iScript™ RT-qPCR Sample Preparation Reagent

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iScript RT-qPCR Sample Preparation Reagent

Overview

Performance

iScript RT-qPCR sample preparation reagent generates linear results over varying input cell amounts. HeLa cells were treated with each reagent (125, 25, 5, and 1 cells/µl) according to the recommended guidelines and analyzed for GAPDH expression levels using iScript cDNA synthesis kit and iQ SYBR® Green supermix on the CFX96 real-time PCR detection system. iScript RT-qPCR sample preparation reagent delivers excellent efficiency and reproducibility for the detection of GAPDH from cell extracts. GAPDH efficiency = 94.4%, R2 = 0.996. RFU, relative fluorescence units.

GAPDH Expression Levels
iScript RT-qPCR Sample Preparation Reagent

PureZOL RNA Isolation Reagent

iScript RT-qPCR sample preparation reagent generates earlier threshold cycles (CTs) compared to PureZOL RNA isolation reagent. HeLa cells were treated with each reagent (125, 25, 5, and 1 cells/µl) according to the recommended guidelines and analyzed for GAPDH expression levels using iScript cDNA synthesis kit and iQ SYBR® Green supermix on the CFX96 real-time PCR detection system. iScript RT-qPCR sample preparation reagent delivers earlier CTs and comparable efficiencies to PureZOL treatment. Average CT values for triplicate reactions are shown for each dilution point (inset tables). RFU, relative fluorescence units.

18S rRNA, β-Tubulin, and C-Myc Expression Levels
iScript RT-qPCR Sample Preparation Reagent

PureZOL RNA Isolation Reagent

iScript RT-qPCR sample preparation reagent generates earlier CTs compared to PureZOL RNA isolation reagent. HeLa cells were treated with each reagent (125 cells/µl) according to the recommended guidelines and analyzed for 18S rRNA (), β-tubulin (), and c-Myc () expression levels using iScript cDNA synthesis kit and iQ SYBR® Green supermix on the CFX96 real-time PCR detection system. iScript RT-qPCR sample preparation reagent delivers earlier CTs as compared to PureZOL treatment. Average CT values for triplicate reactions are shown for each gene target (inset tables). RFU, relative fluorescence units.

Which iScript™ Kit is right for me?
 

Most
Convenient

Integrated
DNA
Removal

Maximum
Input Use

Flexible
Priming
Options

Value
Solution

iScript Reverse TranscriptionSupermix for RT-qPCR iScript gDNA Clear
cDNA Synthesis Kit
iScript Advanced cDNA Synthesis Kit for RT-qPCR iScript Select cDNA
Synthesis Kit
iScript cDNA
Synthesis Kit
  • Gene expression
  • RNA quantification
  • Gene expression
  • RNA quantification
  • Gene expression
  • RNA quantification
  • Gene expression
  • Cloning
  • RNA quantification
  • Gene expression
  • RNA quantification
1 µg–1 pg 1 µg–1 pg 7.5 µg–100 fg 1 µg–1 pg 1 µg–100 fg
1 tube 3 tubes 2 tubes 5 tubes 2 tubes
  • 5x iScript RT Supermix
  • No-RT control supermix
  • 5x iScript RT Supermix
  • No-RT control supermix
  • DNase
  • DNase buffer
  • iScript Reverse Transcriptase
  • 5x iScript Advanced Reaction Mix
  • iScript Reverse Transcriptase
  • 5x iScript Reaction Mix
  • 3 priming options
  • iScript Reverse Transcriptase
  • 5x iScript Reaction Mix
26 min 36 min 21 min 40–90 min 26 min
High High High High High
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iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without traditional RNA sample preparation.

Key Features and Benefits

  • Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis and RNA stabilization in 5–10 min
  • Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
  • Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
  • Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
  • Use with different cell types — compatible with both suspension and adherent cell cultures
Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
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iScript™ RT-qPCR Sample Preparation Reagent

1708898
100 reactions, 10 ml, contains RNA stabilizers

0 1708898 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8898_0921120552814.xml 1708898 /default/main/bio-rad/WORKAREA/default 09/21/09 12:05 PM en_US jdl0szan 2017-11-14 07:07:36 true true internet/product_SKU jdl0szan templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8898_0921120552814.xml LSR 2018-02-23 02:29:04 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-23 02:29:04 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8898_0921120552814.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8898_0921120552814.xml 1708898-iscript-rt-qpcr-sample-preparation-reagent 1 1708898 Script RT-qPCR Sample Preparation Reagent iScript&trade; RT-qPCR Sample Preparation Reagent 100 reactions, 10 ml, contains RNA stabilizers 100 reactions, 10 ml, contains RNase inhibitors and RNA stabilizers 100 reactions, 10 ml, contains RNA stabilizers /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8898_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8898_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8898_feature.jpg RepEx 170-8898 100 reactions, 10 ml, contains100 reactions, 10 ml, contains RNA stabilizers 1708898, iscript, rt-qpcr, sample, preparation, reagent, cell, lysis, rna, extraction, purification, stabilizer 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p><strong> Packaging Options</strong></p> <ul> <li><strong>Catalog #170-8898</strong>, 100 reactions, 10 ml, contains RNase inhibitors and RNA stabilizers</li> <li><strong>Catalog #<a href="/evportal/destination/commerce/sku_detail?productID=170-8899">170-8899</a></strong>, 500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers</li> </ul> <p><strong> Description</strong></p> <p>iScript&trade; RT-qPCR sample preparation reagent provides a simple and rapid procedure (&lt;10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without traditional RNA sample preparation.</p> <p><strong> Key Features and Benefits</strong></p> <ul> <li><strong>Have ease-of-use and simplicity</strong> &mdash; one-buffer formulation contains all of the necessary components to perform cell lysis and RNA stabilization in 5&ndash;10 min</li> <li><strong>Benefit from superior compatibility with qPCR directly from cells</strong> &mdash; sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents</li> <li><strong>Maximize data from very few cells</strong> &mdash; reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells</li> <li><strong>Extend isolated RNA shelf-life</strong> &mdash; isolated RNA is stable for up to 6 months when frozen</li> <li><strong>Use with different cell types</strong> &mdash; compatible with both suspension and adherent cell cultures</li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 170-8898_0921120552814_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//170-8898_0921120552814_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708898&CartQuote=QT&quantity= false true false $160.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708898&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708898&quantity= 1708898
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iScript™ RT-qPCR Sample Preparation Reagent

