Individual Autoantibody EIA Profiling

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Bio-Rad offers a full range of individual autoantibody EIA assays. The assays feature common protocols, common reagents, color-coded breakaway wells and easy automation, allowing for simple, objective and sensitive autoimmune diagnostics. These assays can be used in conjunction with ANA and ENA assays to identify specific autoantibodies associated with autoimmune diseases. Bio-Rad also offers the Kallestad Anti-dsDNA EIA Kit, a quantitative assay with a 5-point calibrator.

Bio-Rad Autoimmune EIA Assay Characteristics

  • Sample dilution: 1:40 (only Anti-dsDNA and Anti-ssDNA are 1:100)
  • Sample volume: 10 µL
  • Incubation time: 90 minutes (30/30/30)
  • Incubation temperature: Room temperature (18–27°C)
  • Conjugate: HRP
  • Substrate: TMB

Kallestad Anti-dsDNA EIA Assay Characteristics

  • Sample dilution: 1:100
  • Sample volume: 10 µL
  • Incubation time: 120 minutes (60/30/30)
  • Incubation temperature: Room temperature (18–25°C)
  • Conjugate: Alkaline phosphatase
  • Substrate: Phenolphthaleinmonophosphate (PMP)

 

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Bio-Rad Anti-SS-A EIA Kit

96SA
Semi-quantitative enzyme immunoassay for detection of autoantibodies specific for SS-A antigen (96 tests)

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Bio-Rad Anti-SS-B EIA Kit

96SB
Semi-quantitative enzyme immunoassay for detection of autoantibodies specific for SS-B antigen (96 tests)

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Bio-Rad Anti-Sm EIA Kit

96SM
Semi-quantitative enzyme immunoassay for detection of autoantibodies specific for Sm antigen (96 tests)

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Bio-Rad Anti-Sm/RNP EIA Kit

96SR
Semi-quantitative enzyme immunoassay for detection of antibodies specific for Sm and RNP antigens (96 tests)

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Bio-Rad Anti-Jo-1 EIA Kit

96JO
Semi-quantitative enzyme immunoassay for detection of autoantibodies specific for Jo-1 antigen (96 tests)

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Bio-Rad Anti-Scl-70 EIA Kit

96SC
Semi-quantitative enzyme immunoassay for detection of autoantibodies specific for Scl-70 antigen (96 tests)

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Bio-Rad Anti-Centromere EIA Kit

96CE
Semi-quantitative enzyme immunoassay for detection of IgG autoantibodies against the centromere antigen (96 tests)

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Bio-Rad Anti-dsDNA EIA Kit (1-Point Calibrator)

96DS
Enzyme immunoassay for double-stranded DNA antibodies involved in systemic rheumatic diseases; standardized against WHO Ref. Prep. Wo/80 (96 tests)

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Bio-Rad Anti-ssDNA EIA Kit

96SS
Semi-quantitative enzyme immunoassay for detection of autoantibodies to single-stranded DNA (96 tests)

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Bio-Rad Kallestad Anti-dsDNA EIA Kit (5-Point Calibrator)

31008
Qualitative or quantitative enzyme immunoassay for detection of autoantibodies to double-stranded DNA (96 tests)

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