iScript™ Select cDNA Synthesis Kit

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iScript Select cDNA Synthesis Kit

Overview

The iScript™ Select Kit Demonstrates Superior Fidelity
The iScript Select Kit Demonstrates Superior Fidelity

The iScript Select Kit demonstrates superior fidelity when compared to several other commonly used reverse transcriptases. A partial fragment of the ampicillin resistance gene was reverse transcribed using one of six different commonly used reverse transcriptases according to manufacturer’s instructions. PCR was performed using Bio-Rad’s iProof™ High-Fidelity Master Mix. Resulting amplicons were sequenced using the Illumina MiSeq platform. For each sample, 10 million bases of valid reads with three or more repeats were obtained. Fidelity of each reverse transcriptase was determined by taking the inverse of the error rate. Mean accuracy of four replicates is shown. Error bars are standard errors of four replicates.

Long cDNA Synthesis

The iScript Select cDNA synthesis kit facilitates synthesis of cDNA longer than 6 kb. First-strand cDNA was produced from 1 µg total RNA using the iScript Select cDNA synthesis kit and the provided oligo(dT) primer mix. A 2 µl aliquot of the 20 µl cDNA synthesis reaction was subjected to 35 PCR cycles using a proofreading high-fidelity polymerase and human adenomatous polyposis coli (APC) primer sets. From a 50 µl PCR reaction, 10 µl was analyzed on a 1% agarose gel. Lane 1, 1 kb ladder; lanes 2 and 3, 6.4 kb APC PCR product; lanes 4 and 5, 8.2 kb APC PCR product.

Broad Dynamic Range

The iScript™ Select cDNA synthesis kit performs reliably over six orders of magnitude using any first-strand priming approach. Human total RNA from 1 pg to 1 µg was reverse transcribed using the iScript Select cDNA synthesis kit and the first-strand primers indicated above. The resulting cDNA was amplified in triplicate using iQ™ SYBR® Green supermix with β-actin primers and detected using the iCycler iQ® real-time detection system, RFU, relative fluorescence units.

RNaseA Inhibition
 

 

 

RNase levels in different RNA samples. RNaseA, water, RT buffer (used to dilute RNaseA), spleen RNA (S RNA-1 and S RNA-2), and pancreas RNA (P RNA-1 and P RNA-2) from two different preparations were assayed for RNaseA activity using a commercially available kit. RNaseA (several fold higher than normally found in contaminated RNA preparations) was used as control to demonstrate varying levels of RNaseA activity in sample preparations.

 

RNase inhibition by the iScript™ cDNA synthesis kit. RNaseA, water, RT buffer (used to dilute RNaseA), and RNase + iScript cDNA kit were assayed for RNaseA activity using a commercially available kit. RNaseA (several fold higher than normally found in contaminated RNA preparations) was used as control to assess the effect of inhibition by the iScript cDNA kit.

 

Functional RT-qPCR assay demonstrating RNaseA inhibition by the iScript cDNA synthesis kit. 18S rRNA was amplified using iQ™ SYBR® Green supermix. Reverse transcription (RT) performed with iScript kit (); RT performed with spiked RNaseA (); RT performed with RNaseA but no RNase inhibitor (■). Two RNA inputs (1,000 and 0.1 pg) were reverse transcribed for all three groups. The cDNA synthesis reaction with RNase but no RNase inhibitor showed significant threshold cycle (CT) delay (3–5), whereas the iScript kit demonstrated powerful inhibition of RNaseA. RFU, relative fluorescence units.

Which iScript™ Kit is right for me?
 

