iScript™ gDNA Clear cDNA Synthesis Kit

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iScript™ gDNA Clear cDNA Synthesis Kit for Genomic DNA-free cDNA Synthesis

Overview

Effective Removal of Genomic DNA during cDNA Synthesis

No amplification was observed from Bio-Rad iScript™ gDNA Clear cDNA Synthesis Kit (A), demonstrating complete removal of gDNA.

Effective Removal of Genomic DNA during cDNA Synthesis

1 µg of HeLa RNA was spiked in with 300 ng genomic DNA in two-step real-time PCR with (+RT) and without (-RT) reverse transcriptase. Both mRNA and gDNA were detected with the human TUBA assay.

Amplification was detected from other kits (B, QuantiTect Reverse Transcription Kit (QIAGEN); C, Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase (Thermo Fisher Scientific); D, PrimeScript RT Reagent Kit with gDNA Eraser (Takara), indicating a significant amount of residual gDNA.

RFU, relative fluorescence units

iScript™ gDNA Clear cDNA Synthesis Kit: Sensitivity and Reproducibility

Outstanding reproducibility over 7 orders of magnitude even after DNase treatment.

iScript gDNA Clear cDNA Synthesis Kit’s Sensitivity and Reproducibility

Tenfold serial diluted Jurkat RNA, ranging from 1 µg to 1 pg, was used to generate cDNA subsequently used for qPCR using the human B2M mRNA specific assay.

A, amplification profile; B, standard curve

Consistent Real-Time PCR Performance Observed Even after gDNA Removal

No changes in PCR efficiency, sensitivity, and reproducibility were observed.

Sample Efficiency R2 Slope
Treated (red dot) 100.3% 0.999 -3.314
Untreated (blue square) 99.0% 0.997 -3.345

Extracted Jurkat RNA was reverse transcribed using the iScript™ gDNA Clear cDNA Synthesis Kit with and without DNase treatment.

Two-step real-time PCR, using the human B2M mRNA specific assay, was then performed on samples with and without gDNA removal to monitor its impact on real-time PCR data.

Understanding Reverse Transcription
Which iScript™ Kit is right for me?
 

Most
Convenient

Integrated
DNA
Removal

Maximum
Input Use

Flexible Priming,
High-Fidelity

Value
Solution

Product iScript Reverse Transcription Supermix for RT-qPCR iScript gDNA
Clear cDNA
Synthesis Kit
iScript Advanced cDNA Synthesis Kit for RT-qPCR iScript Select cDNA
Synthesis Kit
iScript cDNA
Synthesis Kit
Applications
  • Gene expression
  • Gene expression
  • Gene expression
  • Cloning
  • Sequencing and NGS
  • Gene expression
  • Gene expression
Total Input RNA 1 µg–1 pg 1 µg–1 pg 7.5 µg–100 fg 1 µg–1 pg 1 µg–100 fg
Format 1 tube 3 tubes 2 tubes 5 tubes 2 tubes
Kit Contents
  • 5x iScript RT Supermix
  • No-RT control supermix
  • 5x iScript RT Supermix
  • No-RT control supermix
  • DNase
  • DNase buffer
  • iScript Reverse Transcriptase
  • 5x iScript Advanced Reaction Mix
  • iScript Reverse Transcriptase
  • 5x iScript Reaction Mix
  • 3 priming options
  • iScript Reverse Transcriptase
  • 5x iScript Reaction Mix
Time to Produce cDNA 26 min 36 min 21 min 15–65 min 26 min
RNase H+ Activity High High High High High
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The iScript gDNA Clear cDNA Synthesis Kit integrates an effective DNase digestion step with fast and sensitive first-strand cDNA synthesis for accurate qPCR data.

This kit combines the iScript Reverse Transcription Supermix and a specially formulated DNase to effectively eliminate genomic DNA (gDNA) from purified RNA in 10 minutes. qPCR-ready cDNA free of contaminant gDNA is generated in 36 minutes.

Key Features and Benefits

  • Effective genomic DNA removal — without compromising cDNA synthesis efficiency
  • Proprietary DNase buffer — maintaining RNA integrity during gDNA removal
  • Convenient all-in-one, single-tube supermix — for reproducible and efficient cDNA synthesis
  • Ready-to-use no-RT control supermix provided — for monitoring gDNA contamination
  • Superior sensitivity and reproducibility — works with a broad linear dynamic range of input total RNA (1 µg–1 pg)
  • Unbiased qPCR data — consistent efficiency, sensitivity, and reproducibility in qPCR results even after gDNA removal

gDNA-free cDNA ready in 36 minutes
This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.

