Herpes simplex (HSV)

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Herpes Simplex Virus (HSV)

Herpes Simplex Virus 1/2 DFA

Pathfinder® HSV Identification in Direct Clinical Specimen Results by Investigator

  TP FP FN TN Inadequate Total No. (%) Total
Pathfinder Reagents + +    
Cell Culture Method + +    
Investigator A 34 0 6 18 11 (16%) 69
Investigator B 21 1 6 18 3 (6%) 49
In-house 26 0 8 7 6 (13%) 47
Total 81 1 20 43 20 165
Sensitivity = TP/(TP + FN) = 80% Specificity = TN/(TN + FP) = 98%


HSV Identification in Direct Clinical Specimen Results by Sex and Site of Lesion

      Predictive Value
Site of Lesion Male Female Sensitivity
TP/(TP + FN)
Specificity
TN/(TN + FP)
Positive
TP/(TP + FP)
Negative
TN/(TN + FN)
Genital 69   79%
38/48
95%
20/21
97%
38/39
67%
20/30
Genital   43 81%
25/31
100%
12/12
100%
25/25
67%
12/18
Anal 8 3 71%
5/7
100%
4/4
100%
5/5
67%
4/6
Other 10 11 86%
12/14
100%
7/7
100%
12/12
78%
7/9
Unknown 1          
Total 88 57  


Note: The calculations in the two tables are based on comparison to cell culture as the reference method.
TP = true positive, FP = false positive, FN = false negative, TN = true negative
.

Bio-Rad’s Pathfinder® HSV 1 and 2 DFA kit provides direct antigen detection and differentiation of herpes simplex virus (HSV) types 1 and 2 using fluorescein-conjugated monoclonal antibodies. Two results are available from one test in one hour. This test kit allows room temperature incubation and confirmation of cell culture isolates. The monoclonal antibody-based test can be used to directly stain clinical specimens or tissue culture cells. Flexible ordering options are available.

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Pathfinder™ HSV Types 1 and 2
Pathfinder™ HSV Types 1 and 2

25215
Direct antigen detection and differentiation of Herpes simplex virus (HSV) types 1 and 2 in direct clinical specimens and cell culture isolates (50 tests)

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HSV Collection Slide
HSV Collection Slide

30494
Collection slides for use with Herpes simplex virus (HSV) types 1 and 2 test kits (90 slides)

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Pathfinder™ HSV Control Slide
Pathfinder™ HSV Control Slide

30493
Control slides with Herpes simplex virus (HSV) to use as a positive control for HSV 1 and 2 test kits (4 wells)

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Pathfinder™ Mounting Medium
Pathfinder™ Mounting Medium

30693
Slide mounting medium for use with Pathfinder™ test kits (2.75 mL)

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