iScript™ cDNA Synthesis Kit

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iScript cDNA Synthesis Kit

Overview

Broad Dynamic Range

The iScript™ cDNA synthesis kit performs across a broad range of concentrations. Input RNA (amounts shown in inset) was reverse transcribed and the resulting cDNA was amplified using iQ™ SYBR® Green supermix. The iScript cDNA synthesis kit performs over six orders of magnitude with high sensitivity. Standard curve r = 0.998, efficiency = 96.5%, RFU, relative fluorescence units.

Excellent Sensitivity

Sensitivity of the iScript™ cDNA synthesis kit in RT-PCR using low amounts of input RNA. cDNA was synthesized at 42°C and amplified with iTaq™ DNA polymerase using β-actin primers. M, EZ Load™ 100 bp molecular ruler; C, no-template negative control.

RNaseA Inhibition
 

 

 

RNase levels in different RNA samples. RNaseA, water, RT buffer (used to dilute RNaseA), spleen RNA (S RNA-1 and S RNA-2), and pancreas RNA (P RNA-1 and P RNA-2) from two different preparations were assayed for RNaseA activity using a commercially available kit. RNaseA (several fold higher than normally found in contaminated RNA preparations) was used as control to demonstrate varying levels of RNaseA activity in sample preparations.

 

RNase inhibition by the iScript™ cDNA synthesis kit. RNaseA, water, RT buffer (used to dilute RNaseA), and RNase + iScript cDNA kit were assayed for RNaseA activity using a commercially available kit. RNaseA (several fold higher than normally found in contaminated RNA preparations) was used as control to assess the effect of inhibition by the iScript cDNA kit.

 

Functional RT-qPCR assay demonstrating RNaseA inhibition by the iScript cDNA synthesis kit. 18S rRNA was amplified using iQ™ SYBR® Green supermix. Reverse transcription (RT) performed with iScript kit (); RT performed with spiked RNaseA (); RT performed with RNaseA but no RNase inhibitor (■). Two RNA inputs (1,000 and 0.1 pg) were reverse transcribed for all three groups. The cDNA synthesis reaction with RNase but no RNase inhibitor showed significant threshold cycle (CT) delay (3–5), whereas the iScript kit demonstrated powerful inhibition of RNaseA. RFU, relative fluorescence units.

Which iScript™ Kit is right for me?
 

Most
Convenient

Integrated
DNA
Removal

Maximum
Input Use

Flexible Priming,
High-Fidelity

Value
Solution

Product iScript Reverse Transcription Supermix for RT-qPCR iScript gDNA
Clear cDNA
Synthesis Kit
iScript Advanced cDNA Synthesis Kit for RT-qPCR iScript Select cDNA
Synthesis Kit
iScript cDNA
Synthesis Kit
Applications
  • Gene expression
  • Gene expression
  • Gene expression
  • Cloning
  • Sequencing and NGS
  • Gene expression
  • Gene expression
Total Input RNA 1 µg–1 pg 1 µg–1 pg 7.5 µg–100 fg 1 µg–1 pg 1 µg–100 fg
Format 1 tube 3 tubes 2 tubes 5 tubes 2 tubes
Kit Contents
  • 5x iScript RT Supermix
  • No-RT control supermix
  • 5x iScript RT Supermix
  • No-RT control supermix
  • DNase
  • DNase buffer
  • iScript Reverse Transcriptase
  • 5x iScript Advanced Reaction Mix
  • iScript Reverse Transcriptase
  • 5x iScript Reaction Mix
  • 3 priming options
  • iScript Reverse Transcriptase
  • 5x iScript Reaction Mix
Time to Produce cDNA 26 min 36 min 21 min 15–65 min 26 min
RNase H+ Activity High High High High High
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The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time.

Key Features and Benefits

  • Have ease of use and consistency — 2-tube (iScript reaction mix and iScript reverse transcriptase) format for faster setup
  • Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Utilize dilute RNA samples — reaction mix and iScript enzyme blend format allows up to 15 µl of RNA sample in a 20 µl cDNA synthesis reaction
  • Prevent 5' and 3' bias of your target genes and design primers in any region — optimum blend of oligo(dT) and random primers in the 5x supermix
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription
  • Complete cDNA synthesis and qPCR on the same day — fast 26 minute cDNA synthesis protocol
Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
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iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns

1708890
25 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

0 1708890 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml 1708890 09/21/09 12:05 PM en_US true internet/product_SKU 2018-03-06 03:19:37 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml /default/main/bio-rad/WORKAREA/default jcun051o 2017-11-14 07:07:38 true jcun051o templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml LSR N /default/main/bio-rad en 09/21/22 12:05 PM 1519715010486 2018-03-06 03:19:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml 1708890-iscript-cdna-synthesis-kit-25-x-20-ul-rxns 1 1708890 iScript cDNA Synthesis Kit iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns 25 x 20 &#181;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water 25 x 20 &#181;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 25 x 20 &#181;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8890_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8890_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8890_feature.jpg RepEx 170-8890 25 x 20 &#181;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 170-8890, 1708890, iscript, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection 09/21/09 12:05 PM 09/21/22 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Up to 15 &micro;l of RNA in a 20 &micro;l cDNA synthesis reaction</li> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Fast 26 min cDNA synthesis protocol</li> <li>Linear range of 1 &micro;g&ndash;1 pg total input RNA using RNase H+ MMLV reverse transcriptase</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;25</td> <td style="padding-left: 70px;" valign="top">&nbsp; 100 (1 x 100 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1708890</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 100</td> <td style="padding-left: 70px;" valign="top">&nbsp; 400 (1 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708891-iscript-cdna-synthesis-kit-100-x-20-ul-rxns"><span style="font-size: 11px;">1708891</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2,000 (5 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708891bun-iscript-cdna-synthesis-kit-500-x-20-ul-rxns"><span style="font-size: 11px;">1708891BUN</span></a></td> </tr> </tbody> </table> <br /><br /> <p>See the <a href="/en-us/product/iscript-cdna-synthesis-kit">iScript cDNA Synthesis Kit</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript cDNA Synthesis Kit <a href="/webroot/web/pdf/lsr/literature/4106228.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 170-8890_0921120538440_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//170-8890_0921120538440_en.html 1708842 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8842_1301949358.xml HSP9601 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/HSP-9601_0921120534821.xml MSC1001 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/MSC-1001_1297906204.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708890&CartQuote=QT&quantity= false true false $155.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708890&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708890&quantity= 1708890
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iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns

