Immun-Blot® PVDF Membrane

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Overview

Available Formats

Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

PVDF Membranes

Ideal for:

  • Protein blotting
    (Immun-Blot® PVDF membrane)
  • Protein sequencing
    (Sequi-Blot™ PVDF membrane)

 

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.

Immun-Blot® PVDF membrane is ideal for chemiluminescent and colorimetric western blots: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.

Features and Benefits

  • Mechanical strength of Immun-Blot PVDF membrane enables multiple reprobing of blots
  • Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
  • Available in three formats:
    • Convenient precut membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Transferring proteins from SDS-PAGE gels to membrane
  • Immunoblotting target proteins
  • Detection by western blotting chemiluminescent or colorimetric methods

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

 

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Immun-Blot PVDF Membrane #162-0177
Immun-Blot® PVDF Membrane, Roll, 26 cm x 3.3 m

1620177
Pkg of 1 roll, 0.2 µm, 26 cm x 3.3 m, bulk membrane for high binding (150–160 µg/cm2) immunoblotting

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$315.00
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Immun-Blot PVDF Membrane #162-0176
Immun-Blot® PVDF Membrane, Precut, 20 x 20 cm

1620176
Pkg of 10, 0.2 µm, 20 x 20 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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$240.00
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Immun-Blot PVDF Membrane #162-0175
Immun-Blot® PVDF Membrane, Precut, 10 x 15 cm

1620175
Pkg of 10, 0.2 µm, 10 x 15 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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$115.00
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Immun-Blot PVDF Membrane #162-0174
Immun-Blot® PVDF Membrane, Precut, 7 x 8.4 cm

1620174
Pkg of 10, 0.2 µm, 7 x 8.4 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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$84.00
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Immun-Blot PVDF/Filter Paper Sandwiches #162-0238
Immun-Blot® PVDF/Filter Paper Sandwiches

1620238
Pkg of 20, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels

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$320.00
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Immun-Blot PVDF/Filter Paper Sandwiches #162-0239
Immun-Blot® PVDF/Filter Paper Sandwiches

1620239
Pkg of 50, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels

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$644.00
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Immun-Blot PVDF/Filter Paper Sandwiches #162-0218
Immun-Blot PVDF/Filter Paper Sandwiches

1620218
Pkg of 20, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels

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$205.00
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Immun-Blot PVDF/Filter Paper Sandwiches #162-0219
Immun-Blot PVDF/Filter Paper Sandwiches

1620219
Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels

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$475.00
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Immun-Blot PVDF Membrane #162-0255
Immun-Blot® PVDF Membrane, Precut, 25 x 28 cm

1620255
Pkg of 10, 0.2 µm, 25 x 28 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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$238.00
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Number Description Options
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
2212 Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C Click to download
1939 Blotting Membrane Brochure, Rev G Click to download [ Add to Cart (Free) ]
4006127 Instruction Manual, Immun-Blot PVDF, Rev A Click to download