iQ-Check E.coli O157:H7 Kit

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Overview

Key Benefits

Faster Results

  • Test results within hours after 8 hr enrichment in BPW
  • Rapid screening of up to 94 samples

Easy To Use

  • No IMS step
  • Simple DNA extraction
  • Ready-to-use reagents, including for DNA extraction

Minimal Risk

  • No cross-reactions with E.coli O55
  • Screening with no isolated colonies
  • Positive and negative PCR controls, plus an internal inhibition control

A Complete Solution

  • Standardized Bio-Rad reagents:
    • Enrichment media
    • iQ-Check kits
    • Chromogenic agars
  • Validated Bio-Rad equipment and software for automated data analysis



Protocol
Validation

iQ-Check® E.coli O157:H7 is certified by:

  • AFNOR certification according to the ISO 16140 requirements (No BRD 07/15-06/08)
  • AOAC-RI (No. 020801)

The iQ-Check® E. coli O157:H7 kit is based on the fast, sensitive, and proven technology of real-time PCR. It is intended for detecting E. coli O157:H7 in food samples such as raw beef and fresh produce.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 12 hr after enrichment in nonselective medium.

Highly Specific and Reliable

The iQ-Check test uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions with close strains such as E. coli O55. The test is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check E. coli O157:H7 kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check tests can also be run in parallel.

Validated Protocols

The iQ-Check E. coli O157:H7 kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls
Assay type PCR
Organism E. coli O157:H7
iQ-Check E.coli O157:H7 #357-8114
iQ-Check™ E.coli O157:H7 Kit

357-8114
96 reactions

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Buffered Peptone Water/Broth

355-4179
225 ml x 6 bottles

List Price:   $25.00
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Buffered Peptone Water/Broth

356-4684
500 g

List Price:   $58.00
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STEC Enrichment Broth
STEC Enrichment Broth

356-4001
500 g

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STEC Enrichment Broth

356-4002
5 kg

List Price:   $892.00
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Number Description Options
FSD_16328 iQ check E.coli O157 Click to download
FSD_16439 iQ-Check Real-Time PCR Methods for Food and Environmental Testing Brochure Click to download
FSD_PF094 FSD_PF094 Click to download
FSD_16390 Quick Guide iQ-Check E.coli O157:H7 - Easy Extraction (all foods) - (RevB) Click to download
808466 iQ-Check E.coli O157:H7 Kit User Guide Click to download