Immun-Star™ AP Chemiluminescence Kits

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Overview

Chemiluminescence Chemistry

Chemiluminescence detection. The secondary antibody, which binds the antibody specific for the protein of interest, is conjugated to an enzyme; when it acts on the substrate, light is emitted.

Immun-Star™ AP Signal

Long-lasting Immun-Star AP signal, shown by a serial dilution of goat anti-rabbit (GAR) antibody on a slot blot. X-ray film exposures of 10 sec were made at the time of initial substrate activation (lane 1), 6 hr (lane 2), 12 hr (lane 3), and 24 hr (lane 4).



Chemiluminescent western blot detection is a highly sensitive alternative to isotopic detection of proteins bound to blotting membranes. Instead of radioactively labeled antibodies, enzyme-conjugated secondary antibodies produce a light signal while acting on the substrate. An enhancer intensifies the signal output, enabling the detection of picogram quantities of antigen. Also, it increases the duration of the light signal up to 6–8 hr. The light signal can be captured on film or an imaging system. The light signal continues 24 hr after initial activation, which allows flexibility in obtaining data. Exposure times on film are typically 30 sec to 5 min, depending on sample amount and antibody specificity. The Immun-Star™ AP chemiluminescence kit is therefore a highly sensitive, fast-acting alternative to isotopic detection.

These kits combine Bio-Rad's blotting reagents and expertise with CDP-Star chemiluminescence technology. Additional features of Immun-Star AP kits include:

  • Working solution stability for 24 hr at room temperature
  • Compatibility with both PVDF and nitrocellulose membranes
  • Ability to reactivate a blot, even weeks later, with the addition of fresh chemiluminescent substrate
  • Detection of as little as 10 pg of protein, comparable to the sensitivity of radioactive methods
  • Possibility of stripping and reprobing

Two kits are available, based on goat anti-mouse or goat anti-rabbit conjugates.

 

Lower detection limit Picogram
Signal duration 24 hr
Primary detection method Film
Suggested antibody dilution Primary: 1/1,000–1/6,000
Secondary: 1/3,000
Recommended membrane Nitrocellulose or PVDF
Shelf life 12 months at 4°C
Immun-Star™ AP Substrate

170-5018
125 ml, alkaline phosphatase substrate for 2,500 cm2 of membrane (~50 mini blots)

List Price:   $240.00
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Immun-Star™ Goat Anti-Mouse (GAM)-AP Detection Kit

170-5010
Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate; for 2,500 cm2 of membrane (~50 mini blots)

List Price:   $297.00
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Immun-Star™ Goat Anti-Rabbit (GAR)-AP Detection Kit

170-5011
Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate; for 2,500 cm2 of membrane (~50 mini blots)

List Price:   $297.00
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Immun-Star™ AP Substrate Pack

170-5012
Pkg of 1, alkaline phosphatase substrates and enhancer for 2,500 cm2 of membrane (~50 mini blots)

List Price:   $271.00
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Goat Anti-Rabbit (GAR)-AP Intro Kit

170-5014
Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate, reagents; for 400 cm2 of membrane (~8 mini blots)

List Price:   $117.00
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Blotting Reagents Pack

170-5015
Pkg of 1, TBS, Tween 20, nonfat dry milk for 2,500 cm2 of membrane (~50 mini blots)

List Price:   $107.00
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Number Description Options
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
4006074 Instruction Manual, Immun-Star Chemiluminescent Protein Detection System, Rev D Click to download