Workflow for DNA Removal and cDNA Synthesis

Importance of Addressing gDNA Contamination in RT-qPCR Gene Expression

gDNA contamination in RNA samples can be amplified in RT-qPCR, biasing the results for the transcript of interest. This problem is especially acute for low expressing genes — even a few copies of gDNA contamination can shift Cq values dramatically.

Benefit of Using Reverse Transcriptase with RNase H+ in RT-qPCR Gene Expression

RNase H works to degrade RNA that is hybridized to DNA. During cDNA synthesis, the RNase H domain of the Moloney Murine Leukemia Virus (MMLV) reverse transcriptase degrades the template RNA as the cDNA is synthesized.

This activity ensures the one-to-one conversion of RNA into cDNA molecules, resulting in more accurate gene expression data. Reverse transcriptase in all iScript reverse transcription reagents exhibits RNase H activity.

Choose the Right iScript Kit for Your Needs

Feature Integrated gDNA Removal Most Convenient Just Add RNA Maximum Input Use of up to 7.5 µg RNA Flexible Priming Options Value Solution
Product gDNA Clear cDNA Synthesis Kit Reverse Transcription Supermix Advanced cDNA Synthesis Kit Select cDNA Synthesis Kit cDNA Synthesis Kit
Total Input RNA 1 µg–1 pg 1 µg–1 pg 7.5 µg–100 fg 1 µg–1 pg 1 µg–100 fg
cDNA ready in 36 min 26 min 21 min 40–90 min 26 min

Related Products

All products listed below are compatible across real-time PCR instruments.

Generates reliable results, due to patented Sso7d fusion protein technology, in dye-based qPCR under various challenging conditions.

Performs efficiently in challenging singleplex and duplex qPCR reactions across a broad range of reaction conditions.

Produces consistent and reliable dye-based qPCR results.

Performs reliably and reproducibly with singleplex and duplex reactions.

More Information

Our Understanding Reverse Transcription Tutorial brings you product information, performance data, interactive tutorials, 3D animated videos, and more.

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iScript™ gDNA Clear cDNA Synthesis Kit, 25 x 20 µl reactions

1725034
25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 200 µl no-RT control supermix; 12.5 µl DNase; 150 µl DNase buffer

1725034 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725034_1443118822.xml 1725034 09/24/15 10:30 AM en_US true internet/product_SKU 2019-05-04 16:19:15 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725034_1443118822.xml /default/main/bio-rad/WORKAREA/default jdl0szbf 2017-11-14 07:07:36 true jdl0szbf templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725034_1443118822.xml LSR N /default/main/bio-rad en 09/25/25 11:20 AM 1555974594630 2019-05-04 16:19:15 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725034_1443118822.xml 1725034-iscript-gdna-clear-cdna-synthesis-kit-25-x-20-ul-reactions 1 1725034 iScript gDNA Clear cDNA Synthesis Kit, 25 x 20 ?l reactions iScript&trade; gDNA Clear cDNA Synthesis Kit, 25 x 20 µl reactions 25 x 20 &micro;l reactions, 100 &micro;l 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 200 &micro;l no-RT control supermix; 12.5 &micro;l DNase; 150 &micro;l DNase buffer 25 x 20 &micro;l reactions, 100 &micro;l 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 200 &micro;l no-RT control supermix; 12.5 &micro;l DNase; 150 &micro;l DNase buffer /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/iscript-gdna-clear-cdna-synthesis-kit-25-20-725034-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/iscript-gdna-clear-cdna-synthesis-kit-25-20-725034-view.jpg preview ,VN Karen Moss iScript&trade; gDNA Clear cDNA Synthesis Kit 25 x 20 &micro;l reactions, 100 &micro;l 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 200 &micro;l No-RT control supermix; 12.5 &micro;l DNase; 150 &micro;l DNase buffer 09/24/15 10:30 AM 09/25/25 11:20 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Effective gDNA removal in purified RNA samples</li> <li>Proprietary DNase buffer for maintaining RNA integrity during gDNA removal</li> <li>Convenient all-in-one, single-tube RT supermix</li> <li>Ready-to-use no-RT control supermix for monitoring gDNA contamination</li> <li>gDNA-free cDNA ready in 36 min</li> </ul> <h2 class="descHeader">gDNA-Free cDNA Ready in 36 Minutes</h2> <p>This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/workflow-for-dna-removal-and-cdna-synthesis.jpg" alt="Workflow for DNA Removal and cDNA Synthesis" width="599" height="146" /></p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Kit Size</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">25 x 20 &micro;l reactions</td> <td valign="top">1725034</td> </tr> <tr> <td valign="top">100 x 20 &micro;l reactions</td> <td valign="top"><a href="/en-us/sku/1725035-iscript-gdna-clear-cdna-synthesis-kit-100-x-20-ul-reactions">1725035</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">500 x 20 &micro;l reactions</td> <td valign="top"><a href="/en-us/sku/1725035bun-iscript-gdna-clear-cdna-synthesis-kit-500-x-20-ul-reactions?ID=1725035BUN">1725035BUN</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Supporting Documentation</h2> <p>Download the <a href="/webroot/web/pdf/lsr/literature/10043078.pdf" target="_blank" rel="noopener noreferrer">iScript gDNA Clear cDNA Synthesis Kit Product Insert</a> for detailed information about step-by-step protocols for cDNA synthesis and troubleshooting tips.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global iscript-gdna-clear-cdna-synthesis-kit-1725034-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/iscript-gdna-clear-cdna-synthesis-kit-1725034-desc.html Product expiration <br>(after shipping date) 2026 12 months 1 0 Storage 2198 Store DNase buffer at 4&deg;C upon receipt. Store all other kit components at &ndash;20&deg;C. 1 0 1725270 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5270_1361992326.xml 1725280 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5280_1362100008.xml 1708840 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8840_1284066528.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725034&CartQuote=QT&quantity= 1445 false true false $222.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725034&AddToDefaultWishList=true&quantity= true false true 41116100 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725034&quantity= 1725034
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iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions

