iTaq™ Universal Probes Supermix

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iTaq universal probes supermix is a 2x concentrated, ready-to-use reaction master mix containing all components, except primers, probes, and template, for probe-based quantitative PCR (qPCR; simplex or duplex reaction) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, enhancers, stabilizers, and a blend of passive reference dyes (including ROX).

iTaq universal probes supermix lets you:

iTaq universal probes supermix contains:

  • Advanced buffer formulation for optimal probe-based qPCR
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
  • Blend of passive reference dyes for universal real-time instrument compatibility

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection

Storage at –20°C Guaranteed for 12 months protected from light in a constant temperature freezer
Repeated freeze/thaw cycles Up to 10 times
Storage at 4°C Guaranteed for 3 months protected from light
Polymerase iTaq DNA polymerase
Hot-start mechanism Antibody mediated
Hot-start activation 95°C, 30 sec
Supermix concentration 2x
Thermal cycling protocol Standard and fast
Instrument compatibility Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems
Template compatibility cDNA, genomic DNA, plasmid DNA
Detection method Fluorogenic probes
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iTaq™ Universal Probes Supermix

1725130
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

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$130.00
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iTaq™ Universal Probes Supermix

1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes

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$275.00
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iTaq™ Universal Probes Supermix

1725132
1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

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$530.00
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iTaq™ Universal Probes Supermix

1725134
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

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$1,225.00
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iTaq™ Universal Probes Supermix

1725135
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

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$2,330.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
10041156 iTaq™ Universal Probes Supermix Product Insert, Rev A Click to download
6090 Amplification Reagents and Plastics Brochure, Rev E Click to download [ Add to Cart (Free) ]