Most
Convenient

Integrated
DNA
Removal

Maximum
Input Use

Flexible Priming,
High-Fidelity

Value
Solution

Product iScript Reverse Transcription Supermix for RT-qPCR iScript gDNA
Clear cDNA
Synthesis Kit
iScript Advanced cDNA Synthesis Kit for RT-qPCR iScript Select cDNA
Synthesis Kit
iScript cDNA
Synthesis Kit
Applications
  • Gene expression
  • Gene expression
  • Gene expression
  • Cloning
  • Sequencing and NGS
  • Gene expression
  • Gene expression
Total Input RNA 1 µg–1 pg 1 µg–1 pg 7.5 µg–100 fg 1 µg–1 pg 1 µg–100 fg
Format 1 tube 3 tubes 2 tubes 5 tubes 2 tubes
Kit Contents
  • 5x iScript RT Supermix
  • No-RT control supermix
  • 5x iScript RT Supermix
  • No-RT control supermix
  • DNase
  • DNase buffer
  • iScript Reverse Transcriptase
  • 5x iScript Advanced Reaction Mix
  • iScript Reverse Transcriptase
  • 5x iScript Reaction Mix
  • 3 priming options
  • iScript Reverse Transcriptase
  • 5x iScript Reaction Mix
Time to Produce cDNA 26 min 36 min 21 min 15–65 min 26 min
RNase H+ Activity High High High High High
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The iScript Select cDNA Synthesis Kit is a high-fidelity, sensitive reverse transcription system that provides ultimate flexibility. Unlike our other iScript cDNA Synthesis products, the reaction components for this kit are packaged in individual tubes, enabling flexible priming options and customization of reaction setup for specific application needs.

Key Features and Benefits

  • Achieve high-fidelity — unique reverse transcription kit minimizes introduction of base pair errors
  • Synthesize long cDNAs — efficiently synthesize cDNA greater than 8 kb
  • Save time and reduce errors during setup — less hands on time and less pipetting steps compared to protocols for other high-fidelity reverse transcriptase kits
  • Detect low-level target genes and conserve RNA — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription


High-Fidelity

iScript Select cDNA Synthesis Kit is designed for applications requiring high-fidelity, such as cloning and NGS. This kit introduces fewer base pair errors than other commonly used reverse transcription kits.

Ideal RT for Many Applications

The combination of iScript Select Kit performance and its packaging of various priming options in individual tubes enables this reagent to perform optimally across many different applications.

A versatile reverse transcription kit serves all of your RT-PCR needs

  • Cloning

    Cloning

  • RT-qPCR

    RT-qPCR

  • NGS

    NGS

  • Sequencing

    Sequencing

Related Products

Choose the best cDNA synthesis kit for your application, with formulations optimized for ease, yield, flexibility, or gDNA clearance.

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature
freezer (multiple freeze thaws not recommended)
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iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns

1708896
25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

0 1708896 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8896_0921120538320.xml 1708896 09/21/09 12:05 PM en_US true internet/product_SKU 2018-05-07 23:22:02 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8896_0921120538320.xml /default/main/bio-rad/WORKAREA/default jcrucyrn 2017-11-14 07:07:38 true jcrucyrn templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8896_0921120538320.xml LSR N /default/main/bio-rad en 09/21/19 12:05 PM 1519715010582 2018-05-07 23:22:02 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8896_0921120538320.xml 1708896-iscript-select-cdna-synthesis-kit-25-x-20-ul-rxns 1 1708896 iScript Select cDNA Synthesis Kit iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns 25 x 20 &#181;l reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water 25 x 20 &#181;l reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water 25 x 20 &#181;l reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8896_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8896_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8896_feature.jpg RepEx 170-8896 25 x 20 &#181;l reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water 170-8896, 1708896, iscript, select, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection, rt-qpcr 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Use the iScript Select cDNA Synthesis Kit for flexible priming strategies. Components are provided in separate tubes, allowing you to change priming strategies with only one kit.</p> <ul> <li>High-fidelity kit minimizes introduction of base pair errors</li> <li>Oligo(dT), random primer, and gene-specific primer enhancer solution in separate tubes</li> <li>Quality controlled for reliable synthesis of cDNA &mdash; synthesize long cDNAs&nbsp;&gt; 8 kb</li> <li>Broad linear dynamic range of total input RNA (1 &micro;g&ndash;1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase</li> <li>25 x 20 &micro;l reactions</li> <li>Potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription</li> </ul> <h2 class="descHeader">Packaging Options</h2> <ul> <li>25 x 20 &micro;l reactions (1708896)</li> <li>100 x 20 &micro;l reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=170-8897">1708897</a>)</li> <li>500 x 20 &micro;l reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=170-8897BUN">1708897BUN</a>)</li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR</a>: 2-tube format with RT capacity of input RNA up to 7.5 &micro;g, generates cDNA in&nbsp;21 min</li> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript&trade; Reverse Transcription Supermix for RT-qPCR</a>: 1-tube format for total input 1 &micro;g&ndash;1 pg RNA, plus liquid at &ndash;20&deg;C, eliminating freeze/thaw cycles</li> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-cdna-synthesis-kit">iScript&trade; cDNA Synthesis Kit</a>: 2-tube format allows for up to 15 &micro;l of RNA sample in a 20 &micro;l reaction, in a 26 min protocol</li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn about the <a href="/en-us/product/reverse-transcription-reagents/iscript-select-cdna-synthesis-kit?tab=Description">key features and specifications of the iScript Select cDNA Synthesis Kit</a>.</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 170-8896_0921120538320_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//170-8896_0921120538320_en.html 1725120 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5120_1330471927.xml HSP9621 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/HSP-9621_0921120534751.xml MSC1001 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/MSC-1001_1297906204.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708896&CartQuote=QT&quantity= false true false $155.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708896&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708896&quantity= 1708896
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iScript™ Select cDNA Synthesis Kit, 100 x 20 µl rxns