1725035
100 x 20 µl reactions, 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 400 µl no-RT control supermix; 50 µl DNase; 150 µl DNase buffer

1725035 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725035_1443120615.xml 1725035 09/24/15 10:37 AM en_US true internet/product_SKU 2019-05-04 16:19:07 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725035_1443120615.xml /default/main/bio-rad/WORKAREA/default jdl0szba 2017-11-14 07:07:36 true jdl0szba templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725035_1443120615.xml LSR N /default/main/bio-rad en 09/24/25 11:49 AM 1555974594714 2019-05-04 16:19:07 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725035_1443120615.xml 1725035-iscript-gdna-clear-cdna-synthesis-kit-100-x-20-ul-reactions 1 1725035 iScript gDNA Clear cDNA Synthesis Kit, 100 x 20 ?l reactions iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions 100 x 20 &micro;l reactions, 400 &micro;l 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 400 &micro;l no-RT control supermix; 50 &micro;l DNase; 150 &micro;l DNase buffer 100 x 20 &micro;l reactions, 400 &micro;l 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 400 &micro;l no-RT control supermix; 50 &micro;l DNase; 150 &micro;l DNase buffer /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/iscript-gdna-clear-cdna-synthesis-kit-100-20-725035-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/iscript-gdna-clear-cdna-synthesis-kit-100-20-725035-view.jpg iScript&trade; gDNA Clear cDNA Synthesis Kit preview ,VN Karen Moss iScript&trade; gDNA Clear cDNA Synthesis Kit 100 x 20 &micro;l reactions, 400 &micro;l 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 400 &micro;l No-RT control supermix; 50 &micro;l DNase; 150 &micro;l DNase buffer 09/24/15 10:37 AM 09/24/25 11:49 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Effective gDNA removal in purified RNA samples</li> <li>Proprietary DNase buffer for maintaining RNA integrity during gDNA removal</li> <li>Convenient all-in-one, single-tube RT supermix</li> <li>Ready-to-use no-RT control supermix for monitoring gDNA contamination</li> <li>gDNA-free cDNA ready in 36 min</li> </ul> <h2 class="descHeader">gDNA-Free cDNA Ready in 36 Minutes</h2> <p>This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/workflow-for-dna-removal-and-cdna-synthesis.jpg" alt="Workflow for DNA Removal and cDNA Synthesis" width="599" height="146" /></p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Kit Size</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">25 x 20 &micro;l reactions</td> <td valign="top"><a href="/en-us/sku/1725034-iscript-gdna-clear-cdna-synthesis-kit-25-x-20-ul-reactions">1725034</a></td> </tr> <tr> <td valign="top">100 x 20 &micro;l reactions</td> <td valign="top">1725035</td> </tr> <tr class="pd_colorbackground"> <td valign="top">500 x 20 &micro;l reactions</td> <td valign="top"><a href="/en-us/sku/1725035bun-iscript-gdna-clear-cdna-synthesis-kit-500-x-20-ul-reactions?ID=1725035BUN">1725035BUN</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Supporting Documentation</h2> <p>Download the <a href="/webroot/web/pdf/lsr/literature/Bulletin_6742.pdf" target="_blank" rel="noopener noreferrer">iScript gDNA Clear cDNA Synthesis Kit Product Insert</a> for detailed information about step-by-step protocols for cDNA synthesis and troubleshooting tips.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global iscript-gdna-clear-cdna-synthesis-kit-1725035-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/iscript-gdna-clear-cdna-synthesis-kit-1725035-desc.html Product expiration <br>(after shipping date) 2026 12 months 1 0 Storage 2198 Store DNase buffer at 4&deg;C upon receipt. Store all other kit components at &ndash;20&deg;C. 1 0 1725270 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5270_1361992326.xml 1725280 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5280_1362100008.xml 1708840 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8840_1284066528.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725035&CartQuote=QT&quantity= 1445 false true false $552.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725035&AddToDefaultWishList=true&quantity= true false true 41116100 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725035&quantity= 1725035
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iScript™ gDNA Clear cDNA Synthesis Kit, 500 x 20 µl reactions

