iQ-Check Salmonella II Kit 

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Overview


Key Benefits

Faster Results

  • Test results within hours after 16 hr enrichment in buffered peptone water
  • Ultra-rapid screening of raw meat samples: results in 12 hr
  • Rapid screening of up to 94 samples

Easy To Use

  • Simple DNA extraction
  • Automated amplification and detection
  • Ready-to-use reagents, including for DNA extraction

Minimal Risk

  • Positive and negative PCR controls, plus an internal inhibition control
  • Specific primers and probes

A complete Solution

  • Standardized Bio-Rad reagents:
    • Enrichment media
    • iQ-Check kits
    • Chromogenic agars
  • Validated Bio-Rad equipment and software for automated data analysis



Protocol

Validation

    


iQ-Check™ Salmonella is certified by :

  • AFNOR certification according to the ISO 16140 requirements (No BRD 07/06 – 07/04)
  • AOAC-RI (No 010803)
  • NordVal Validation #38

The iQ-Check® Salmonella II kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for detecting Salmonella spp. in animal feed and environmental samples, as well as in food samples such as poultry, eggs, and raw meats.

Due to the sensitivity and specificity of PCR, results can be obtained in little as 12 hr for raw meat samples and in 20 hr for all other samples following a single enrichment in a nonselective medium.

Highly Specific and Reliable

The iQ-Check Salmonella II kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Salmonella II kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check tests can also be run in parallel.

Validated Protocols

The iQ-Check Salmonella II protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time.

 A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

 Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls
Assay type PCR
Organism Salmonella spp.
iQ-Check Salmonella II Kit #357-8123
iQ-Check™ Salmonella II Kit

357-8123
96 reactions

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Buffered Peptone Water/Broth

355-5790
5 L x 2 bags

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Buffered Peptone Water/Broth

355-4179
225 ml x 6 bottles

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Buffered Peptone Water/Broth

356-4684
500 g

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iQ-Check™ S. Enteritidis Kit

357-8142
96 reactions

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RAPID´Salmonella Capsules, 10 x concentrated

356-4709
100 capsules

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RAPID´Salmonella Capsules

356-4710
100 capsules

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RAPID' Salmonella Supplement Box
RAPID'Salmonella Supplement Box

356-4712
1 Box q.s.p. 100 analysis

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Number Description Options
FSD_16325 iQ_Check Salmonella Click to download
FSD_16439 iQ-Check Real-Time PCR Methods for Food and Environmental Testing Brochure Click to download
FSD_16310 Quick Guide iQ-Check Salmonella II - Easy Extraction (revB) Click to download
FSD_PF092 Quick Guide iQ-CheckSalmonella II Standard Extraction II - Deepwell Protocol Raw Meat Samples (revB) Click to download
FSD_PF185 PF185_IQCSalmo Click to download
808463 iQ-Check Salmonella II Kit User Guide Click to download