Cronobacter, formerly known as Enterobacter sakazakii, is a ubiquitous pathogen that has been isolated from food, environmental and clinical samples. It is associated with rare but fatal infant infections linked to the consumption of reconstituted powdered infant formula.
The iQ-Check Cronobacter spp. PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for the detection of Cronobacter spp. in food, mainly infant formula and baby food, and environmental samples.
Due to the sensitivity and specificity of PCR, results can be obtained in as little as 16 hr following a single enrichment in nonselective buffered peptone water (3564684) (3554179).
The iQ-Check Cronobacter spp. Kit uses an optimized system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.
Developed as an open and flexible system, the iQ-Check Cronobacter spp. Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.
Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.
- Lysis reagent for DNA extraction
- Fluorescent probes
- PCR amplification mix
- Positive and negative PCR controls