iQ™ SYBR® Green Supermix

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Overview

Quantitation and Precision

iQ™ SYBR® Green supermix generates precise, quantitative results. A 5-fold dilution series (50 ng–80 pg) of human genomic DNA was amplified using the supermix and primers and a probe specific to the IL-1β gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ® real-time system. r = 0.999, slope = –3.380, efficiency = 97.6%, RFU, relative fluorescence units.

Broad Dynamic Range

iQ™ SYBR® Green supermix and the iScript cDNA synthesis kit show high specificity over a broad dynamic range of cDNA. Serial dilutions (1 µg–1 pg) of HeLa total RNA were reverse transcribed, and the resulting cDNA was amplified using primers specific to the β-actin gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ® real-time system. The consistent spacing of the curves reflects accurate reverse transcription and amplification. r = 0.997, slope = -3.615, efficiency = 89.1%, RFU, relative fluorescence units.

Specificity With Plasmid DNA

iQ™ SYBR® Green supermix shows high specificity with plasmid templates. Tenfold serial dilutions (108 to 102 copies) of plasmid DNA were amplified using primers specific to the β-actin gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ® real-time system. r = 1.000, slope = -3.492, efficiency = 93.4%, RFU, relative fluorescence units.

iQ™ SYBR® Green supermix is formulated to achieve optimal results for a wide array of real-time PCR applications, with the convenience of SYBR® Green I detection chemistry. This preblended 2x solution contains an optimized concentration of SYBR® Green I dye for sensitive and accurate detection, a hot-start iTaq™ DNA polymerase, a proprietary buffer and stabilizers specifically optimized for qPCR using SYBR® Green I, and qPCR-qualified dNTPs. This supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Use of iQ™ SYBR® Green supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude.

Key Features and Benefits

  • Increased throughput — requires minimal preparation since only template and primers are needed
  • High sensitivity and accuracy — the antibody-mediated, hot-start iTaq DNA polymerase minimizes the amplification of nonspecific PCR products and allows sensitive and accurate detection of as few as 10 copies of template
  • High specificity over a broad dynamic range — the combination of the iQ™ SYBR® Green supermix with the iScript™ cDNA synthesis kit delivers effective results over a broad dynamic range of cDNA

Applications and Uses

  • PCR and real-time PCR analysis
  • Nucleic acid amplification and expression profiling
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications
Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C Guaranteed for 6 months at 4°C (protected from light)
iQ™ SYBR® Green Supermix

170-8880
2.5 ml (2 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 100 x 50 µl reactions

List Price:   $183.00
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iQ™ SYBR® Green Supermix

170-8882
12.5 ml (10 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 500 x 50 µl reactions

List Price:   $819.00
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iQ™ SYBR® Green Supermix

170-8884
25 ml (20 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 µl reactions

List Price:   $1,585.00
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iQ™ SYBR® Green Supermix

170-8884BUN
50 ml (40 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

List Price:   $3,108.00
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iQ™ SYBR® Green Supermix

170-8885
50 ml (1 x 50 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

List Price:   $3,108.00
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iQ™ SYBR® Green Supermix

170-8886
25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 μl reactions

List Price:   $1,470.00
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iQ™ SYBR® Green Supermix

170-8887
50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions

List Price:   $2,730.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
4106212 Instruction Manual, iQ SYBR Green Supermix, 100 x 50 µl, Rev D Click to download [ Add to Cart (Free) ]
2764 iQ Supermixes for qPCR, Rev E Click to download
10016680 iQ SYBR Green Supermix User Guide, Rev A Click to download
5990 Gene Expression Analysis Solutions Brochure, Rev C Click to download [ Add to Cart (Free) ]