IEP/IEF Agaroses

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IEP/IEF Agaroses




Bio-Rad offers a selection of agaroses suitable for IEP, IEF, and other specialty applications.

Standard low –mr agarose — With its high strength, clarity, and low –mr value, this agarose is recommended for all standard immunoelectrophoresis applications. The powder may be used alone or blended with other grades of agarose for special properties.

High –mr agarose — The –mr value of ≥0.25 makes this gel suitable for counterimmunoelectrophoresis and to blend with standard low –mr agarose to increase its –mr for special applications.

Zero –mr agarose — This agarose is specifically for isoelectric focusing. It has no detectable electroendosmosis, and is recommended for isoelectric focusing of very high molecular weight proteins or complexes that are subject to varying degrees of molecular sieving in polyacrylamide gels. Agarose IEF and post-run processing can be completed more quickly than polyacrylamide gel IEF.

Electroendosmosis
–mr (1.0% Gel)
 
Gel Strength
(g/cm2 of a 1.0% Gel)
Standard low –mr ≤0.15 Standard low –mr ≥1,000
High –mr ≥0.25 High –mr ≥450
Zero –mr ≤0.02 Zero –mr ≥700
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Standard Low -mr Agarose for Immunoelectrophoresis (IEP)

1620100
100 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications

0 1620100 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0100_0921120414720.xml 1620100 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:44 1620100-standard-low-mr-agarose-for-immunoelectrophoresis-iep 1620100 Standard Low -mr Agarose Standard Low -m<sub>r</sub> Agarose for Immunoelectrophoresis (IEP) 100 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications 100 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications 100 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0100_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0100_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0100_feature.jpg Standard Low -m<sub>r</sub> Agarose for Immunoelectrophoresis (IEP) preview RepEx 162-0100 100 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications 1620100, standard, low, -mr, agarose, iep, ief, isolelectric, focusing, gel, electrophoresis, casting, immunoelectrophoresis, less, than, 0.15 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Standard Low -m<sub>r</sub> Agarose for immunoelectrophoresis applications. Low electroendosmosis (EEO) agarose reduces internal convection providing higher resolution separations.</p> <ul> <li>Low EEO (-m<sub>r</sub>) of a 1.0% gel &le;0.15</li> <li>Gel strength of a 1.0% gel &ge;1,000 g/cm<sup>2</sup></li> <li>Can be blended with other grades of agarose for different properties</li> <li>High strength and clarity </li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: center;" width="87" valign="top"><strong>Weight</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">100 g</td> <td style="text-align: center;" valign="top">1620100</td> </tr> <tr> <td style="text-align: center;" valign="top">500 g</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1620102-standard-low-mr-agarose-for-immunoelectrophoresis-iep?ID=1620102">1620102</a></td> </tr> </tbody> </table> <p class="caption" style="margin-top:-20px">&nbsp;</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Coomassie Brilliant Blue R-250 (<a href="/en-us/sku/1610400-coomassie-brilliant-blue-r-250?ID=1610400">1610400</a>)</li> <li>Coomassie Brilliant Blue G-250 (<a href="/en-us/sku/1610406-coomassie-brilliant-blue-g-250?ID=1610406">1610406</a>)</li> <li><a href="/en-us/product/readyprep-protein-extraction-kit-total-protein">ReadyPrep&trade; Protein Extraction Kit (Total Protein)</a></li> <li><a href="/en-us/product/proteominer-protein-enrichment-kits">ProteoMiner&trade; Protein Enrichment Kits </a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/buffers-reagents-acrylamide-for-protein-electrophoresis">Buffers, Reagents, and Acrylamide for Protein Electrophoresis</a><a href="/en-us/product/iep-ief-agaroses"></a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel Imaging Systems</a> </li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1620100.html /webroot/web/html/lsr/products/SKUs/autopopulated/1620100.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620100&CartQuote=QT&quantity= false true false $175.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620100&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620100&quantity= 1620100
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Standard Low -mr Agarose for Immunoelectrophoresis (IEP)

