SYBR Green Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td valign="top"><strong>Supermix Volume, ml</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">200</td> <td valign="top">2 x 1</td> <td valign="top"><span>1725120</span></td> </tr> <tr> <td valign="top">500</td> <td valign="top">5 x 1</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5121">1725121</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">1,000</td> <td valign="top">10 x 1</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5122">1725122</a></td> </tr> <tr> <td valign="top">2,500</td> <td valign="top">5 x 5</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5124">1725124</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">5,000</td> <td valign="top">10 x 5</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5125">1725125</a></td> </tr> </tbody> </table> <p>See the <a href="/en-us/product/itaq-universal-supermixes/itaq-universal-sybr-green-supermix">iTaq Universal SYBR&nbsp;Green Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iTaq&trade; Universal SYBR<sup>&reg;</sup> Green Supermix <a href="/webroot/web/pdf/lsr/literature/10041157.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 172-5120_desc.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-5120_desc.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725120&CartQuote=QT&quantity= 1458 false true false $144.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725120&AddToDefaultWishList=true&quantity= true false true 31161500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725120&quantity= 1725120
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iTaq™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725121
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes

1725121 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5121_1330472689.xml 1725121 02/28/12 03:32 PM en_US true internet/product_SKU 2018-05-31 22:17:27 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5121_1330472689.xml /default/main/bio-rad/WORKAREA/default jayharva 2017-11-14 07:07:36 true jayharva templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5121_1330472689.xml LSR N /default/main/bio-rad en 02/28/22 03:37 PM 1520306493344 2018-05-31 22:17:27 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5121_1330472689.xml 1725121-itaq-universal-sybr-green-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml 1 1725121 iTaq Universal SYBR Green Supermix iTaq™ Universal SYBR<sup>®</sup> Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml) 500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes 500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq; DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5121_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5121_view.jpg Karen Moss 500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq&trade; DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes 172-5121, 1725121, itaq, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, dna, hot-start, hot, start, polymerase, mgcl2, fluorescein, rox 02/28/12 03:32 PM 02/28/22 03:37 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).</p> <ul> <li>Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument</li> <li>Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)</li> <li>Amplify genomic DNA and difficult amplicons</li> <li>Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal SYBR Green Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td valign="top"><strong>Supermix Volume, ml</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">200</td> <td valign="top">2 x 1</td> <td valign="top"><a href="/en-us/sku/1725120-itaq-universal-sybr-green-supermix"><span>1725120</span></a></td> </tr> <tr> <td valign="top">500</td> <td valign="top">5 x 1</td> <td valign="top"><span>1725121</span></td> </tr> <tr class="pd_colorbackground"> <td valign="top">1,000</td> <td valign="top">10 x 1</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5122">1725122</a></td> </tr> <tr> <td valign="top">2,500</td> <td valign="top">5 x 5</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5124">1725124</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">5,000</td> <td valign="top">10 x 5</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5125">1725125</a></td> </tr> </tbody> </table> <p>See the <a href="/en-us/product/itaq-universal-supermixes/itaq-universal-sybr-green-supermix">iTaq Universal SYBR&nbsp;Green Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iTaq Universal SYBR&nbsp;Green Supermix <a href="/webroot/web/pdf/lsr/literature/10041157.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs 172-5121_desc.html /webroot/web/html/lsr/products/SKUs/172-5121_desc.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725121&CartQuote=QT&quantity= 1458 false true false $304.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725121&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725121&quantity= 1725121
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iTaq™ Universal SYBR® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725122
1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

1725122 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5122_1330473017.xml 1725122 02/28/12 03:46 PM en_US true internet/product_SKU 2018-05-31 22:17:27 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5122_1330473017.xml /default/main/bio-rad/WORKAREA/default jayharv5 2017-11-14 07:07:36 true jayharv5 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5122_1330473017.xml LSR N /default/main/bio-rad en 02/28/22 03:48 PM 1520306493391 2018-05-31 22:17:27 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5122_1330473017.xml 1725122-itaq-universal-sybr-green-supermix-1000-x-20-ul-rxns-10-ml-10-x-1-ml 1 1725122 iTaq Universal SYBR Green Supermix iTaq™ Universal SYBR<sup>®</sup> Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml) 1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes 1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5122_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5122_view.jpg Karen Moss 172-5122, 1725122, itaq, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, dna, hot-start, hot, start, polymerase, mgcl2, fluorescein, rox 02/28/12 03:46 PM 02/28/22 03:48 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).</p> <ul> <li>Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument</li> <li>Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)</li> <li>Amplify genomic DNA and difficult amplicons</li> <li>Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal SYBR Green Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td valign="top"><strong>Supermix Volume, ml</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">200</td> <td valign="top">2 x 1</td> <td valign="top"><a href="/en-us/sku/1725120-itaq-universal-sybr-green-supermix">1725120</a></td> </tr> <tr> <td valign="top">500</td> <td valign="top">5 x 1</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5121">1725121</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">1,000</td> <td valign="top">10 x 1</td> <td valign="top">1725122</td> </tr> <tr> <td valign="top">2,500</td> <td valign="top">5 x 5</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5124">1725124</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">5,000</td> <td valign="top">10 x 5</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5125">1725125</a></td> </tr> </tbody> </table> <p>See the <a href="/en-us/product/itaq-universal-supermixes/itaq-universal-sybr-green-supermix">iTaq Universal SYBR&nbsp;Green Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iTaq Universal SYBR&nbsp;Green Supermix <a href="/webroot/web/pdf/lsr/literature/10041157.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 172-5122_desc.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-5122_desc.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725122&CartQuote=QT&quantity= 1458 false true false $582.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725122&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725122&quantity= 1725122
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iTaq™ Universal SYBR® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725124
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

