iQ-Check® Salmonella II PCR Detection Kits 

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Overview


Key Benefits

Faster Results

  • Test results within hours after 16 hr enrichment in buffered peptone water
  • Ultra-rapid screening of raw meat samples: results in 12 hr
  • Rapid screening of up to 94 samples

Easy To Use

  • Simple DNA extraction
  • Automated amplification and detection
  • Ready-to-use reagents, including for DNA extraction

Minimal Risk

  • Positive and negative PCR controls, plus an internal inhibition control
  • Specific primers and probes

A complete Solution

  • Standardized Bio-Rad reagents:
    • Enrichment media
    • iQ-Check kits
    • Chromogenic agars
  • Validated Bio-Rad equipment and software for automated data analysis



Protocol

Validation

    


iQ-Check™ Salmonella is certified by :

  • AFNOR certification according to the ISO 16140 requirements (No BRD 07/06 – 07/04)
  • AOAC-RI (No 010803)
  • NordVal Validation #38
  • Health Canada validation (MFLP-38)


The iQ-Check Salmonella II PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for detecting Salmonella spp. in animal feed and environmental samples, as well as in food samples such as poultry, eggs, and raw meats.

Due to the sensitivity and specificity of PCR, results can be obtained in little as 12 hr for raw meat samples and in 20 hr for all other samples following a single enrichment in a nonselective medium.

Highly Specific and Reliable

The iQ-Check Salmonella II Kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Salmonella II Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.

Validated Protocols

The iQ-Check Salmonella II protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls
Assay type PCR
Organism Salmonella spp.
x

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iQ-Check Salmonella II Kit #357-8123
iQ-Check® Salmonella II Kit

3578123
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 reactions

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iQ-Check® Free DNA Removal Solution

3594970
10 ml, iQ-Check Free DNA Removal Reagent, 1 vial (175 mg lyophilized powder), iQ-Check 10x Activation Buffer, 1 tube (9 ml)

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$490.00
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Buffered Peptone Water Broth, ready to use

3554179
Pkg of 6, 225 ml bottles

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$27.00
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Buffered Peptone Water Broth, dehydrated

3564684
Pkg of 1, 500 g

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$58.00
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(Discontinued) Buffered Peptone Water Broth, ready to use

3555790
Pkg of 2, 5 L bags

This product is no longer available. Please contact technical support for more information on replacement product.
RAPID'Salmonella Capsules, 10x concentrated

3564709
Pkg of 100 capsules, each enough for 2.5 L enrichment broth for 250 g sample

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$555.00
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RAPID'Salmonella Capsules

3564710
Pkg of 100 capsules, each enough for 250 ml enrichment broth for 25 g sample

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$64.00
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RAPID' Salmonella Supplement Box
(Discontinued) RAPID'Salmonella Agar, Supplement Box

3564712
Pkg of 1, enough for 100 analyses

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
FSD_13-0265 iQ-Check® Salmonella II Kit Brochure Click to download
808463 iQ-Check® Salmonella II Kit User Guide, Rev G Click to download
FSD_16310 iQ-Check® Salmonella II Kit Quick Guide – Easy Extraction Tube Protocol, Rev B Click to download
PF_069 iQ-Check® Salmonella II Kit Quick Guide: Standard Extraction II - Fast Protocol for Raw Meats, Rev B Click to download
PF_175 iQ-Check® Salmonella II Kit Quick Guide: Easy II Extraction – Meat Products, Rev A Click to download
FSD_PF185 iQ-Check® Salmonella II Kit Quick Guide: Standard II Extraction - Primary Production Samples, Rev A Click to download
PF_091 iQ-Check® Salmonella II Kit Quick Guide: Easy I Extraction Deepwell Protocol, Rev C Click to download
FSD_PF091TMX iQ-Check® Salmonella II Kit Quick Guide: Easy I Extraction Using the Thermomixer, Rev C Click to download
FSD_PF092 iQ-Check® Salmonella II Kit Quick Guide: Standard Extraction II - Raw Meat Samples, Rev C Click to download
TN_Salmonella_Spice Internal Comparative Evaluation Study of Alternative Enrichment Protocols for Detection of Salmonella in Untreated Spices, Ver A Click to download
10000058391 iQ-Check® Free DNA Removal Solution Product Insert, Ver A Click to download
FSD_16096 iQ-Check® Prep Solution Brochure, Rev A Click to download
FSD_16439 iQ-Check® Real-Time PCR Solution Brochure, Rev H Click to download
6834 iQ-Check® Free DNA Removal Solution Brochure, Ver A Click to download
FSD_16539 iQ-Check® Metodi PCR in Tempo Reale per Analisi Alimentazione e Ambientali Opuscolo, Rev B (Italiano) Click to download
FSD_16540 iQ-Check® Echtzeit-PCR Methoden zur Lebensmittelanalytik und Umwelttests Broschüre, Rev B (Deutsch) Click to download
FSD_16541 iQ-Check® Métodos PCR en Tiempo Real para Análisis de Alimentos y Pruebas Ambientales Folleto, Rev B (Español) Click to download
FSD_16542 iQ-Check® Méthodes PCR en Temps Réel pour Tests d'Alimentation et Environnementaux Brochure, Rev B (Français) Click to download
FSD_16543 iQ-Check® Métodos PCR em Tempo Real para Testes Alimentares e Ambientais Brochura, Rev B (Português) Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download