1708899
500 reactions, 5 x 10 ml, contains RNA stabilizers

1 1708899 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8899_0921120552832.xml 1708899 /default/main/bio-rad/WORKAREA/default 09/21/09 12:05 PM en_US jdl0szak 2017-11-14 07:07:36 true true internet/product_SKU jdl0szak templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8899_0921120552832.xml LSR 2018-02-23 02:29:03 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-23 02:29:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8899_0921120552832.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8899_0921120552832.xml 1708899-iscript-rt-qpcr-sample-preparation-reagent 1 1708899 iScript RT-qPCR Sample Preparation Reagent iScript&trade; RT-qPCR Sample Preparation Reagent 500 reactions, 5 x 10 ml, contains RNA stabilizers 500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers 500 reactions, 5 x 10 ml, contains RNA stabilizers /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8899_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8899_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8899_feature.jpg RepEx 170-8899 500 reactions, 5 x 10 ml, contains RNA stabilizers cDNA, cDNA synthesis kit, RT, reverse transcription, reverse transcriptase, first strand, complimentary DNA, qPCR, RT-qPCR, real-time qPCR, SYBr Green, EvaGreen, FAM, HEX probes, multiplex, multiplexing, simplex, RNA isolation, Hela cell RNA, qPCR primers, hotstart DNA polymerase, real-time qPCR mixes, mastermix, 1-step RT-qPCR, 2-step-RT-qpcr, omniscript, superscript, maxima, go-script, Superscript II, superscript IIII, high-capacity, Maxima, primescript, qscript, quantitect, versa, retroscript, vilo, qiagen omniscript, quantitect, cells-to-ct, cellsdirect, qPCR cells, RT-qPCR sample preparation reagent, one-buffer RT formulation, qRT-PCR direct from cells, iScript reverse transcription, qPCR reagents, cellsdirect, iScript RT, RT sample prep, iScript sample prep, one-step RT-qPCR, two-step-RT-qpcr, one step RT-qPCR, two step-RT-qpcr, 1 step RT-qPCR, 2 step-RT-qpcr, taqman gene expression cells-to-ct, taqman gene expression cells to ct, taqman preamp cells-to-ct, taqman preamp cells to ct, sybr green cells-to-ct, sybr green cells to ct 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p><strong> Packaging Options</strong></p> <ul> <li><strong>Catalog #<a href="/evportal/destination/commerce/sku_detail?productID=170-8898">170-8898</a></strong>, 100 reactions, 10 ml, contains RNase inhibitors and RNA stabilizers</li> <li><strong>Catalog #170-8899</strong>, 500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers</li> </ul> <p><strong> Description</strong></p> <p>iScript&trade; RT-qPCR sample preparation reagent provides a simple and rapid procedure (&lt;10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without traditional RNA sample preparation.</p> <p><strong> Key Features and Benefits</strong></p> <ul> <li><strong>Have ease-of-use and simplicity</strong> &mdash; one-buffer formulation contains all of the necessary components to perform cell lysis and RNA stabilization in 5&ndash;10 min</li> <li><strong>Benefit from superior compatibility with qPCR directly from cells</strong> &mdash; sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents</li> <li><strong>Maximize data from very few cells</strong> &mdash; reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells</li> <li><strong>Extend isolated RNA shelf-life</strong> &mdash; isolated RNA is stable for up to 6 months when frozen</li> <li><strong>Use with different cell types</strong> &mdash; compatible with both suspension and adherent cell cultures</li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 170-8899_0921120552832_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//170-8899_0921120552832_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708899&CartQuote=QT&quantity= false true false $561.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708899&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708899&quantity= 1708899
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Number Description Options
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
10013596 Instruction Manual, iScript RT-qPCR Sample Preparation Reagent, Rev A Click to download
6763 Gene Expression Real-Time PCR Workflow Flier, Ver A Click to download [ Add to Cart (Free) ]
5924 Stem Cell Basics for Life Science Researchers Brochure, Rev A Click to download [ Add to Cart (Free) ]

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