1725124 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5124_1330474548.xml 1725124 02/28/12 03:57 PM en_US true internet/product_SKU 2018-05-31 22:17:28 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5124_1330474548.xml /default/main/bio-rad/WORKAREA/default jayharvt 2017-11-14 07:07:36 true jayharvt templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5124_1330474548.xml LSR N /default/main/bio-rad en 02/28/22 04:01 PM 1520306493518 2018-05-31 22:17:28 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5124_1330474548.xml 1725124-itaq-universal-sybr-green-supermix-2500-x-20-ul-rxns-25-ml-5-x-5-ml 1 1725124 iTaq Universal SYBR Green Supermix iTaq™ Universal SYBR<sup>®</sup> Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml) 2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes 2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5124_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5124_view.jpg Karen Moss 172-5124, 1725124, itaq, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, dna, hot-start, hot, start, polymerase, mgcl2, fluorescein, rox 02/28/12 03:57 PM 02/28/22 04:01 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).</p> <ul> <li>Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument</li> <li>Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)</li> <li>Amplify genomic DNA and difficult amplicons</li> <li>Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal SYBR Green Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td valign="top"><strong>Supermix Volume, ml</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">200</td> <td valign="top">2 x 1</td> <td valign="top"><a href="/en-us/sku/1725120-itaq-universal-sybr-green-supermix">1725120</a></td> </tr> <tr> <td valign="top">500</td> <td valign="top">5 x 1</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5121">1725121</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">1,000</td> <td valign="top">10 x 1</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5122">1725122</a></td> </tr> <tr> <td valign="top">2,500</td> <td valign="top">5 x 5</td> <td valign="top">1725124</td> </tr> <tr class="pd_colorbackground"> <td valign="top">5,000</td> <td valign="top">10 x 5</td> <td valign="top"><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5125">1725125</a></td> </tr> </tbody> </table> <p>See the <a href="/en-us/product/itaq-universal-supermixes/itaq-universal-sybr-green-supermix">iTaq&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iTaq&trade; Universal SYBR<sup>&reg;</sup> Green Supermix <a href="/webroot/web/pdf/lsr/literature/10041157.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 172-5174_desc.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-5174_desc.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725124&CartQuote=QT&quantity= 1458 false true false $1,344.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725124&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725124&quantity= 1725124
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iTaq™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725125
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

1725125 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5125_1330474891.xml 1725125 02/28/12 04:16 PM en_US true internet/product_SKU 2018-05-31 22:17:28 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5125_1330474891.xml /default/main/bio-rad/WORKAREA/default jayharvf 2017-11-14 07:07:36 true jayharvf templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5125_1330474891.xml LSR N /default/main/bio-rad en 02/28/22 04:18 PM 1520306493573 2018-05-31 22:17:28 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5125_1330474891.xml 1725125-itaq-universal-sybr-green-supermix-5000-x-20-ul-rxns-50-ml-10-x-5-ml 1 1725125 iTaq Universal SYBR Green Supermix iTaq™ Universal SYBR<sup>®</sup> Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml) 5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes 5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5125_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5125_view.jpg Karen Moss 172-5125, 1725125, itaq, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, dna, hot-start, hot, start, polymerase, mgcl2, fluorescein, rox 02/28/12 04:16 PM 02/28/22 04:18 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).</p> <ul> <li>Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument</li> <li>Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)</li> <li>Amplify genomic DNA and difficult amplicons</li> <li>Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal SYBR Green Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td valign="top"><strong>Supermix Volume, ml</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">200</td> <td valign="top">2 x 1</td> <td valign="top"><a href="/en-us/sku/1725120-itaq-universal-sybr-green-supermix"><span style="font-size: 11px;">1725120</span></a></td> </tr> <tr> <td valign="top">500</td> <td valign="top">5 x 1</td> <td valign="top"><a style="font-size: 11px;" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5121">1725121</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">1,000</td> <td valign="top">10 x 1</td> <td valign="top"><a style="font-size: 11px;" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5122">1725122</a></td> </tr> <tr> <td valign="top">2,500</td> <td valign="top">5 x 5</td> <td valign="top"><a style="font-size: 11px;" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5124">1725124</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">5,000</td> <td valign="top">10 x 5</td> <td valign="top">1725125</td> </tr> </tbody> </table> <p>See the <a href="/en-us/product/itaq-universal-supermixes/itaq-universal-sybr-green-supermix">iTaq Universal SYBR Green Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iTaq&trade; Universal SYBR<sup>&reg;</sup> Green Supermix <a href="/webroot/web/pdf/lsr/literature/10041157.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 172-5125_desc.html /webroot/web/html/lsr/products/SKUs/autopopulated/172-5125_desc.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725125&CartQuote=QT&quantity= 1458 false true false $2,549.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725125&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true 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6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
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