1725035BUN
500 x 20 µl reactions, 5 x 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 5 x 400 µl no-RT control supermix; 5 x 50 µl DNase; 5 x 150 µl DNase buffer

1725035BUN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725035BUN_1526667873.xml 1725035BUN 05/18/18 06:22 PM en_US true internet/product_SKU 2019-05-04 16:19:11 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725035BUN_1526667873.xml /default/main/bio-rad/WORKAREA/default jh81v6e1 2018-05-18 18:24:33 true jh81v6e1 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725035BUN_1526667873.xml LSR N /default/main/bio-rad en 05/18/28 08:23 AM 1555974594672 2019-05-04 16:19:11 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1725035BUN_1526667873.xml 1725035bun-iscript-gdna-clear-cdna-synthesis-kit-500-x-20-ul-reactions 1 1725035BUN Script gDNA Clear cDNA Kit 500 rxn iScript™ gDNA Clear cDNA Synthesis Kit, 500 x 20 µl reactions 500 x 20 µl reactions, 5 x 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 5 x 400 µl no-RT control supermix; 5 x 50 µl DNase; 5 x 150 µl DNase buffer /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/1725035bun_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/1725035bun_view.jpg iScript™ gDNA Clear cDNA Synthesis Kit, 500 x 20 µl reactions preview ,VN Tommy A iScript™ gDNA Clear cDNA Synthesis Kit, 500 x 20 µl reactions iScript, gDNA, RT, Reverse transcription, gDNA contamination, genomic DNA 05/18/18 06:22 PM 05/18/28 08:23 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Effective gDNA removal in purified RNA samples</li> <li>Proprietary DNase buffer for maintaining RNA integrity during gDNA removal</li> <li>Convenient all-in-one, single-tube RT supermix</li> <li>Ready-to-use no-RT control supermix for monitoring gDNA contamination</li> <li>gDNA-free cDNA ready in 36 min</li> </ul> <h2 class="descHeader">gDNA-Free cDNA Ready in 36 Minutes</h2> <p>This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/workflow-for-dna-removal-and-cdna-synthesis.jpg" alt="Workflow for DNA Removal and cDNA Synthesis" width="599" height="146" /></p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Kit Size</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">25 x 20 &micro;l reactions</td> <td valign="top"><a href="/en-us/sku/1725034-iscript-gdna-clear-cdna-synthesis-kit-25-x-20-ul-reactions">1725034</a></td> </tr> <tr> <td valign="top">100 x 20 &micro;l reactions</td> <td valign="top"><a href="/en-us/sku/1725035-iscript-gdna-clear-cdna-synthesis-kit-100-x-20-ul-reactions?ID=1725035">1725035</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">500 x 20 &micro;l reactions</td> <td valign="top">1725035BUN</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Supporting Documentation</h2> <p>Download the <a href="/webroot/web/pdf/lsr/literature/Bulletin_6742.pdf" target="_blank" rel="noopener noreferrer">iScript gDNA Clear cDNA Synthesis Kit Product Insert</a> for detailed information about step-by-step protocols for cDNA synthesis and troubleshooting tips.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> 1725035bun.html /1725035bun.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725035BUN&CartQuote=QT&quantity= 1445 false true false $2,517.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725035BUN&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725035BUN&quantity= 1725035BUN
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Number Description Options
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6746 iScript Reverse Transcription Kits Flier Click to download [ Add to Cart (Free) ]
6742 iScript™ gDNA Clear cDNA Synthesis Kit Product Information Sheet, Ver B Click to download
6763 Gene Expression Real-Time PCR Workflow Flier Click to download [ Add to Cart (Free) ]
6816 Protocol for Multiplex RT-qPCR Analysis Using PrimePCR™ Assays, Ver A Click to download
10043078 iScript™ gDNA Clear cDNA Synthesis Kit Product Insert, Ver B Click to download

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