1708897
100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

1 1708897 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8897_0921120538371.xml 1708897 09/21/09 12:05 PM en_US true internet/product_SKU 2018-05-07 23:22:02 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8897_0921120538371.xml /default/main/bio-rad/WORKAREA/default jcrucyrw 2017-11-14 07:07:38 true jcrucyrw templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8897_0921120538371.xml LSR N /default/main/bio-rad en 09/21/19 12:05 PM 1519715010629 2018-05-07 23:22:02 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8897_0921120538371.xml 1708897-iscript-select-cdna-synthesis-kit-100-x-20-ul-rxns 1 1708897 iScript Select cDNA Synthesis Kit iScript™ Select cDNA Synthesis Kit, 100 x 20 µl rxns 100 x 20 &#181;l reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water 100 x 20 &#181;l reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water 100 x 20 &#181;l reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8897_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8897_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8897_feature.jpg RepEx 170-8897 100 x 20 &#181;l reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water 170-8897, 1708897, iscript, select, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection, rt-qpcr 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Use the iScript Select cDNA Synthesis Kit for flexible priming strategies. Components are provided in separate tubes, allowing you to change priming strategies with only one kit.</p> <ul> <li>High-fidelity kit minimizes introduction of base pair errors</li> <li>Oligo(dT), random primer, and gene-specific primer enhancer solution in separate tubes</li> <li>Quality controlled for reliable synthesis of cDNA &mdash; synthesize long cDNAs&nbsp;&gt; 8 kb</li> <li>Broad linear dynamic range of total input RNA (1 &micro;g&ndash;1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase</li> <li>100 x 20 &micro;l reactions</li> <li>Potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription</li> </ul> <h2 class="descHeader">Packaging Options</h2> <ul> <li>25 x 20 &micro;l reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=170-8896">1708896</a>)</li> <li>100 x 20 &micro;l reactions (1708897)</li> <li>500 x 20 &micro;l reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=170-8897BUN">1708897BUN</a>)</li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR</a>: 2-tube format with RT capacity of input RNA up to 7.5 &micro;g, generates cDNA in&nbsp;21 min</li> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript&trade; Reverse Transcription Supermix for RT-qPCR</a>: 1-tube format for total input 1 &micro;g&ndash;1 pg RNA, plus liquid at &ndash;20&deg;C, eliminating freeze/thaw cycles</li> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-cdna-synthesis-kit">iScript&trade; cDNA Synthesis Kit</a>: 2-tube format allows for up to 15 &micro;l of RNA sample in a 20 &micro;l reaction, in a&nbsp;26 min protocol</li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn about the <a href="/en-us/product/reverse-transcription-reagents/iscript-select-cdna-synthesis-kit?tab=Description">key features and specifications of the iScript Select cDNA Synthesis Kit</a>.</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 170-8897_0921120538371_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//170-8897_0921120538371_en.html 1725120 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5120_1330471927.xml HSP9621 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/HSP-9621_0921120534751.xml MSC1001 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/MSC-1001_1297906204.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708897&CartQuote=QT&quantity= false true false $479.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708897&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708897&quantity= 1708897
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iScript™ Select cDNA Synthesis Kit, 500 x 20 µl rxns

