iQ-Check® Enterobacteriaceae PCR Detection Kit

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iQ-Check Enterobacteriaceae Kit

Overview

Key Benefits

Faster Results

  • Test results within hours after 16 hr enrichment
  • Rapid screening of up to 94 samples

Easy To Use

  • Simple DNA extraction
  • Automated amplification and detection
  • Ready-to-use reagents, including for DNA extraction

Minimal Risk

  • Positive and negative PCR controls, plus an internal inhibition control
  • Specific primers and probes

A Complete Solution

  • Standardized Bio-Rad reagents:
  • Enrichment media
  • iQ-Check Kits
  • Chromogenic agars
  • Validated Bio-Rad equipment and software for automated data analysis


Enterobacteriaceae (EB), often used as indicators for hygiene process criteria, is a large family of gram-negative bacteria that encompasses some of the most well-known food pathogens like Salmonella, Cronobacter, or pathogenic E. coli. For thermal processed food or ingredients, this provides an indication of the efficiency of inactivation treatment, but also as a prevention of recontamination. Testing of total EB is based on the standard ISO 21528-1 method, which is labor intensive and requires at least 2 days for the screening of negative samples.

The method is intended for the detection of Enterobacteriaceae in infant formula and related raw ingredients as well as environmental samples. The method is also a complement to our iQ-Check Cronobacter Solution.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 3 hr following a single enrichment in non-selective buffered peptone water (3564684 and 3554179).

Highly Specific and Reliable

iQ-Check Enterobacteriaceae Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation). The iQ-Check Free DNA Removal Solution completes and optimizes the method by removing free DNA from food and environmental samples.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Enterobacteriaceae Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check Assays can be run in parallel on the same plate using the iQ-Check Prep Automation System.

A Complete Solution

Bio-Rad offers a complete solution of standardized reagents, such as enrichment media, PCR kits, confirmation agars, PCR detection systems, and software for automated analysis specific for each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls
Organism Enterobacteriaceae
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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

iQ-Check Enterobacteriaceae Kit
iQ-Check® Enterobacteriaceae PCR Detection Kit

12003068
Pkg of 1, kit includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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iQ-Check® Free DNA Removal Solution

3594970
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

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$490.00
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Buffered Peptone Water Broth, ready to use

3554179
Pkg of 6, 225 ml bottles

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$27.00
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Buffered Peptone Water Broth, dehydrated

3564684
Pkg of 1, 500 g

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$58.00
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Buffered Peptone Water Broth, ready to use

3555795
Pkg of 4, 3L bags

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$112.00
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Buffered Peptone Water Broth, ready to use

3555790
Pkg of 2, 5 L bags

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$112.00
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RAPID'Enterobacteriaceae Agar, ready to use

3554012
Pkg of 6, 200 ml bottles

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$68.00
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RAPID'<em>Enterobacteriaceae</em> Agar, dehydrated
RAPID'Enterobacteriaceae Agar, dehydrated

3564004
Pkg of 1, 500 g of dehydrated agar broth, enough for 13.5 L of medium

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$120.00
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VRBG (Violet Red Bile Glucose)/Agar

3564584
500 g

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$98.00
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VRBG (Violet Red Bile Glucose)/Agar

3554239
100 ml x 6 bottles

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$26.00
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Number Description Options
10000081305 iQ-Check® Enterobacteriaceae Kit User Guide Click to download
FSD_16439 iQ-Check® Real-Time PCR Solution Brochure, Rev H Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
10000058391 iQ-Check® Free DNA Removal Solution Product Insert, Ver A Click to download
6834 iQ-Check® Free DNA Removal Solution Brochure, Ver A Click to download