1620102
500 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications

1 1620102 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0102_0921120414741.xml 1620102 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:44 1620102-standard-low-mr-agarose-for-immunoelectrophoresis-iep 1620102 Standard Low -mr Agarose Standard Low -m<sub>r</sub> Agarose for Immunoelectrophoresis (IEP) 500 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications 500 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications 500 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0102_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0102_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0102_feature.jpg Standard Low -m<sub>r</sub> Agarose for Immunoelectrophoresis (IEP) preview RepEx 162-0102 500 g, -mr value of ≤0.15, recommended for all standard immunoelectrophoresis applications 1620102, standard, low, -mr, agarose, iep, ief, isolelectric, focusing, gel, electrophoresis, casting, immunoelectrophoresis, less, than, 0.15 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Standard Low -m<sub>r</sub> Agarose for immunoelectrophoresis applications. Low electroendosmosis (EEO) agarose reduces internal convection providing higher resolution separations.</p> <ul> <li>Low EEO (-m<sub>r</sub>) of a 1.0% gel &le;0.15</li> <li>Gel strength of a 1.0% gel &ge;1,000 g/cm<sup>2</sup></li> <li>Can be blended with other grades of agarose for different properties</li> <li>High strength and clarity </li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: center;" width="87" valign="top"><strong>Weight</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">100 g</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1620100-standard-low-mr-agarose-for-immunoelectrophoresis-iep?ID=1620100">1620100</a></td> </tr> <tr> <td style="text-align: center;" valign="top">500 g</td> <td style="text-align: center;" valign="top">1620102</td> </tr> </tbody> </table> <p class="caption" style="margin-top:-20px">&nbsp;</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Coomassie Brilliant Blue R-250 (<a href="/en-us/sku/1610400-coomassie-brilliant-blue-r-250?ID=1610400">1610400</a>)</li> <li>Coomassie Brilliant Blue G-250 (<a href="/en-us/sku/1610406-coomassie-brilliant-blue-g-250?ID=1610406">1610406</a>)</li> <li><a href="/en-us/product/readyprep-protein-extraction-kit-total-protein">ReadyPrep&trade; Protein Extraction Kit (Total Protein)</a></li> <li><a href="/en-us/product/proteominer-protein-enrichment-kits">ProteoMiner&trade; Protein Enrichment Kits </a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/buffers-reagents-acrylamide-for-protein-electrophoresis">Buffers, Reagents, and Acrylamide for Protein Electrophoresis</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel Imaging Systems</a> </li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1620102.html /webroot/web/html/lsr/products/SKUs/autopopulated/1620102.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620102&CartQuote=QT&quantity= false true false $659.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620102&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620102&quantity= 1620102
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(Discontinued)High -mr Agarose

1620001
50 g, -mr value of ≥0.25 for counter-immunoelectrophoresis

This product is no longer available. Please contact technical support for more information on replacement product.
Zero -mr Agarose for Isoelectric Focusing (IEF)

1620022
10 g, agarose for isoelectric focusing, -mr value of ≤0.02

3 1620022 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0022_0921120414699.xml 1620022 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:44 1620022-zero-mr-agarose-for-isoelectric-focusing-ief 1620022 Zero -mr Agarose Zero -m<sub>r</sub> Agarose for Isoelectric Focusing (IEF) 10 g, agarose for isoelectric focusing, -mr value of ≤0.02 10 g, agarose for isoelectric focusing, -mr value of ≤0.02 10 g, agarose for isoelectric focusing, -mr value of ≤0.02 /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0022_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0022_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0022_feature.jpg Zero -m<sub>r</sub> Agarose for Isoelectric Focusing (IEF) preview RepEx 162-0022 10 g, agarose for isoelectric focusing, -mr value of ≤0.02 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Choose Zero -m<sub>r</sub> Agarose for isoelectric focusing (IEF) of very high molecular weight proteins or complexes that will be retarded in polyacrylamide gels. Zero electroendosmosis (EEO) agarose reduces internal convection providing higher resolution separations.</p> <ul> <li>No detectable EEO (-m<sub>r</sub>) eliminates gradient drift in the neutral pH region</li> <li>Both focusing and postrun processing are faster than IEF using polyacrylamide</li> <li>Gel strength of a 1.0% gel &ge;700 g/cm<sup>2</sup></li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li>ReadyPrep&trade; 2-D Starter Kit (<a href="/en-us/sku/1632105-readyprep-2-d-starter-kit">1632105</a>)</li> <li><a href="/en-us/product/2-d-electrophoresis/readyprep-2-d-cleanup-kit">ReadyPrep&trade; 2-D Cleanup Kit</a></li> <li>CHAPS (<a href="/en-us/sku/1610460-chaps">1610460</a>)</li> <li><em>E. coli</em> Protein Sample, IEF control (<a href="/en-us/sku/1632110-e-coli-protein-sample">1632110</a>)</li> <li>Coomassie Brilliant Blue R-250 (<a href="/en-us/sku/1610400-coomassie-brilliant-blue-r-250?ID=1610400">1610400</a>)</li> <li>Coomassie Brilliant Blue G-250 (<a href="/en-us/sku/1610406-coomassie-brilliant-blue-g-250?ID=1610406">1610406</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/product/ampholytes">Ampholytes</a></li> <li><a href="/en-us/product/reducing-alkylating-agents">Reducing and Alkylating Agents</a></li> <li><a href="/en-us/product/silver-stains-kits">Silver Stains and Kits</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel Imaging Systems</a></li> </ul> <h2 class="descHeader">Related Applications</h2> <ul> <li><a href="/en-us/applications-technologies/2-d-electrophoresis-analysis">2-D Electrophoresis and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1620022.html /webroot/web/html/lsr/products/SKUs/autopopulated/1620022.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620022&CartQuote=QT&quantity= false true false $170.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620022&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620022&quantity= 1620022
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