iTaq™ Universal SYBR® Green Supermix

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Overview

Superior Gene Expression Analysis

iTaq™ Universal SYBR® Green Supermix provides reliable gene expression data.

The human β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using a CFX96™ real-time PCR detection system. iTaq Universal SYBR® Green Supermix produced greater than 90% efficiency over 6 orders of linear dynamic range. Standard curve R2 = 0.999, β-actin efficiency = 92.6%, slope =
–3.51. RFU, relative fluorescence units.



Sensitive and Efficient Amplification of Genomic DNA

iTaq™ Universal SYBR® Green Supermix allows robust amplification of genomic DNA.

The human GAPDH gene was amplified from human genomic DNA (50 ng–5 pg) using a CFX96™ real-time PCR detection system. iTaq Universal SYBR® Green Supermix produced a GAPDH efficiency of 108.1% over several orders of linear dynamic range. Standard curve R2 = 0.993, slope = –3.14. RFU, relative fluorescence units.



Consistent Results across Real-Time Instrument Platforms

iTaq™ Universal SYBR® Green Supermix delivers sensitive and efficient gene expression analysis on any real-time PCR instrument.

The β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using iTaq Universal SYBR® Green Supermix on several real-time instruments. The iTaq Universal SYBR® Green Supermix showed excellent amplification efficiency, R2, and slopes on all platforms (>90% efficiency, R2 > 0.990, slope between –3.20 and –3.58).

A, Bio-Rad® CFX96™ Real-Time PCR Detection System

B, Bio-Rad® iQ™5 Real-Time PCR

C, ABI 7900 Real-Time PCR System

D, ABI 7500 Fast Real-Time PCR System

E, ABI ViiA7 Real-Time PCR System

F, ABI StepOnePlus Real-Time PCR System



Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3D animated videos, technical manuals and more.

iTaq™ universal SYBR® Green supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, SYBR® Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and fluorescein).

iTaq™ universal SYBR® Green supermix lets you:

iTaq™ universal SYBR® Green supermix contains:

  • Advanced buffer formulation with SYBR® Green I for optimal qPCR performance
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
  • Blend of passive reference dyes for universal real-time instrument compatibility

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection

Storage at –20°C Guaranteed for 12 months protected from light in a constant temperature freezer
Repeated freeze/thaw cycles Up to 10 times
Storage at 4°C Guaranteed for 3 months protected from light
Polymerase iTaq DNA polymerase
Hot-start mechanism Antibody mediated
Hot-start activation 95°C, 30 sec
Supermix concentration 2x
Thermal cycling protocol Standard and fast
Instrument compatibility Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems
Template compatibility cDNA, genomic DNA, plasmid DNA
Detection method SYBR® Green
x

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iTaq™ Universal SYBR® Green Supermix

1725120
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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$130.00
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iTaq™ Universal SYBR® Green Supermix

1725121
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes

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$275.00
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iTaq™ Universal SYBR® Green Supermix

1725122
1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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$530.00
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iTaq™ Universal SYBR® Green Supermix

1725124
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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$1,225.00
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iTaq™ Universal SYBR® Green Supermix

1725125
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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$2,330.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
10041157 iTaq™ Universal SYBR® Green Supermix Product Insert, Rev A Click to download
6090 Amplification Reagents and Plastics Brochure, Rev E Click to download [ Add to Cart (Free) ]