iQ-Check Listeria spp. Kit

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Overview


Key Benefits

Faster Results

  • Test results within hours after 22 hr enrichment
  • Rapid screening of up to 94 samples

Easy To Use

  • Simple DNA extraction
  • Automated amplification and detection
  • Ready-to-use reagents, including for DNA extraction

Minimal Risk

  • Positive and negative PCR controls, plus an internal inhibition control
  • Specific primers and probes

A complete Solution

  • Standardized Bio-Rad reagents:
  • Enrichment media
  • iQ-Check kits
  • Chromogenic agars
  • Validated Bio-Rad equipment and software for automated data analysis



Protocol

            

iQ-Check™ Listeria spp is certified by:

  • AFNOR certification according to the ISO 16140 requirements (No BRD 07/13-05/07)
  • AOAC-RI (No 090701)
  • Health Canada validation (MFLP-39)

The iQ-Check® Listeria spp. kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for the detection of Listeria spp. in environmental samples and in food samples such as dairy products and fish.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 24 hr following a single enrichment in a selective medium.

Highly Specific and Reliable

The iQ-Check Listeria spp. kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Listeria spp. kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check tests can also be run in parallel.

Validated Protocols

The iQ-Check Listeria spp. kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time.

 A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls
Assay type PCR
Organism Listeria spp.
iQ-Check Listeria spp. kit #357-8113
iQ-Check™ Listeria spp. Kit

357-8113
96 reactions

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LSB (Listeria Special Broth)

355-5703
Broth, 225 ml x 6 bottles

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LSB (Listeria Special Broth)

356-4703
500 g

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Number Description Options
FSD_16327 iQ Check Listeria spp. Click to download
FSD_16439 iQ-Check Real-Time PCR Methods for Food and Environmental Testing Brochure Click to download
FSD_16275 Quick Guide iQ-Check Listeria spp. - Easy Extraction Click to download
FSD_PF098 Quick Guide iQ-Check Listeria spp - Easy Extraction Deepwell Protocol Click to download
808465 iQ-Check Listeria spp. Kit User Guide, Rev E Click to download
TS_LSB TS_Listeria_Special_Broth Click to download