iQ™ Supermix

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Overview

Extreme Precision

An accurate one-cycle spacing in CT is precisely maintained in a series of 2-fold dilutions. Human genomic DNA was amplified with iQ™ supermix, using primers and a probe specific to the IL-1β gene. Eight replicates at each template concentration were amplified along with no-template controls on the MyiQ™ real-time system. r = 0.999, slope = –3.378, efficiency = 97.7%, RFU, relative fluorescence units.

Accurate RT-qPCR

iQ™ supermix and the iScript™ cDNA synthesis kit accomplish accurate, quantitative two-step RT-PCR. Serial dilutions (1 µg–1 pg) of HeLa total RNA were reverse transcribed, and the resulting cDNA was amplified using primers and a probe specific to the α-tubulin gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ® real-time system. The consistent 3.3-cycle spacing demonstrates the accuracy of amplification with this supermix as well as the fidelity of the iScript kit in reverse transcribing the 10-fold dilutions of the input RNA. r = 0.999, slope = –3.304, efficiency = 100.8%, RFU, relative fluorescence units.

Broad Dynamic Range

iQ™ supermix provides sensitive real-time detection over 8 orders of magnitude. Tenfold dilutions of a plasmid containing 109 to 10 copies of the α-tubulin gene were amplified using iQ supermix and a FAM-labeled hybridization probe for detection. Eight replicates at each concentration were amplified along with no-template controls on the MyiQ™ real-time system. r = 0.999, slope = –3.365, efficiency = 98.2%, RFU, relative fluorescence units.

iQ™ supermix is specially formulated for real-time PCR and has been tested for reliable amplification over a wide dynamic range of human genomic and plasmid DNA concentrations. It contains hot-start iTaq™ DNA polymerase, dNTPs qualified for quantitative PCR, and buffer at concentrations optimal for real-time PCR assays. iQ supermix facilitates reaction optimization and is qualified for use with all Bio-Rad real-time detection systems.

Key Features

  • Increased throughput — only template, primers, and probes are needed
  • Convenient reaction assembly at room temperature
  • Minimized contamination with easy-to-use formulation
Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C Guaranteed for 6 months at 4°C
iQ™ Supermix

170-8860
100 x 50 µl reactions, 2x real-time PCR mix, contains dNTPs, 6 mM MgCl2, 50 U/ml hot-start iTaq™ DNA polymerase

List Price:   $179.00
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iQ™ Supermix

170-8862
500 x 50 µl reactions, 2x real-time PCR mix, contains dNTPs, 6 mM MgCl2, 50 U/ml hot-start iTaq™ DNA polymerase

List Price:   $799.00
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iQ™ Supermix

170-8864
1,000 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2

List Price:   $1,547.00
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iQ™ Supermix
iQ™ Supermix

170-8865
2,000 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2

List Price:   $3,034.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
4106204 Instruction Manual, iQ Supermix PCR Reagents, Rev D Click to download
2764 iQ Supermixes for qPCR, Rev E Click to download
5894 Highly Efficient Reverse siRNA Transfection of Human Epithelial Lung Carcinoma A549 Cells Using siLentFect Lipid Reagent, Rev A Click to download