1708891
100 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

1 1708891 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8891_0921120538190.xml 1708891 09/21/09 12:05 PM en_US true internet/product_SKU 2018-03-06 03:19:37 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8891_0921120538190.xml /default/main/bio-rad/WORKAREA/default jcun050q 2017-11-14 07:07:38 true jcun050q templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8891_0921120538190.xml LSR N /default/main/bio-rad en 09/21/19 12:05 PM 1519715010536 2018-03-06 03:19:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8891_0921120538190.xml 1708891-iscript-cdna-synthesis-kit-100-x-20-ul-rxns 1 1708891 iScript cDNA Synthesis Kit iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns 100 x 20 &#181;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water 100 x 20 &#181;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 100 x 20 &#181;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8891_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8891_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8891_feature.jpg RepEx 170-8891 100 x 20 &#181;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 170-8891, 1708891, iscript, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Up to 15 &micro;l of RNA in a 20 &micro;l cDNA synthesis reaction</li> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Fast 26 min cDNA synthesis protocol</li> <li>Linear range of 1 &micro;g&ndash;1 pg total input RNA using RNase H+ MMLV reverse transcriptase</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;25</td> <td style="padding-left: 70px;" valign="top">&nbsp; 100 (1 x 100 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708890-iscript-cdna-synthesis-kit-25-x-20-ul-rxns"><span style="font-size: 11px;">1708890</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 100</td> <td style="padding-left: 70px;" valign="top">&nbsp; 400 (1 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1708891</span></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2,000 (5 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708891bun-iscript-cdna-synthesis-kit-500-x-20-ul-rxns"><span style="font-size: 11px;">1708891BUN</span></a></td> </tr> </tbody> </table> <br /><br /> <p>See the <a href="/en-us/product/iscript-cdna-synthesis-kit">iScript cDNA Synthesis Kit</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript cDNA Synthesis Kit <a href="/webroot/web/pdf/lsr/literature/4106228.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 170-8891_0921120538190_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//170-8891_0921120538190_en.html 1708842 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8842_1301949358.xml HSP9601 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/HSP-9601_0921120534821.xml MSC1001 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/MSC-1001_1297906204.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891&CartQuote=QT&quantity= false true false $453.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891&quantity= 1708891
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iScript™ cDNA Synthesis Kit, 500 x 20 µl rxns

1708891BUN
500 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

1708891BUN /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8891BUN_1318355597.xml 1708891BUN 10/11/11 10:44 AM en_US true internet/product_SKU 2018-03-06 03:19:36 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8891BUN_1318355597.xml /default/main/bio-rad/WORKAREA/default jcun05hm 2017-11-14 07:07:36 true jcun05hm templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8891BUN_1318355597.xml LSR N /default/main/bio-rad en 10/11/21 10:44 AM 1519714504060 2018-03-06 03:19:36 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8891BUN_1318355597.xml 1708891bun-iscript-cdna-synthesis-kit-500-x-20-ul-rxns 1 1708891BUN iScript cDNA Synthesis Kit (500 reactions) iScript™ cDNA Synthesis Kit, 500 x 20 µl rxns 500 x 20 &micro;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water 500 x 20 &micro;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8891BUN_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8891BUN_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8891BUN_feature.jpg Karen Moss iScript&trade; cDNA Synthesis Kit (500 reactions) 500 x 20 &micro;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 170-8891BUN, 1708891BUN, iscript, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection, rt-qpcr 10/11/11 10:44 AM 10/11/21 10:44 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Up to 15 &micro;l of RNA in a 20 &micro;l cDNA synthesis reaction</li> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Fast 26 min cDNA synthesis protocol</li> <li>Linear range of 1 &micro;g&ndash;1 pg total input RNA using RNase H+ MMLV reverse transcriptase</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;25</td> <td style="padding-left: 70px;" valign="top">&nbsp; 100 (1 x 100 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708890-iscript-cdna-synthesis-kit-25-x-20-ul-rxns"><span style="font-size: 11px;">1708890</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 100</td> <td style="padding-left: 70px;" valign="top">&nbsp; 400 (1 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708891-iscript-cdna-synthesis-kit-100-x-20-ul-rxns"><span style="font-size: 11px;">1708891</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2,000 (5 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1708891BUN</span></td> </tr> </tbody> </table> <br /><br /> <p>See the <a href="/en-us/product/iscript-cdna-synthesis-kit">iScript cDNA Synthesis Kit</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript cDNA Synthesis Kit <a href="/webroot/web/pdf/lsr/literature/4106228.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 170-8891BUN_desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/170-8891BUN_desc.html &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891BUN&CartQuote=QT&quantity= false true false $2,132.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891BUN&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891BUN&quantity= 1708891BUN
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4106228 iScript™ cDNA Synthesis Kit Product Insert, Ver D Click to download [ Add to Cart (Free) ]
6746 iScript Reverse Transcription Kits Flier Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download [ Add to Cart (Free) ]

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