iQ-Check® STEC Kits

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Overview

Key Benefits

Faster Results

  • Test results within hours after 10 hr enrichment
  • Rapid virulence gene screening of up to 94 samples

Easy To Use

  • Easy one step DNA extraction
  • Automated amplification and detection
  • Automatic result interpretation

Minimal Risk

  • Kits developed to comply with USDA FSIS testing requirements
  • Positive and negative PCR controls, plus an internal amplification control
  • Specific primers and probes

A complete Solution

  • Standardized Bio-Rad reagents:
  • Enrichment media
  • iQ-Check kits
  • Chromogenic agars
  • Validated Bio-Rad equipment and software for automated data analysis
Protocol

Validation

iQ-Check™ STEC is certified by:

  • AOAC-RI (No. 121203)

Bio-Rad has received a Letter of No Objection from the USDA for the indicated use of the iQ-Check® STEC Kit.

The iQ-Check STEC Kit consists of two test kits, iQ-Check STEC VirX and iQ-Check STEC SerO, based on the fast, sensitive, and proven technology of real-time PCR.

The iQ-Check STEC VirX Kit allows the separate detection of stx1-stx2 and eae virulence genes, in a single well, according the ISO TS 13136 or the USDA FSIS MLG5B.02 standard methods. In case of positive results, starting from the same DNA extract, the iQ-Check STEC SerO Kit enables the detection of the Big 6 STEC (O26, O111, O45, O145, O121, and O103) plus E. coli O157:H7.

The method is intended for detecting STEC virulence genes and 7 major STEC serogroups in raw beef meats, raw milk products, produce and environmental samples.

Due to the sensitivity and specificity of PCR, detection of the virulence genes can be obtained in only few hours following microbial enrichment. The enrichment is as short as 10 hr for raw meat samples, and up to 15 hr for other samples.

Highly Specific and Reliable

The iQ-Check STEC kits use an optimized system of primers and patented probes to ensure high specificity. Both kits are multiplex kits and allow the detection of several targets in a single well, including an internal positive amplification control, which validates any negative result.

Maximum Flexibility

Developed as a flexible solution, iQ-Check STEC VirX Kits can be used for up to 94 samples and iQ-Check STEC SerO Kit for up to 30 samples. The two kits can be run in parallel, on Bio-Rad’s high-throughput and multiplex real-time PCR systems

A Complete Solution

Bio-Rad offers a complete solution including enrichment media, real-time PCR kits, real-time PCR detection systems, in addition to software for automated analysis.

iQ-Check® STEC VirX Kit

357-8139
96 reactions

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iQ-Check® STEC SerO Kit

357-8140
32 reactions

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mTSB Broth dehydrated (base) #356-4426
mTSB Broth dehydrated (base)

356-4426
500 g

List Price:   $99.00
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STEC Enrichment Broth
STEC Enrichment Broth

356-4001
500 g

List Price:   $89.00
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STEC Enrichment Broth

356-4002
5 kg

List Price:   $892.00
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Number Description Options
FSD_16439 iQ-Check® Real-Time PCR Methods for Food and Environmental Testing Brochure, Rev E Click to download
FSD_16565 iQ-Check® STEC Kit Brochure, Rev B Click to download
FSD_808475 Instruction Manual, iQ-Check® STEC SerO Kit, Rev C Click to download
FSD_808474 Instruction Manual, iQ-Check® STEC VirX Kit, Rev B Click to download
FSD_200 iQ-Check® STEC SerO Kit Quick Guide: Using the CFX Manager™ IDE Software Click to download
FSD_194 iQ-Check® STEC VirX Kit Quick Guide: Standard II Extraction Deepwell Protocol – Raw Beef Samples, Rev B Click to download
PF_193 iQ-Check® STEC VirX Kit Quick Guide: Easy II Extraction Deepwell Protocol – Raw Beef Samples, Rev B Click to download
PF_243 iQ-Check® Stec VirX Kit Quick Guide: Easy I Extraction Deepwell Protocol – Raw Milk Products, Produce, and Environmental Samples, Rev A Click to download
FSD_16096 iQ-Check® Prep Solution Brochure, Rev A Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download