1708897BUN
500 x 20 µl reactions, includes 5x reaction mix, iScript Reverse Transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

1708897BUN /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8897BUN_1318357960.xml 1708897BUN 10/11/11 11:08 AM en_US true internet/product_SKU 2018-05-07 23:22:02 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8897BUN_1318357960.xml /default/main/bio-rad/WORKAREA/default jcrucyqn 2017-11-14 07:07:36 true jcrucyqn templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8897BUN_1318357960.xml LSR N /default/main/bio-rad en 10/11/21 11:08 AM 1519714504111 2018-05-07 23:22:02 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8897BUN_1318357960.xml 1708897bun-iscript-select-cdna-synthesis-kit-500-x-20-ul-rxns 1 1708897BUN iScript Select cDNA Synthesis Kit (500 reactions) iScript™ Select cDNA Synthesis Kit, 500 x 20 µl rxns 500 x 20 &micro;l reactions, includes 5x reaction mix, iScript Reverse Transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water 500 x 20 &micro;l reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8897BUN_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8897BUN_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8897BUN_feature.jpg Karen Moss iScript&trade; Select cDNA Synthesis Kit (500 reactions) 500 x 20 &micro;l reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water 170-8897BUN, 1708897BUN, iscript, select, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection, rt-qpcr 10/11/11 11:08 AM 10/11/21 11:08 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the iScript Select cDNA Synthesis Kit for flexible priming strategies. Components are provided in separate tubes, allowing you to change priming strategies with only one kit.</p> <ul> <li>High-fidelity kit minimizes introduction of base pair errors</li> <li>Oligo(dT), random primer, and gene-specific primer enhancer solution in separate tubes</li> <li>Quality controlled for reliable synthesis of cDNA &mdash; synthesize long cDNAs&nbsp;&gt; 8 kb</li> <li>Broad linear dynamic range of total input RNA (1 &micro;g&ndash;1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase</li> <li>500 x 20 &micro;l reactions</li> <li>Potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription</li> </ul> <h2 class="descHeader">Packaging Options</h2> <ul> <li>25 x 20 &micro;l reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=170-8896">1708896</a>)</li> <li>100 x 20 &micro;l reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=170-8897">1708897</a>)</li> <li>500 x 20 &micro;l reactions (1708897BUN)</li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR</a>: 2-tube format with RT capacity of input RNA up to 7.5 &micro;g, generates cDNA in 21 min</li> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript&trade; Reverse Transcription Supermix for RT-qPCR</a>: 1-tube format for total input 1 &micro;g&ndash;1 pg RNA, plus liquid at &ndash;20&deg;C, eliminating freeze/thaw cycles</li> <li><a style="font-weight: bold;" href="/en-us/product/reverse-transcription-reagents/iscript-cdna-synthesis-kit">iScript&trade; cDNA Synthesis Kit</a>: 2-tube format allows for up to 15 &micro;l of RNA sample in a 20 &micro;l reaction, in a&nbsp;26 min protocol</li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn about the <a href="/en-us/product/reverse-transcription-reagents/iscript-select-cdna-synthesis-kit?tab=Description">key features and specifications of the iScript Select cDNA Synthesis Kit</a>.</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 170-8897BUN_desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/170-8897BUN_desc.html &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708897BUN&CartQuote=QT&quantity= false true false $2,251.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708897BUN&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708897BUN&quantity= 1708897BUN
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Number Description Options
10001023 Instruction Manual, iScript™ Select cDNA Synthesis Kit, Rev B Click to download
7075 iScript™ Select cDNA Synthesis Kit Flier Click to download
7076 iScript™ Select cDNA Synthesis Kit Has Highest Fidelity as Determined by Next-Generation Sequencing Click to download
7037 iScript High-Fidelity Cloning Applications Protocol Click to download [ Add to Cart (Free) ]
6746 iScript Reverse Transcription Kits Flier Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download [ Add to Cart (Free) ]
5211 iProof High-Fidelity DNA Polymerase Flier, Rev C Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]

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