iQ™ Multiplex Powermix

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Overview

Accurate Quantitation of Five Targets

iQ™ multiplex powermix produces highly reliable qPCR results for up to five targets in a single tube, with no difference in detection of a low-expressing gene in multiplex or singleplex. One-tenth of a 1 µg cDNA synthesis reaction of human thymus total RNA was used in each 20 µl reaction. Top: (green), FAM-labeled β-actin probe, (orange), Cy5-labeled α-tubulin probe, (blue), HEX-labeled GAPDH probe, (pink), TAMRA-labeled cyclophilin probe, (red), Texas Red-labeled IL-2 probe. Bottom: (dark green squares), Texas Red-labeled IL-2 probe singleplex reactions, (red squares), Texas Red-labeled IL-2 probe multiplex reactions, RFU, relative fluorescence units.

iQ™ multiplex powermix simplifies real-time detection of multiple targets in a single tube. Although design software, such as Beacon Designer software, has made it easier to design effective primers and probes, finding a set of reaction conditions that amplifies all targets with equal efficiency in both singleplex and multiplex reactions can still be a challenge. iQ multiplex powermix makes multiplex real-time PCR easier by removing the need to optimize buffer, enzyme, or primer concentrations. This mix ensures:

  • Reliable real-time multiplex detection of up to 5 targets
  • Linearity over 6 orders of magnitude of input cDNA and 4 orders of magnitude of input genomic DNA
  • Detection of up to 4 targets when one differs in expression up to 106-fold relative to the others

With this reliable mix, you can increase throughput and control costs by running multiple assays in a single reaction, maximizing the amount of data collected from limited amounts of sample. iQ multiplex powermix is formulated for analysis using cDNA, genomic DNA, and plasmids, and can be used for a wide variety of applications, including:

  • Gene expression analysis
  • SNP genotyping/SNP analysis
  • GMO detection
  • Viral load detection

dUTP Discontinuation Notice

Bio-Rad supermixes containing dUTP have been discontinued and replaced with mixes containing dTTP. The reasons for eliminating dUTP from these supermixes are:

  • The high specificity of PCR and quantitative PCR (qPCR) and the use of disposable consumables and filter tips enable the elimination of dUTP and UNG from typical qPCR processes
  • All researchers in a particular facility must use dUTP and UNG for them to be effective; if any researcher is not using them, they will not be effective controls
  • dTTP mixes offer better efficiency, especially for GC-rich templates
  • dUTP and UNG add significant cost to performing qPCR, since UNG must be purchased separately
  • dTTP-containing mixes maintain superb quality and high performance in qPCR
Product expiration (after mfg date) 12 months
Customer guarantee (minimum usage time) 6 months
Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C Guaranteed for 6 months at 4°C
iQ™ Multiplex Powermix

172-5848
50 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl2, iTaq™ DNA polymerase

List Price:   $148.00
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iQ™ Multiplex Powermix

172-5849
200 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl2, iTaq™ DNA polymerase

List Price:   $543.00
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Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
10010601 Instruction Manual, iQ Multiplex Powermix, Rev A Click to download
5348 iQ Multiplex Powermix Flier, Rev C Click to download